Response of Two Rosa sp. to Light Quality in Vitro.

Al-Rekaby, Layth Sareea

doi:10.24996/ijs.2023.64.10.15

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…For Al-Taif rose, a combination of blue + red light (1:2) was found to increase the root length and the number of roots while cool white + warm white (1:1) resulted in 100% rooting. Taken together, these results confirm previous findings in other rose species, including Rosa chinensis [43], Rosa canina [46], Rosa damascena, and Rosa hybrida [47].…”

Section: Effect Of Medium Salt Strength Sucrose Concentration and Lig...supporting

confidence: 92%

“…They found that an RB (i.e., 50% red and 50% blue) treatment significantly increased the number of roots per plant (i.e., 3.9). More recently, Al-Rekaby [47] pointed out that the highest values for several rooting parameters (i.e., root length, the number of roots, and fresh and dry root weight) for Rosa damascena and Rosa hybrida occurred when cultured under red light. Blue and red light are the most efficiently absorbed by chlorophyll during photosynthesis [48].…”

Section: Effect Of Medium Salt Strength Sucrose Concentration and Lig...mentioning

confidence: 99%

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Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization

Al-Ali,

Dewir,

Al-Obeed

2024

Agronomy

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Micropropagation facilitates the rapid production of roses. Nevertheless, in vitro rhizogenesis of essential oil roses has presented significant challenges, primarily attributed to low rates of rooting and poorer acclimatization compared to ornamental rose varieties. This study reports the optimization of in vitro rooting of Al-Taif rose (Rosa damascena f. trigintipetala (Diek) R. Keller) microshoots with the aim of increasing survival rate during acclimatization. We also investigated the effects of various parameters, including type and concentration of auxin (i.e., 2,4-Dichlorophenoxyacetic acid (2,4-D), indole acetic acid (IAA), indole butyric acid (IBA), and naphthaleneacetic acid (NAA) at concentrations of 0, 0.05, 0.1, 0.2, and 0.4 mg/L), salt strength (i.e., full- and half-strength Murashige and Skoog (MS) medium), sucrose concentration (i.e., 20, 30, 40, 60, and 80 g/L), light spectra (a 2:1 or 1:2 blue/red spectral ratio, cool or warm white light at a 1:1 ratio, and fluorescent light), light intensity (photosynthetic photon flux density (PPFD) values of 25, 50, and 100 µmol·m−2·s−1), and activated charcoal (i.e., 0 and 0.5 g/L) on the rooting and growth of in vitro regenerated Al-Taif rose axillary shoots. We found that half-strength MS medium supplemented with 0.2 mg/L NAA, 80 g/L sucrose, 0.5 g/L activated charcoal, and 50 μmol·m−2·s−1 PPFD were the optimal conditions for 100% induction of adventitious roots. Next, micropropagated Al-Taif rose plantlets were successfully transferred to a potting medium containing perlite/peatmoss (in a 1:1 ratio). We found that 98% of plants survived ex vitro conditions. The genetic fidelity of micropropagated Al-Taif rose clones along with their mother plant was tested using the inter-simple sequence repeats (ISSR) molecular marker. The genetic similarity between the micropropagated plantlets and the mother plant of Al-Taif rose plants was 98.8%, revealing high uniformity and true-to-type regenerated plants. These findings may therefore contribute toward the commercial micropropagation of Al-Taif roses.

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Section: Effect Of Medium Salt Strength Sucrose Concentration and Lig...supporting

confidence: 92%

Section: Effect Of Medium Salt Strength Sucrose Concentration and Lig...mentioning

confidence: 99%

Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization

Al-Ali,

Dewir,

Al-Obeed

2024

Agronomy

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Features of the clonal micropropagation technology of ornamental Rose varieties ‘Dream Come True’ and ‘Full Sail’

Makarov,

Kuznetsova,

Hussien

et al. 2024

Ornam. Hortic.

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The rose is the most important ornamental and floral crop, as well as a valuable aromatic and medicinal plant. For accelerated production of planting material, clonal micropropagation is used. There are problems in obtaining an aseptic culture and the peculiarities of plant cultivation at different stages, depending on the variety. It is necessary to develop and optimize the technological cycle of cultivation in in vitro culture. The results showed that the highest viability of rose explants obtained from lateral buds was noted by sterilization with 0.2% silver nitrate solution of and 5% Lysoformin 3000 with an exposure of 15 minutes (82%-95%); the highest viability of explants obtained from etiolated shoots was observed when sterilized with 0.2% silver nitrate solution with an exposure of 10 minutes and 5% Lysoformin 3000 with an exposure of 15 min (82%-100%). The maximum length of the micro-shoots was noted in the ‘Dream Come True’ variety on a QL (Quoirin and Lepoivre) nutrient medium (on average 21.2 cm), in the ‘Full Sail’ variety - on a ½ QL medium (on average 24.9 cm). An increase in the concentration of 6-BAP from 0.5 to 1.0 mg L-1 in the nutrient medium contributed to an increase in the number of micro-shoots (by 1.7-1.9 times). The maximum root length was noted on the nutrient medium ½ QL (on average 88.7-95.3 cm). An increase in the concentration of IAA from 0.5 to 1.0 mg L-1 in the nutrient medium contributed to an increase in root length by 1.4-1.8 times. The protocol developed in this study allows to propagate these two varieties in vitro and produce a large number of plants.

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Response of Two Rosa sp. to Light Quality in Vitro.

Cited by 2 publications

References 27 publications

Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization

Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization

Features of the clonal micropropagation technology of ornamental Rose varieties ‘Dream Come True’ and ‘Full Sail’

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