Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses

Bazzano, Marilena; Laghi, Luca; Zhu, Chenglin; Magi, Gian Enrico; Tesei, Beniamino; Laus, Fulvio

doi:10.1186/s12917-020-02446-9

Cited by 12 publications

(9 citation statements)

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“…Due to quite invasive disease diagnostics, especially in severely asthmatic patients, there is a great need for biomarkers to facilitate a diagnosis based on blood analysis. Lately, researchers have also focused on exhaled breath gas and exhaled breath condensate (EBC) and its chemical and metabolomic analysis [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma

Słowikowska

Bajzert

Miller

et al. 2021

Animals

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Non-invasive diagnostic biomarkers of equine asthma syndrome (EAS) from blood or urine are sought. The aim of this study was to assess the absorbance of circulating immune complexes (CICs) during the exacerbation, remission, and treatment of an asthma episode and assess the potential usefulness of CIC levels in the diagnosis and monitoring of the disease. The control group, asthma group, and treated asthma group each contained six horses. Following an initial examination and group classification, the horses were kept in a dusty environment for seven days and then moved to an asthma-friendly environment for three weeks (the treated group received injections of glucocorticoids). Blood was collected at baseline and on the 1st, 2nd, 3rd, 7th, 14th and 30th days. CIC was measured using the modified Haskova method. The time points did not show significant statistical differences. There was a significant decrease in CIC in the treated group, and a significant increase in CIC in the non-treated group on day 30. CIC did not support the EAS diagnosis, although it may help in monitoring patients. To the best of the authors’ knowledge, this is the first study to analyse the dynamics of CIC during environmental challenge, remission, and treatment.

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Section: Introductionmentioning

confidence: 99%

The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma

Słowikowska

Bajzert

Miller

et al. 2021

Animals

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“…Up to now, no study about its effect in equine species has been performed. Recently, reduced concentrations of myo-inositol have been found in the bronchoalveolar lavage fluid of horses affected by equine asthma in comparison with healthy horses [56]. The exact role of this metabolite in physical exercise is not known, however, the decrease observed in both serum and saliva could indicate an impairment in the normal respiratory function during exercise.…”

Section: Discussionmentioning

confidence: 99%

Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

et al. 2020

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In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T0) and immediately after (T1) standardized exercise. Metabolomic analysis of both samples was performed by 1H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T0 and T1 in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.

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“…Several endotypes and phenotypes of equine asthma have even been recognized [ 189 , 191 ]. To enhance laboratory verification of equine asthma or to differentiate the asthma endotypes and phenotypes, several metabolomic studies have been recently published [ 86 , 192 , 193 ], demonstrating significant changes in lipid metabolites.…”

Section: Translational Value and Limitations Of Animal Models Of Asthmamentioning

confidence: 99%

Metabolomics in Animal Models of Bronchial Asthma and Its Translational Importance for Clinics

Barosova,

Baranovicova,

Hanusrichterova

et al. 2023

IJMS

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Bronchial asthma is an extremely heterogenous chronic respiratory disorder with several distinct endotypes and phenotypes. These subtypes differ not only in the pathophysiological changes and/or clinical features but also in their response to the treatment. Therefore, precise diagnostics represent a fundamental condition for effective therapy. In the diagnostic process, metabolomic approaches have been increasingly used, providing detailed information on the metabolic alterations associated with human asthma. Further information is brought by metabolomic analysis of samples obtained from animal models. This article summarizes the current knowledge on metabolomic changes in human and animal studies of asthma and reveals that alterations in lipid metabolism, amino acid metabolism, purine metabolism, glycolysis and the tricarboxylic acid cycle found in the animal studies resemble, to a large extent, the changes found in human patients with asthma. The findings indicate that, despite the limitations of animal modeling in asthma, pre-clinical testing and metabolomic analysis of animal samples may, together with metabolomic analysis of human samples, contribute to a novel way of personalized treatment of asthma patients.

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Respiratory metabolites in bronchoalveolar lavage fluid (BALF) and exhaled breath condensate (EBC) can differentiate horses affected by severe equine asthma from healthy horses

Cited by 12 publications

References 50 publications

The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma

The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma

Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

Metabolomics in Animal Models of Bronchial Asthma and Its Translational Importance for Clinics

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