Climate change, especially global warming, affects plant physiology and crop yields. Jajar Legowo (Jarwo) planting system, with the existing planting alleys that some farmers have applied, needs to be sufficiently identified for changes in microclimate factors, growth, and rice yield. This study aims to identify the microclimate factors, growth, and rice yield in this planting system. The research was designed through field experiments in rice fields with the Jarwo 2:1, 3:1, and 4:1 planting systems. The research variables included microclimate factors (temperature, humidity, and light intensity), rice growth, and yield. The results showed that the sunlight intercept in each Jarwo planting system was not different. The highest average temperature was recorded in the Jarwo 2:1 system and the lowest in the Jarwo 4:1. The highest average humidity was recorded in the Jarwo 4:1 system and the lowest in the Jarwo 2:1 system. The study's results noted no significant difference in the variables of plant height, total number of tillers, number of productive tillers, and grain weight per panicle. The observation of grain weight per clump Jarwo 3:1 is significantly different from Jarwo 4:1. The existence of alleys in the Jarwo planting system does not significantly affect plant growth and yield.