Responsiveness to basement membrane extract as a possible trait for tumorigenicity characterization

Murata, Haruhiko; Omeir, Romelda; Tu, Wei; Lanning, Lynda L.; Phy, Kathryn; Foseh, Gideon; Lewis, Andrew M.; Peden, Keith

doi:10.1016/j.jvacx.2019.100004

Cited by 2 publications

(5 citation statements)

References 23 publications

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“…Studies found that laminin-111 derived peptide YIGSR increased adhesion, migration, and decreased tumor growth, metastasis (as per a study) shown in Table 2B and invasion 9,10,23,24 . YIGSR binds to receptor 32/67 kD, which is specific for tumor cells, and hence this also serves as a potential target for cancer treatment.…”

Section: Fig 4: Targeted Therapeutic Delivery In Malignancy Using Lam...mentioning

confidence: 57%

“…AG73-liposome encapsulating diagnostic ultrasound contrast gas helped in contrast imaging to detect cancer lesions 8 . YIGSR blocked experimental metastasis formation in the lungs, prevented xenograft growth and angiogenesis 9,10 . YIGSR reduced the number of blood vessels in-vivo tumor growth 2 .…”

Section: Fig 3: Development Of the Cell-specific Targeting System Lam...mentioning

confidence: 96%

“…The YIGSR has been found to block xenograft growth, experimental metastasis formation in the lungs (intravenous injection). This also blocks bone (intracardiac injection) growth and angiogenesis 9,10 . As shown in Table 2A, YIGSR also blocked angiogenesis in several assays, including the in vitro tube formation, rabbit eye pocket assays, and chick chorioallantoic membrane (CAM) 10 .…”

Section: Fig 4: Targeted Therapeutic Delivery In Malignancy Using Lam...mentioning

confidence: 99%

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Tissue specificity is the essential need of the hour to cut down clinical abnormalities. Allowing desired drug concentration to reach the target site is the current trend of the drug delivery system. Laminin is an active component of the basal lamina and present in most cells and organs has shown great utility in this case. It is a heterotrimeric glycoprotein containing an α-chain, a β-chain, and a γ-chain and found in different isoforms. Laminin influences cell differentiation, migration, adhesion, neurite outgrowth, and angiogenesis. The active peptides screened from the whole-length laminin have been incorporated to develop targeted or tissue-specific approaches. This is because full-length laminin has many active sites and triggers various downstream signaling and functions. Laminin-derived peptides along with various carriers or vectors ensure specific binding to target tissues. The particular therapeutic or diagnostic agent is successfully targeted to the diseased site because of the affinity of peptide sequence towards the cell membrane receptors. Promising applications of laminin-derived peptides are observed in diagnostics, therapy, chemotherapy, and gene therapy. It helps in tumor imaging, delivers cancer therapeutics, and serves as a biomarker. Implant surfaces coated with laminin-derived peptides enhance attachment and biocompatibility and decreases peri-implant inflammation. Having able to influence angiogenesis, it has been found to serve the purpose in the tissue healing process too. Many other effective applications of laminin-derived peptides might be developed with advancing days, but to date, it accounts as one of the promising theranostic approaches.

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Section: Fig 4: Targeted Therapeutic Delivery In Malignancy Using Lam...mentioning

confidence: 57%

Section: Fig 3: Development Of the Cell-specific Targeting System Lam...mentioning

confidence: 96%

Section: Fig 4: Targeted Therapeutic Delivery In Malignancy Using Lam...mentioning

confidence: 99%

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2022

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“…Trypsinized cells are placed in a culture vessel with a magnetic stirrer inhibiting cell attachment to the substrate and improve cell-cell adhesion. 141,146 Alternatively, spheroids are grown in a hanging drop deposited on a microplate lid. The cells start to aggregate due to the gravity.…”

Section: Techniques For Generating 3d Cell Culturementioning

confidence: 99%

“…172,173 They often rely on artificial ECM, like Matrigel or BME-2, to promote their selforganization. 139,141 Organoids have the capacity to grow under an in vivo condition owning to their self-renewal/self-assemble capacity. When given a scaffolding extracellular environment, they form a 3D structure that presents a similar organization and functionality as an organ.…”

Section: Organoidsmentioning

confidence: 99%

Compaction and spheroid formation modulates stemness and differentiation of human pancreas organoids

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The incidence of diabetes type 1 (T1D) in children and young adults is increasing worldwide. T1D is well treated by insulin administration. However, there is currently no long-lasting cure for this ailment. The success rate of pancreatic islet transplantation to treat T1D is limited by the availability of patient-matched islets and the necessity of using life-long immunosuppressive medication. The difficulties caused by transplantation can be overcome by generating bio-engineered pancreatic islets from patient-derived progenitor cells. Aim of this thesis is to establish new strategies for the generation and analysis of pancreatic lineages derived from human progenitor cells. It reports on the optimization of a technique to form human pancreatic spheroids from hollow monolayered human pancreas organoids (hPOs) to investigate how cell-cell and cell-matrix interaction can be leveraged to induce endocrine differentiation of the pancreas progenitor cell organoids. We introduce cell aggregation protocols to generate endocrine pancreas cell lineages from ductal pancreatic cells. Next, we study the effect of co-culture with stromal and endothelial cells to promote cell differentiation toward a pancreatic fate enhancing β cells productivity. This thesis has focused on identifying the differences in gene expression along with phenotypical transformation during differentiation of human pancreatic organoids (hPOs) towards human β cells to be used in the future of cellular therapeutics in treating T1D patients.

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Responsiveness to basement membrane extract as a possible trait for tumorigenicity characterization

Cited by 2 publications

References 23 publications

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Compaction and spheroid formation modulates stemness and differentiation of human pancreas organoids

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