Responses of
            <i>Listeria monocytogenes</i>
            to Acid Stress and Glucose Availability Revealed by a Novel Combination of Fluorescence Microscopy and Microelectrode Ion-Selective Techniques

Shabala, Lana; Budde, Birgitte Bjørn; Ross, Tom; Siegumfeldt, Henrik; Jakobsen, Mogens; McMeekin, TA

doi:10.1128/aem.68.4.1794-1802.2002

Cited by 47 publications

(47 citation statements)

References 27 publications

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“…3E). This result was consistent with the previous finding that an instantaneous H ϩ influx leads to a decrease in the pHi (24). However, the pHi values of all three strains were significantly different.…”

Section: Resultssupporting

confidence: 93%

Acetylation Regulates Survival of Salmonella enterica Serovar Typhimurium under Acid Stress

Ren

Sang

et al. 2015

Appl Environ Microbiol

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The ability to acetylate lysine residues is conserved across organisms, and acetylation of lysine residues plays important roles in various cellular functions. Maintaining intracellular pH homeostasis is crucial for the survival of enteric bacteria in the acidic gastric tract. It has been shown that eukaryotes can stabilize the intracellular pH by histone deacetylation. However, it remains unknown whether bacteria can utilize a reversible protein acetylation system to adapt to an acidic environment. Here we demonstrate that protein acetylation/deacetylation is critical for Salmonella enterica serovar Typhimurium to survive in an acidic environment. We used RNA sequencing to analyze the transcriptome patterns under acid stress and found that the transcriptional levels of genes involved in NAD ؉ /NADH metabolism were significantly changed, leading to an increase in the intracellular NAD ؉ /NADH ratio. Moreover, acid stress downregulated the transcriptional level of pat, encoding acetyltransferase, and genes cyaA and crp, encoding adenylate cyclase and cyclic AMP receptor protein, respectively, which are positive regulators of pat. It was found that the acid signal alerts the tricarboxylic acid cycle to promote the consumption of acetyl coenzyme A (Ac-CoA), an acetyl group donor for the acetylation reaction. A lowered acetylation level not only was the bacterial response to acid stress but also increased the survival rate of S. Typhimurium under acid stress. The pat deletion mutant had a more stable intracellular pH, which paralleled the higher survival rate after acid treatment compared with that of both the wild-type strain and the cobB (encoding deacetylase) deletion mutant. Our data indicate that bacteria can downregulate the protein acetylation level to prevent the intracellular pH from further falling under acid stress, and this work may provide a new perspective to understand the bacterial acid resistance mechanism.

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Section: Resultssupporting

confidence: 93%

Acetylation Regulates Survival of Salmonella enterica Serovar Typhimurium under Acid Stress

Ren

Sang

et al. 2015

Appl Environ Microbiol

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“…This suggests that the changes in bioenergetics imposed by the (Fig. 1) agrees with previous work (20,23,42), although when HCl is used as the acidulant, pH i can be maintained above 7 at pH o as low as 4 in the presence of glucose (34). Clearly, the nature of the acidulant and the availability of an energy source to drive proton pumping are important considerations.…”

Section: Results Ph I In Atrsupporting

confidence: 89%

Bioenergetic Mechanism for Nisin Resistance, Induced by the Acid Tolerance Response of Listeria monocytogenes

Bonnet

Rafi

Chikindas

et al. 2006

Appl Environ Microbiol

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“…However, in the absence of glucose, a pH o of 5.5 causes a drop in pH i . In both cases, a drop in pH i coincides with a net influx of protons, indicating that membrane transport processes are the main contributors to the process of pH i homeostasis in L. monocytogenes subjected to acid stress (206).…”

Section: Listeria Monocytogenesmentioning

confidence: 91%

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Cotter

Hill

2003

Microbiol Mol Biol Rev

1,043

828

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Gram-positive bacteria possess a myriad of acid resistance systems that can help them to overcome the challenge posed by different acidic environments. In this review the most common mechanisms are described: i.e., the use of proton pumps, the protection or repair of macromolecules, cell membrane changes, production of alkali, induction of pathways by transcriptional regulators, alteration of metabolism, and the role of cell density and cell signaling. We also discuss the reponses of Listeria monocytogenes, Rhodococcus, Mycobacterium, Clostridium perfringens, Staphylococcus aureus, Bacillus cereus, oral streptococci, and lactic acid bacteria to acidic environments and outline ways in which this knowledge has been or may be used to either aid or prevent bacterial survival in low-pH environments

show abstract

Responses of Listeria monocytogenes to Acid Stress and Glucose Availability Revealed by a Novel Combination of Fluorescence Microscopy and Microelectrode Ion-Selective Techniques

Cited by 47 publications

References 27 publications

Acetylation Regulates Survival of Salmonella enterica Serovar Typhimurium under Acid Stress

Acetylation Regulates Survival of Salmonella enterica Serovar Typhimurium under Acid Stress

Bioenergetic Mechanism for Nisin Resistance, Induced by the Acid Tolerance Response of Listeria monocytogenes

Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

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