Resolvin E1 Ameliorates Pulpitis by Suppressing Dental Pulp Fibroblast Activation in a Chemerin Receptor 23-dependent Manner

Xu, Huaxing; Chen, Jie; Ge, Jianping; Xia, Kun; Tao, Shuo; Su, Yiyi; Zhang, Qi

doi:10.1016/j.joen.2019.05.005

Cited by 31 publications

(39 citation statements)

References 34 publications

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“…The in uence of RvE1 on these in ammatory factors has been previously reported. Xu et al reported that RvE1 ameliorated pulpitis by inhibiting the expression of the proin ammatory cytokines IL-6 and TNF-α in a chemerin receptor23 (ChemR23)-dependent manner [28]. Rey et al reported that RvE1 decreased LPSinduced gene expression of proin ammatory cytokines (TNF-α and IL-6), suggesting that they exerted proresolutive activity in microglia [29].…”

Section: Discussionmentioning

confidence: 99%

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

Zhang

Niu

et al. 2020

Preprint

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Background: In addition to the direct effects of irradiation, the induced inflammatory response may play an important role in the damage to the inner ear caused by radiotherapy for the treatment of head and neck cancers. Resolvin E1 (RvE1) has anti-inflammatory activity, acting by reducing neutrophil infiltration and proinflammatory cytokine expression. Therefore, in this study we sought to confirm whether the inflammation induced by irradiation was involved in damage to the inner ear after radiotherapy and to investigate the protective effect and underlying mechanism of RvE1 using mouse models. Methods: A dose of RvE1 was delivered by intraperitoneal injection to mice before irradiation. Changes in the auditory brainstem response (ABR), relative balance ability, inner ear morphology and the expression levels of inflammatory factors in the inner ear were analyzed on days 7 and 14 after irradiation and compared among different experimental groups. Results: Changes of ABR and relative balance ability showed the inner functions of experimental mice presented severe damage after irradiation, but the damage was significantly alleviated after RvE1 pretreatment compared to irradiation alone. Morphological analysis of the inner ear showed severe damage to the cochlea and vestibule after irradiation. In contrast, damage to the cochlea and vestibule was significantly reduced in the RvE1-pretreated group compared to that in the irradiation alone group. Along with these functional and morphological changes, the mRNA expression level of anti-inflammatory factors interleukin-2 was significantly increased, while those of proinflammatory factors interleukin-6 and tumor necrosis factor-α were significantly decreased in the inner ear of mice after RvE1 pretreatment compared to irradiation alone. Conclusions: We believe that inflammation induced by irradiation is involved in the damage to the inner ear caused by radiotherapy, and that RvE1 reduces the damage caused by irradiation to the inner ear by regulating the induced inflammatory response.

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Section: Discussionmentioning

confidence: 99%

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

Zhang

Niu

et al. 2020

Preprint

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“…We previously reported that RvE1 can suppress the activation of DPFs to inhibit pulp in ammation, at least partially, in a ChemR23-dependent manner [17]. RvE1 reportedly has at least two receptors (ChemR23 and BLT1) [29].…”

Section: Discussionmentioning

confidence: 99%

“…All cavities were immediately restored with composite resin (Kerr Corp, Orange, CA, USA). The doses of RvE1 were based upon previous dose-response studies in rat [15,17,22]. On day 1, 3, 7, 14, 21, or 28 after the operation, the animals in the treated and non-treated (control) groups were sacri ced by intracardiac perfusion with 4% paraformaldehyde (PFA) buffered at pH 7.2-7.4.…”

Section: Pulp Injury Model Establishmentmentioning

confidence: 99%

“…In rat molar damaged pulp tissue, topical application of RvE1 limited in ammatory cell in ltration [15,16]. Our previous studies investigated the effects of RvE1 on dental pulp broblasts (DPFs) during the pathogenesis of pulpitis and proved its potential in inhibiting pulp in ammation and promoting resolution by suppressing DPF activation [17]. Moreover, dental-derived stem cells (e.g., periodontal ligament stem cells, stem cells from the apical papilla) have been found to produce and release SPMs, which generate a favorable microenvironment for dental stem cells to promote tissue self-repair [18,19].…”

Section: Introductionmentioning

confidence: 99%

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Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells

