Restoration of cell growth and proliferation in the wheat roots after their inhibition by nickel sulfate

Demchenko, N. P.; Kalimova, I. B.; Demchenko, Kirill N.

doi:10.1134/s1021443713050026

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…Root tips are the most active region in root cell elongation, and they respond directly to environmental stress . The standard root elongation bioassay measures the longest root of each plant, based on the assumption that its length correlates with metal toxicity.…”

Section: Discussionmentioning

confidence: 99%

Terrestrial toxicity model for nickel: Comparison of culture method and modeling approaches

Jiang

Zhao

et al. 2018

Enviro Toxic and Chemistry

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In metal toxicity model studies of soil-plant systems, hydroponic culture is often employed to simulate soil conditions, but the validity of this approach has not been examined. The present study evaluated Ni toxicity to wheat root elongation using models developed in hydroponic and sand cultures and then compared the results with those obtained from real soils. Three mechanism toxicity models were examined: the free ion activity model, the biotic ligand model (BLM), and the Gouy-Chapman-Stern model. After being developed in hydroponic and sand culture bioassays, the models were validated with 18 soils from across China. The results showed that more accurate predictions were obtained with sand rather than hydroponic bioassays. Dissolved organic matter exerted only a limited effect on Ni toxicity, whereas an important role was found for osmotic effects. Of the 3 models, the sand-based BLM best predicted Ni toxicity to wheat root elongation (root-mean-square error = 16.2% and R = 0.79) and was as good as the BLM estimated directly from soils. The present study shows that sand is a better matrix than solution for root elongation bioassays of Ni toxicity and that osmotic effects must be taken into account in evaluating soil conditions. Environ Toxicol Chem 2018;37:1349-1358. © 2018 SETAC.

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Section: Discussionmentioning

confidence: 99%

Terrestrial toxicity model for nickel: Comparison of culture method and modeling approaches

Jiang

Zhao

et al. 2018

Enviro Toxic and Chemistry

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“…Root elongation comes from the 2 driving forces of cell mitosis and cell elongation in the direction of the longitudinal axis . The region in which root hair grows is considered to be the most active part for cell elongation , and it is the region in which root growth responds to environmental variation or toxicity . The conventional understanding is that root hairs increase root surface area, thereby enhancing metal uptake, which then results in increased toxicity and lower EC50 .…”

Section: Discussionmentioning

confidence: 99%

Barley root hair growth and morphology in soil, sand, and water solution media and relationship with nickel toxicity

Yanqing

Allen

Toro

2016

Enviro Toxic and Chemistry

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Barley, Hordeum vulgare (Doyce), was grown in the 3 media of soil, hydroponic sand solution (sand), and hydroponic water solution (water) culture at the same environmental conditions for 4 d. Barley roots were scanned, and root morphology was analyzed. Plants grown in the 3 media had different root morphology and nickel (Ni) toxicity response. Root elongations and total root lengths followed the sequence soil > sand > water. Plants grown in water culture were more sensitive to Ni toxicity and had greater root hair length than those from soil and sand cultures, which increased root surface area. The unit root surface area as root surface area per centimeter of length of root followed the sequence water > sand > soil and was found to be related with root elongation. Including the unit root surface area, the difference in root elongation and 50% effective concentration were diminished, and percentage of root elongations can be improved with a root mean square error approximately 10% for plants grown in different media. Because the unit root surface area of plants in sand culture is closer to that in soil culture, the sand culture method, not water culture, is recommended for toxicity parameter estimation. Environ Toxicol Chem 2016;35:2125-2133. © 2016 SETAC.

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“…This may simplify the formation of the mitotic spindle and speed up cell division. Until now, the knowledge about mitotic dynamics and the proportion of cells in different cell cycle stages in Triticeae is based on the combination of replication‐coupled chromosome labeling with modified nucleotides and flow sorting which enabled to set length of the different cell cycle stages (Baiza et al., 1989; Demchenko & Demchenko, 2001; Mickelson‐Young et al., 2016). The flow sorting technique of interphase nuclei or metaphase chromosomes in combination with fluorescence in situ hybridization (FISH) and immunostaining extended knowledge about their structure and organization (Fuchs et al., 2006; Jasencakova et al., 2001).…”

Section: Introductionmentioning

confidence: 99%

Spatial organization and dynamics of chromosomes and microtubules during barley mitosis

et al. 2023

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SUMMARY Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers and allow plant growth and development. Here, we analyzed the organization and dynamics of mitotic chromosomes, nucleoli, and microtubules in living cells of barley root primary meristems using a series of newly developed stable fluorescent protein translational fusion lines and time‐lapse confocal microscopy. The median duration of mitosis from prophase until the end of telophase was 65.2 and 78.2 min until the end of cytokinesis. We showed that barley chromosomes frequently start condensation before mitotic pre‐prophase as defined by the organization of microtubules and maintain it even after entering into the new interphase. Furthermore, we found that the process of chromosome condensation does not finish at metaphase, but gradually continues until the end of mitosis. In summary, our study features resources for in vivo analysis of barley nuclei and chromosomes and their dynamics during mitotic cell cycle.

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Restoration of cell growth and proliferation in the wheat roots after their inhibition by nickel sulfate

Cited by 5 publications

References 18 publications

Terrestrial toxicity model for nickel: Comparison of culture method and modeling approaches

Terrestrial toxicity model for nickel: Comparison of culture method and modeling approaches

Barley root hair growth and morphology in soil, sand, and water solution media and relationship with nickel toxicity

Spatial organization and dynamics of chromosomes and microtubules during barley mitosis

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