Thermo-acoustic imaging with the current injection (TAI-CI) is an emerging medical electrical parameter imaging technology for early detection of human diseases, it combines the advantages of high contrast of electrical impedance imaging and high resolution of ultrasound imaging. The principle of thermoacoustic imaging with the current injection was studied and the experimental system was established. The study results demonstrate that the joule heating is produced inside the objects by injecting current to phantom through a pair of electrodes, and an acoustic signal which involves the electrical parameter information of the objects is excited due to heat expansion. An experimental set for TAI-CI was established to detect and collect the acoustic signals from the semicircular ring gel phantom, the experiments were carried out aiming at low conductivity phantom (1S/m), the electrical parameter variation of the gel phantom was reflected through image reconstruction. The experiments verify the feasibility of the method for low conductivity medium imaging, and provides a basis for potential applications of TAI-CI in biological tissues.