Response surface optimization of enzymatic hydrolysis of duck blood corpuscle using commercial proteases

Abstract

With the rapid development in livestock and poultry husbandry and increasing shortage of protein sources, recycling of wastes from agricultural and food processing such as blood corpuscles has been regarded as an important industrial procedure to obtain protein sources. This study aimed to find an appropriate method for recycling the considerable amounts of blood corpuscle so as to improve its nutritional value and organoleptic quality. An effective production process for enzymatic hydrolysis of duck blood corpuscle was successfully developed and optimized by response surface methodology. Optimal conditions based on achieving a high value of trichloroacetic acid solubility index were substrate concentration of 14 g/100 mL, temperature 51°C, initial pH 7.0, and time 7.5 h. The electrophoretic patterns of the protein hydrolysate were investigated, and a large diffuse band was observed in the vicinity of 5 kDa. The organoleptic quality of spray-dried blood corpuscle hydrolysate was also evaluated, indicating that enzymatic hydrolysis and decoloration methods were feasible and cost-effective to achieve the desirable bright yellow product without bitterness. In vitro protein digestibility of blood corpuscle hydrolysate was 96.32 ± 0.50%, which was better than that of soybean, fish meal, and casein. Based on the amino acid composition and nutritional parameters, we found that the spray-dried blood corpuscle hydrolysate had abundant nutritional value and high potential for application as an ingredient in nonruminant animal feed.

Comparative assessment of physicochemical and antioxidative properties of mung bean protein hydrolysates

“…Nevertheless, both ficin and bromelain caused the obvious downward shifts to increased percentages of the small peptides (<10 kDa), indicating the bioactive properties of mung bean protein hydrolysates. 27 …”

“…Nevertheless, both cin and bromelain caused the obvious downward shis to increased percentages of the small peptides (<10 kDa), indicating the bioactive properties of mung bean protein hydrolysates. 27 3.3 Structural characterization of mung bean protein hydrolysates 3.3.1 Ultraviolet-visible spectra analysis. Ultraviolet-visible spectroscopy assay was conducted to investigate the absorption spectra of hydrolysates obtained with cin and bromelain.…”

Investigation of optimal conditions for production of antioxidant peptides from duck blood plasma: response surface methodology

“…The protein content was lower than that (89.7%) of whole duck blood powder ( Sorapukdee and Narunatsopanon, 2017 ). On the other hand, the protein content of DBP was higher than that of duck blood corpuscle powder (38.74%) ( Zheng et al., 2014 ). Thus, DBP with high protein content and low lipid is suitable for preparing bioactive peptides.…”

In ovo feeding of nutrients and its impact on post‐hatching water and feed deprivation up to 48 hr, energy status and jejunal morphology of chicks using response surface models

“…Kurt and Kilincceker () and Zheng et al. () indicated that the RSM is an important mathematical and statistical technique for optimising the interactions of factors and factor levels and reduce research and production costs.…”

“…Therefore, knowledge of the total experimental system increases (Myers & T A B L E 9 Estimated parameters of second-order response surface models for glycogen content of liver and breast of chicks at day 2 Montgomery, 2009). Kurt and Kilincceker (2011) and Zheng et al (2014) indicated that the RSM is an important mathematical and statistical technique for optimising the interactions of factors and factor levels and reduce research and production costs.…”