Introduction: Teachers stand out among occupational groups due to their contribution to modern societies, and their voice is the main form of interaction. Objective: To verify the changes in vocal and respiratory measurements from teachers with vocal and musculoskeletal complaints and with normal larynx, after applying a musculoskeletal manipulation protocol of myofascial release using pompage. Methods: Controlled and randomized clinical trial with 56 participants, 28 teachers in the study group and 28 teachers in the control group. Anamnesis; videolaryngoscopy; hearing screening; sound pressure and maximum phonation time measurements; and manovacuometry were performed. The musculoskeletal manipulation protocol of myofascial release using pompage consisted of a total of 24 sessions, 40 min each session, three times a week, for eight weeks. Results: There was a significant improvement in the maximum respiratory pressure in the study group after the intervention. The sound pressure level and the maximum phonation time did not change significantly. Discussion and Conclusion: Musculoskeletal manipulation protocol of myofascial release using pompage had a direct effect on the respiratory measurements from female teachers, significantly increasing the maximum respiratory pressure, but without affecting sound pressure level and the /a/ maximum phonation time.