Interruptions are a part of our everyday lives. They are inevitable in complex societies, especially when many people move from one place to another as a part of their daily routines. The main aim of this research is to understand the effects of interruptions on individuals from a psychological and crowd dynamics perspective. Two studies were conducted to investigate this issue, with each focusing on different types of interruptions and examining their psychological (emotion, motivation, arousal) and physiological (heart rate) components. Study 1 examined interruptions in a video game setting and systematically varied goal proximity (N = 61). It was hypothesized that being interrupted in the later stages of goal pursuit would create a high aroused impatience state, while interruptions in the earlier stages would produce a low aroused boredom state. However, the results showed that the hypothesized groupwise differences were not observed. Instead, interruptions created annoyance in all conditions, both psychologically and physiologically. Study 2 investigated interruptions in pedestrian crowds (N = 301) and used a basic motivational dichotomy of high and low motivation. In the experiments, crowds (80–100 participants) were asked to imagine that they were entering a concert hall consisting of a narrow bottleneck. The low motivation group reported feeling bored during the interruption, while the high motivation group reported feeling impatient. Additionally, a motivational decrease was observed for the high motivation group due to the interruption. This drop in motivation after the interruption is also reflected in the measured density (person/m2) in front of the bottleneck. Overall, both studies showed that interruption can have significant effects on individuals, including psychological and physiological impacts. The observed motivational decrease through interruption is particularly relevant for crowd management, but further investigation is needed to understand the context-specific effects of interruptions.