Chen

Xia

et al. 2020

Preprint

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Background Unresolved inflammation and tissue destruction are considered to underlie failure of dental pulp repair. As key mediators of the injury response, dental pulp stem cells (DPSCs) play a critical role in pulp tissue repair and regeneration. Resolvin E1 (RvE1), a major dietary omega-3 polyunsaturated fatty-acid metabolite, is effective in resolving inflammation and activating wound healing. However, whether RvE1 facilitates injured pulp-tissue repair and regeneration through timely resolution of inflammation and rapid mobilization of DPSCs is unknown. Therefore, we established a pulp injury model and investigated the effects of RvE1 on DPSC-mediated inflammation resolution and injured pulp repair. Methods A pulp injury model was established using eight-week-old Sprague-Dawley rats. Animals were sacrificed on days 1, 3, 7, 14, 21, and 28 after pulp capping with a collagen sponge immersed in PBS with RvE1 or PBS. Hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry, and immunohistofluorescence were used to evaluate the prohealing properties of RvE1. hDPSCs were incubated with lipopolysaccharide (LPS) to induce an inflammatory response, and the expression of inflammatory factors after RvE1 application was measured. Effects of RvE1 on hDPSC proliferation, chemotaxis, and odontogenic differentiation were evaluated by CCK-8 assay, transwell assay, alkaline phosphatase (ALP) staining, alizarin red staining, and quantitative PCR, and possible signaling pathways were explored using western blotting. Results In vivo, RvE1 promoted injured pulp repair and reparative dentin formation. Further, it enhanced dentin sialoprotein and nestin expression and accelerated pulp inflammation resolution by suppressing TNF-α and CD68 expression. RvE1 enhanced the recruitment of CD146+ and CD105+ DPSCs to the damaged molar pulp mesenchyme. Isolated primary cells exhibited the mesenchymal stem cell immunophenotype and differentiation. RvE1 promoted hDPSC proliferation and chemotaxis. RvE1 significantly attenuated pro-inflammatory cytokine (TNF-α, CD68, IL-6, and IL-1β) release and enhanced ALP activity, nodule mineralization, and especially, expression of the odontogenesis-related genes DMP-1, DSPP, and BSP in LPS-stimulated DPSCs. RvE1 regulated Akt, ERK, and rS6 phosphorylation. Conclusions RvE1 promotes pulp inflammation resolution and dentin regeneration and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. This response is, at least partially, dependent on Akt, ERK, and rS6-associated signaling. RvE1 has promising application potential in regenerative endodontics.

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“…The influence of RvE1 on these inflammatory factors has been previously reported. Xu et al reported that RvE1 ameliorates pulpitis by inhibiting the expression of the proinflammatory cytokines IL-6 and TNF-α in a chemerin receptor23 (ChemR23)-dependent manner [28]. Rey et al reported that RvE1 decreased LPS-induced gene expression of proinflammatory cytokines (TNF-α and IL-6), suggesting that they exert proresolutive activity in microglia [29].…”

Section: Discussionmentioning

confidence: 99%

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

Zhang

Niu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background In addition to the direct effects of irradiation, the induced inflammatory response may play an important role in the damage to the inner ear caused by radiotherapy for the treatment of head and neck cancers. Resolvin E1 has anti-inflammatory activity, acting by reducing neutrophil infiltration and proinflammatory cytokine expression. Therefore, in this study we sought to confirm whether the inflammation induced by irradiation was involved in damage to the inner ear after radiotherapy and to investigate the protective effect and underlying mechanism of resolvin E1 using mouse models.Methods A dose of resolvin E1 was delivered by intraperitoneal injection to mice before irradiation.Changes in the auditory brainstem response, relative balance ability, inner ear morphology and the expression levels of proinflammatory cytokines in the inner ear were analyzed on days 7 and 14 after irradiation and compared among three experimental groups.Results Morphological analysis of the inner ear showed severe damage to the cochlea and vestibule after irradiation. In contrast, damage to the cochlea and vestibule was significantly reduced in the resolvin E1-pretreated group, which nonetheless remained damaged compared to the wild-type group. Proinflammatory cytokines also showed expression patterns matching the morphological observations.Conclusions We believe that the inflammation induced by irradiation is involved in the damage to the inner ear caused by radiotherapy, and that resolvin E1 reduces the damage caused by irradiation to the inner ear by regulating the induced inflammatory response.Therefore in this study, a mouse experiment was designed to investigate these problems and the potential mechanism of RvE1 in protecting against irradiation-induced damage to the inner ear. Materials And Methods Animals and Study GroupsC57BL/6 mice weighing 25-27 grams were purchased from the Experimental Animal Center of Shandong University for this experiment, and all animal procedures in this study were approved by the Ethics Committee of the Second Hospital of Shandong University.Study Groups: 36 mice were randomly divided into three groups: a wild type group (WT), X-ray irradiation group (Rad), and RvE1-pretreated X-ray irradiation group (Rad+RvE1). On days 7 and 14 after irradiation, six mice in each group were sacrificed for experimental analysis. The irradiation groups were irradiated with 20 Gy in one fraction to the inner ear. This dose was determined according to the results of a preliminary experiment, to ensure robust survival of all experimental mice along with detectable damage to inner ear functions. Before irradiation, 10% chloral hydrate was used for enterocoelia anesthesia at a dose of 1,000 mg/kg without any signs of peritonitis after administration. On the day of the experiments, 1 μg RvE1 was administered to the mice via intraperitoneal injection 30 minutes before irradiation, according to a previous report [11,16].After the experimental mice were anesthetized and placed in a soundproof room, the collection ...

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Resolvin E1 Ameliorates Pulpitis by Suppressing Dental Pulp Fibroblast Activation in a Chemerin Receptor 23-dependent Manner

Cited by 31 publications

References 34 publications

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells

A unique radioprotective effect of resolvin E1 reduces irradiation-induced damage to the inner ear by inhibiting the inflammatory response

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