Response surface optimization of polysaccharide extraction from Galla Chinensis and determination of its antioxidant activity in vitro

Abstract

Galla Chinensis was a traditional Chinese medicine used as an ingredient in many prescriptions for the treatment of oxidant damage-associated symptoms in China. The current research on the isolation of polysaccharides from Galla Chinensis and their antioxidant activities still remain as an ongoing challenge. The purpose of this study is to develop an isolation technique for polysaccharides from Galla Chinensis and determine their antioxidant properties in vitro. An ultrasonic-assisted method of polysaccharides extraction from Galla Chinensis were firstly developed and the key parameters were optimized response surface analysis as crushing degree 100 mesh, ultrasonic time 20 min, and materials/liquid ratio 1: 40. The polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in DPPH, ABTS, total antioxidant and Iron ion reduction assays. Moreover, it also demonstrated an effective protective effect for human under H 2 O 2 -induced oxidative damage on human embryonic kidneyfibroblast 293 and hepatoma 7721 cells by cell proliferation assay. The study firstly determined the antioxidant activities of polysaccharides from Galla Chinensis, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products or addictives.

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

“…Similarly, Hu et al reported a maximum activity of 0.25 at 350 µg/mL for polysaccharides extracted by ultrasound from Galla chinensis at 60 °C, 20 min, and 40 mL/g [ 40 ].…”

“…Zhang et al reported that the polysaccharides extracted using ultrasound exhibit lower metal chelation power than those by maceration. The maximum chelation activity is 38% for ultrasound and 86% for maceration at 5 mg/mL [ 40 ]. Similar findings were reported by Surin et al The authors obtained an IC 50 of 1.25 and 3.7 mg/mL for polysaccharides extracted by maceration and by ultrasound, respectively [ 50 ].…”

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“…Dry fruiting bodies powder (400 g) was boiled in water for 3 times (6 h for each) (Hu et al, 2021;. The supernatant was concentrated after centrifugation.…”