Response to Cardiac Markers in Human Serum Analyzed by Guided-Mode Resonance Biosensor

Abstract

Cardiac markers in human serum with concentrations less than 0.1 ng/mL were analyzed by use of a guided-mode resonance (GMR) biosensor. Cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin (MYO) were monitored in the serum of both patients and healthy controls. Dose-response curves ranging from 0.05 to 10 ng/mL for cTnI, from 0.1 to 10 ng/mL for CK-MB, and from 0.03 to 1.7 μg/mL for MYO were obtained. The limits of detection (LOD) for cTnI, CK-MB, and MYO were less than 0.05, 0.1, and 35 ng/mL, respectively. Analysis time was 30 min, which is short enough to meet clinical requirements. Antibody immobilization and the hydrophilic properties of the guided-mode resonance filter (GMRF) surface were investigated by X-ray photoelectron spectroscopy (XPS) and by monitoring the peak wavelength shift and water contact angle (CA). Both assays used to evaluate the surface density of the immobilized antibodies, a sandwich enzyme-linked immunosorbent assay (ELISA) and a sandwich immunogold assay, showed that the antibodies were successfully immobilized and sufficiently aligned to detect the low concentration of biomarkers. Our results show that the GMR biosensor will be very useful in developing low-cost portable biosensors that can screen for cardiac diseases.

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

“…193 The dose−response ranges and LODs for cTnI, CK-MB, and MYO were 0.05−10 ng mL −1 and 0.05 ng mL −1 , 0.1−10 ng mL −1 and 0.1 ng mL −1 , and 0.03−1.7 μgmL −1 and 35 ng mL An all-fiber optofluidics-based bioassay platform (AFOB) has been attempted for the highly sensitive, selective, and rapid Optical biosensors have also been designed to detect whole microorganisms rather than just small molecules such as proteins. The detection of pathogenic microorganisms is critical for controlling foodborne illnesses.…”

A Compact Tunable VCSEL and a Built-in Wavelength Meter for a Portable Optical Resonant Reflection Biosensor Reader

“…By measuring the resonance peak shift (Δλ), the existence of a specific antigen can be determined, and the concentration of any antigens existing in the sample can be measured. Typically, the amount of the peak wavelength red-shift caused by the antigen-antibody interaction is 0.1~1.0 nm for immunoglobulin (IgG), cTnI, CK-MB, myoglobin [15,16]. Fig.…”

“…Because the peak wavelength shift of a general GMRF by antigen-antibody interaction is 0.1~1.0 nm for biomarker concentrations of 1 pg/ml ~ 1 ug/ml [15,16], a wavelength meter with high stability (<0.02 nm) will be used for biosensor applications.…”

Portable guided-mode resonance biosensor platform for point-of-care testing

“…GMRF can be fabricated by coating high refractive index materials such as SiN x and TiO 2 onto either polymer grating imprinted on a glass substrate [9] or injection molded polymer grating structure [10]. The injection molding method provides effective way to the mass production with improved performance of the device and very low cost.…”