Response to the Letter to the Editor regarding ‘Human dirofilariosis in the 21st century: A scoping review of clinical cases reported in the literature’ by Simón et al. (Transboundary and Emerging Diseases; 2021: http://doi.org/10.1111/tbed.14210)

Simón, F; Diosdado, Alicia; Siles-Lucas, Mar; Kartashev, Vladimir; González‐Miguel, Javier

doi:10.1111/tbed.14430

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Similar data have been published by scientists from China [25]. While a number of scientists write about the presence of 13 different types of cryptosporidia in piglets [10][11][12][13], However, the dominant species worldwide are still such as C. suis and C. scrofarum [14,10].…”

Section: Resultssupporting

confidence: 59%

“…Thirteen different Cryptosporidium species/genotypes were isolated from pigs, namely Cryptosporidium scrofarum (formerly Cryptosporidium, pig genotype II), Cryptosporidium suis (formerly Cryptosporidium, pig genotype I), Cryptosporidium muris, Cryptosporidium parvum, Cryptosporidium tyzzeri (formerly mouse genotype I Cryptosporidium ), Cryptosporidium hominis, Cryptosporidium meleagridis, Cryptosporidium felis, Cryptosporidium andersoni, Cryptosporidium struthioni, rat Cryptosporidium genotype, Cryptosporidium sp. Eire w65.5 genotype and unknown Cryptosporidium genotype from pig manure [10][11][12][13]. More than 90% of cases of cryptosporidiosis in pigs are caused by Cryptosporidium suis and Cryptosporidium scrofarum species [14], and the potential danger of human infection with them is also reported [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Features of prevalence of ASV types of Cryptosporidium scrofarum in pig farms in the Northwestern of Russia

Kryazhev,

Novikov

2024

BIO Web Conf.

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For the first time in the Russian Federation, using the example of the Vologda region of the North-West of the Russian Federation in pig farms by high-performance sequencing of amplicon libraries of fragments of the 18S rRNA gene obtained as a result of nested PCR, we have established parasitization of Cryptosporidium scrofarum in pigs of all age groups. The infection rate of animals kept in pig farms was 34%, in farms – 32.4%. Piglets that are fattening at the age of 13-24 weeks are most susceptible to infection. The analysis of the taxonomic affiliation of ASV carried out using phylogenetic analysis, supplemented by analysis using the blastn algorithm in the GenBank database, showed that in total, 10 ASV types (amplicon sequence variant) with high similarity to sequences deposited in GenBank as fragments of the Cryptosporidium scrofarum 18S rRNA gene are present in all the samples studied. It was found that the ASV1 and ASV2 types detected in various geographical regions of the world from Portugal and Great Britain to China, India and Australia were identified in all surveyed farms, although in significantly different quantities. The remaining ASVS belong to local populations of C. scrofarum subspecies. A unique sequence of the genus Cryptosporidium of type ASV 8 has been discovered, which can later be described as a new species. The nucleotide sequences we have discovered are unique. Each of them was deposited in GenBank with the assignment of identifiers (Sequence ID: OR649139, OR654022, OR654023, OR661243, OR661244, OR654051, OR654052, OR654083, OR654084, OR654106).

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Section: Resultssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Features of prevalence of ASV types of Cryptosporidium scrofarum in pig farms in the Northwestern of Russia

Kryazhev,

Novikov

2024

BIO Web Conf.

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Zoonotic Dirofilaria sp. “hongkongensis” in subcutaneous nodules from dogs and cats, Hong Kong SAR

Manathunga,

Tse,

Perles

et al. 2024

Parasites Vectors

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Background Dirofilaria sp. “hongkongensis” is a putative Dirofilaria species, initially identified in subcutaneous nodules in humans in Hong Kong and in other South and Southeast Asian regions. While it differs genetically from the better-known zoonotic species, Dirofilaria repens and Dirofilaria immitis, information on the lesions caused by Dirofilaria sp. “hongkongensis” in the hosts as well as on its biology is scarce. This study documents for the first time the presence of this filarioid nematode in subcutaneous nodules in dogs and cats in Hong Kong, where it was originally described in human patients, therefore providing evidence for the zoonotic nature of this parasite. Methods Records of Veterinary Diagnostic Laboratory of City University of Hong Kong were searched between 2019 and 2024 for histological reports of possible filarioid-associated lesions. Tissue samples were collected by excisional surgical biopsy and processed with routine paraffin techniques. Selected slides were stained using various staining techniques [i.e., hematoxylin and eosin, periodic acid–Schiff (PAS), Grocott methenamine silver (GMS) or Ziehl–Neelsen (ZN) and Gram stain]. DNA from formalin-fixed paraffin-embedded tissue were extracted, submitted to conventional polymerase chain reaction (cPCR) and sequencing (i.e., cox1 and 12S rRNA genes) and phylogenetic analyzed. Results A total of five subcutaneous nodules from four cats and one from a dog with histopathology suggestive of filariosis were selected. The presence of Dirofilaria sp. “hongkongensis” was morphologically and molecularly confirmed in one dog and one cat. Both histopathological presentation and phylogenetic analysis enabled classification of this species close to D. repens and within the subgenus Nochtiella. In the remaining three cases, one showed histological evidence of aberrant nematode migration, while non-parasitic causes were identified in the other two. Conclusions This study provides the first evidence of Dirofilaria sp. “hongkongensis” in subcutaneous nodules in cats and dogs. The histology of clinical lesions of this filarioid species herein described is closely related to those caused by D. repens. Overall, this species should be considered in differential diagnoses of subcutaneous lesions in both animals and humans in the region. Graphical Abstract

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Response to the Letter to the Editor regarding ‘Human dirofilariosis in the 21st century: A scoping review of clinical cases reported in the literature’ by Simón et al. (Transboundary and Emerging Diseases; 2021: http://doi.org/10.1111/tbed.14210)

Cited by 2 publications

References 11 publications

Features of prevalence of ASV types of Cryptosporidium scrofarum in pig farms in the Northwestern of Russia

Features of prevalence of ASV types of Cryptosporidium scrofarum in pig farms in the Northwestern of Russia

Zoonotic Dirofilaria sp. “hongkongensis” in subcutaneous nodules from dogs and cats, Hong Kong SAR

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