Resolvin D1 attenuates the inflammatory process in mouse model of LPS‐induced keratitis

Petrillo, Francesco; Trotta, Maria Consiglia; Bucolo, Claudio; Petrillo, Arianna; Maisto, Rosa; Pieretti, Gorizio; Pietropaolo, Michela; Ferraraccio, Franca; Gagliano, Caterina; Galdiero, Marilena; D’Amico, Michele

doi:10.1111/jcmm.15633

Cited by 12 publications

(6 citation statements)

References 44 publications

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“…Intrastromal injection of 2 μg of Lipopolysaccharide (LPS, Sigma) from Pseudomonas aeruginosa, dissolved in 2 μL of phosphate-buffered saline (PBS, Sigma), was performed in both the eyes of male CD-1 mice under direct microscopic observation, according to previous studies [ 23 , 24 , 25 ]. Briefly, CD-1 mice were anaesthetized by pentobarbital (45 mg/kg in saline, i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…An investigator unaware of the treatments evaluated the development of keratitis by using a biomicroscope. As previously described [ 24 , 27 ], keratitis was scored as follows: 0 = normal, clear cornea, no inflammatory reaction; 1 = mild corneal haze with visible iris; 2 = moderate corneal flare with moderate corneal haze and superficial punctate keratitis; 3 = significant corneal opacity, infiltration of cells in the stroma; and 4 = damage and loss of corneal tissue. Positive keratitis was scored as >1.…”

Section: Methodsmentioning

confidence: 99%

“…Corneas were paraffin-embedded and cut in 5μm serial sections [ 28 ], then the paraffin was removed by a xylene substitute (Hemo-De; Thermo-Fisher Scientific, Darmstadt, Germany). The immunohistochemistry procedure was performed as previously described [ 24 ] by BenchMark Automated IHC/ISH slide staining system (BenchMarkVentana, Tucson, AZ, USA). Sections were incubated with specific anti-ki67 (Rabbit polyclonal anti-Ki67 antibody; concentration 5 μg/mL; ab155807 Abcam) and anti-VEGF (Mouse monoclonal anti-VEGF antibody; 1:100; sc-57496, Santa Cruz) antibodies.…”

Section: Methodsmentioning

confidence: 99%

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N-palmitoyl-D-glucosamine, A Natural Monosaccharide-Based Glycolipid, Inhibits TLR4 and Prevents LPS-Induced Inflammation and Neuropathic Pain in Mice

Iannotta

Belardo

Trotta

et al. 2021

IJMS

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Toll-like receptors (TLRs) are key receptors through which infectious and non-infectious challenges act with consequent activation of the inflammatory cascade that plays a critical function in various acute and chronic diseases, behaving as amplification and chronicization factors of the inflammatory response. Previous studies have shown that synthetic analogues of lipid A based on glucosamine with few chains of unsaturated and saturated fatty acids, bind MD-2 and inhibit TLR4 receptors. These synthetic compounds showed antagonistic activity against TLR4 activation in vitro by LPS, but little or no activity in vivo. This study aimed to show the potential use of N-palmitoyl-D-glucosamine (PGA), a bacterial molecule with structural similarity to the lipid A component of LPS, which could be useful for preventing LPS-induced tissue damage or even peripheral neuropathies. Molecular docking and molecular dynamics simulations showed that PGA stably binds MD-2 with a MD-2/(PGA)3 stoichiometry. Treatment with PGA resulted in the following effects: (i) it prevented the NF-kB activation in LPS stimulated RAW264.7 cells; (ii) it decreased LPS-induced keratitis and corneal pro-inflammatory cytokines, whilst increasing anti-inflammatory cytokines; (iii) it normalized LPS-induced miR-20a-5p and miR-106a-5p upregulation and increased miR-27a-3p levels in the inflamed corneas; (iv) it decreased allodynia in peripheral neuropathy induced by oxaliplatin or formalin, but not following spared nerve injury of the sciatic nerve (SNI); (v) it prevented the formalin- or oxaliplatin-induced myelino-axonal degeneration of sciatic nerve. SIGNIFICANCE STATEMENT We report that PGA acts as a TLR4 antagonist and this may be the basis of its potent anti-inflammatory activity. Being unique because of its potency and stability, as compared to other similar congeners, PGA can represent a tool for the optimization of new TLR4 modulating drugs directed against the cytokine storm and the chronization of inflammation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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N-palmitoyl-D-glucosamine, A Natural Monosaccharide-Based Glycolipid, Inhibits TLR4 and Prevents LPS-Induced Inflammation and Neuropathic Pain in Mice

Iannotta

Belardo

Trotta

et al. 2021

IJMS

Self Cite

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“…Consistent with these previous results, our data increased Ki67 expression in the control group; however, such increase was reversed by SA treatment. 35, 36 (Figure 5). LPS is well known as a proven NLRP3 inflammasome activator.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effect by Cloves Treatment in LPS-induced RAW264.7 Cells

