RESPONSE SURFACE OPTIMIZATION OF BIOSURFACTANT PRODUCED BYPseudomonas aeruginosaMA01 ISOLATED FROM SPOILED APPLES

Abstract

A potent biosurfactant-producing bacterial strain isolated from spoiled apples was identified by 16S rRNA as Pseudomonas aeruginosa MA01. Compositional analysis revealed that the extracted biosurfactant was composed of high percentages of lipid (66%, w/w) and carbohydrate (32%, w/w). The surface tension of pure water decreased gradually with increasing biosurfactant concentration to 32.5 mN m(-1) with critical micelle concentration (CMC) value of 10.1 mg L(-1). The Fourier transform infrared spectrum of extracted biosurfactant confirmed the glycolipid nature of this natural product. Response surface methodology (RSM) was employed to optimize the biosynthesis medium for the production of MA01 biosurfactant. Nineteen carbon sources and 11 nitrogen sources were examined, with soybean oil and sodium nitrate being the most effective carbon and nitrogen sources on biosurfactant production, respectively. Among the organic nitrogen sources examined, yeast extract was necessary as a complementary nitrogen source for high production yield. Biosurfactant production at the optimum value of fermentation processing factor (15.68 g/L) was 29.5% higher than the biosurfactant concentration obtained before the RSM optimization (12.1 g/L). A central composite design algorithm was used to optimize the levels of key medium components, and it was concluded that two stages of optimization using RSM could increase biosurfactant production by 1.46 times, as compared to the values obtained before optimization.

Statistical Design, a Powerful Tool for Optimizing Biosurfactant Production: A Review

“…Another very interesting study was carried out by Abbasi et al [3]. Their strategy for finding the optimum conditions for maximum Bs production by P. aeruginosa was complete.…”

“…Shaking has also been shown to affect Bs production. The greater oxygenation at higher shaking speed is related to higher metabolism rates [3,29].…”

“…They attract interest for application in diverse industrial areas including the cosmetic, pharmaceutical, agricultural, food, and petrochemical industries, because of their biochemical properties [1][2][3]. In fact, the global biosurfactant market is expected to reach 2.2 billion US dollars this year, based on a growth rate of 3.5% per annum.…”

Glycolipid biosurfactants: Potential related biomedical and biotechnological applications

“…In addition to these, the production efficiency of glycolipids using microorganisms has been improved along with the progress of Page 6 of 46 biotechnology by the amelioration of fermentation conditions 45 , the application of solid state fermentation process [83][84][85] and the optimization of production by means of the response surface methodology. 86,87 Generally, the yields of bioconversion are always so high that they permitted the retention of huge quantity of glycolipid biosurfactants for diverse applications.…”

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

“…Glycolipid biosurfactants can be produced from inexpensive raw materials that are available in large quantities, such as industrial wastes and oily byproducts, including hydrocarbons, frying oil waste and olive oil waste. In addition to these, the production efficiency of glycolipids using microorganisms has been improved alongside progress in biotechnology as a result of the amelioration of fermentation conditions, the application of the solid state fermentation process and the optimization of production by means of response surface methodology . Generally, the yields of bioconversion are always so high that they permit the retention of huge quantity of glycolipid biosurfactants for diverse applications.…”