Seo

Kim

Shin

2023

Pharmacognosy Magazine

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Background: Cloves mean the dried flower bud harvested from a tree of Syzygium aromaticum L. (SA). Objectives: The current study was to identify the anti-inflammatory effects of SA on lipopolysaccharide (LPS) (0.2 µg/mL)-treated RAW264.7 cells. Materials and Methods: WST-8 cell proliferation assay kit (WST-8) and lactate dehydrogenase (LDH) assays were completed to observe cell proliferation and cytotoxicity effects of SA. Other biochemical analyses analyzed releases of nitric oxide (NO) and the production of dichlorofluorescin diacetate (DCF-DA). Besides, inflammation-related factors and Sirt1 were carried out through immunohistochemistry (IHC) and immunofluorescence (IF). Results: LPS exposure not only caused abnormal elevations of both NO and reactive oxygen species (ROS) secretions, but also markedly increased inflammation-related proteins including inducible NO synthase (iNOS), cluster of differentiation (CD) 14, and toll-like receptor (TLR) 4, and nuclear factor-κB (NF-κB). The above-listed alterations were notably attenuated by SA pretreatment in a dose-dependent manner. We further figured out that, SA particularly activated AMPKα/ Sirt1 signaling pathway. Furthermore, SA dramatically inhibited interleukin (IL)-1β production, which was mediated by NLR family Pyrin domain containing (NLRP) 3 mediated inflammasome signaling pathway. Conclusion: SA inhibits LPS-induced inflammation in RAW264.7 cells via the TRL4/CD14/NF-κB pathway and it can lead to the reduction of IL-1β release by the inhibition of NLRP3 inflammasome. Such an anti-inflammatory effect was closely related to AMPKα/Sirt1 activation. Taken together, the findings of this study support both the inhibition of NLRP3 inflammasome and activation of Sirt1 can alleviate inflammation.

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“…Bathina and Das found that RvD1 can prevent streptozotocin-induced type 1 diabetes by reducing oxidative stress and suppressing inflammation [ 10 ]. In mouse model of lipopolysaccharide-induced keratitis, RvD1 was reported to ameliorate the inflammatory process [ 11 ]. In addition, PD1 was also found to enhance resolution of inflammation in lipopolysaccharide-induced acute lung injury [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Resolvin D1 and Protectin D1 as Potential Therapeutic Agents for Treating Kidney Stones

Wang

Wei

Huangfu

et al. 2022

Oxidative Medicine and Cellular Longevity

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Intrarenal calcium oxalate (CaOx) crystals induce renal tubular epithelial cell (TEC) inflammatory and oxidative injury. This study is aimed at exploring potential therapeutic lipid components in kidney stones because lipids are involved in the development of several diseases and indicate the risk of kidney stones. Serum specimens were collected from 35 kidney stone patients and 35 normal controls. The lipid components in serum were measured, and differences were analyzed. The documented biological importance was comprehensively reviewed to identify lipids that differed significantly between the two groups to find potential agents associated with kidney stones. CaOx nephrocalcinosis mouse model was established to examine the therapeutic effects of specific lipids on CaOx deposition and CaOx-induced oxidative renal injury. Several lipids with significantly different levels were present in the serum of patients with stones and normal controls. Resolvin D1 (RvD1) (4.93-fold change, P < 0.001 ) and protectin D1 (PD1) (5.06-fold change, P < 0.001 ) were significantly decreased in the serum of patients with kidney stones, and an integrative review suggested that these factors might be associated with inflammatory responses, which is a crucial mechanism associated with stone damage. The administration of RvD1 and PD1 significantly inhibited kidney CaOx deposition and suppressed CaOx-induced renal tubular cell inflammatory injury and necrosis in a CaOx nephrocalcinosis mouse model. Furthermore, RvD1 and PD1 facilitated the expression of the oxidative indicator superoxide dismutase 2 (SOD2), inhibited NADPH oxidase 2 (NOX2) expression, and diminished intracellular reactive oxygen species (ROS) levels. This study preliminarily elucidated the role of lipids in kidney stones. The inhibitory effects of RvD1 and PD1 on oxidative damage induced by CaOx deposition provide a promising perspective for kidney stone treatment strategies.

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Resolvin D1 attenuates the inflammatory process in mouse model of LPS‐induced keratitis

Cited by 12 publications

References 44 publications

N-palmitoyl-D-glucosamine, A Natural Monosaccharide-Based Glycolipid, Inhibits TLR4 and Prevents LPS-Induced Inflammation and Neuropathic Pain in Mice

N-palmitoyl-D-glucosamine, A Natural Monosaccharide-Based Glycolipid, Inhibits TLR4 and Prevents LPS-Induced Inflammation and Neuropathic Pain in Mice

Anti-inflammatory Effect by Cloves Treatment in LPS-induced RAW264.7 Cells

Identification of Resolvin D1 and Protectin D1 as Potential Therapeutic Agents for Treating Kidney Stones

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