Restoring Vocal Fold Movement after Transection and Immediate Suturing of the Recurrent Laryngeal Nerve with Local Application of Basic Fibroblast Growth Factor: An Experimental Study in the Rat

Motoyoshi, Kazumi; Hyodo, Masamitsu; Yamagata, Takahiko; Gyo, Kiyofumi

doi:10.1097/00005537-200407000-00020

Cited by 24 publications

(12 citation statements)

References 29 publications

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“…Muscle fibre size is therefore a useful parameter to record the effect of growth factors. Both basic fibroblast growth factor (Motoyoshi et al ., 2004) and insulin‐like growth factor (Shiotani et al ., 1998) have been shown to enhance the cross‐sectional area of the TA muscle following RLN transection. However, we found that NT‐3 had no overall effect on PCA muscle fibre size, possibly because the NT‐3 enhanced axonal growth was not translated into sufficiently increased muscle reinnervation or the muscle fibres were reinnervated by an ‘incorrect’ neuronal type.…”

Section: Discussionmentioning

confidence: 99%

Effect of neurotrophin‐3 on reinnervation of the larynx using the phrenic nerve transfer technique

Kingham

Hughes

Mitchard

et al. 2007

Eur J of Neuroscience

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Current techniques for reinnervation of the larynx following recurrent laryngeal nerve (RLN) injury are limited by synkinesis, which prevents functional recovery. Treatment with neurotrophins (NT) may enhance nerve regeneration and encourage more accurate reinnervation. This study presents the results of using the phrenic nerve transfer method, combined with NT-3 treatment, to selectively reinnervate the posterior cricoarytenoid (PCA) abductor muscle in a pig nerve injury model. RLN transection altered the phenotype and morphology of laryngeal muscles. In both the PCA and thyroarytenoid (TA) adductor muscle, fast type myosin heavy chain (MyHC) protein was decreased while slow type MyHC was increased. These changes were accompanied with a significant reduction in muscle fibre diameter. Following nerve repair there was a progressive normalization of MyHC phenotype and increased muscle fibre diameter in the PCA but not the TA muscle. This correlated with enhanced abductor function indicating the phrenic nerve accurately reinnervated the PCA muscle. Treatment with NT-3 significantly enhanced phrenic nerve regeneration but led to only a small increase in the number of reinnervated PCA muscle fibres and minimal effect on abductor muscle phenotype and morphology. Therefore, work exploring other growth factors, either alone or in combination with NT-3, is required.

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Section: Discussionmentioning

confidence: 99%

Effect of neurotrophin‐3 on reinnervation of the larynx using the phrenic nerve transfer technique

Kingham

Hughes

Mitchard

et al. 2007

Eur J of Neuroscience

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“…To quantify the glottal area, the glottal images with full abduction and adduction captured by PhotoImpact were dragged into the worksheet of the PhotoShop (Adobe, San Jose, CA). After the free edges of the vocal folds in each image were traced (24), the number of pixels encompassed by the vocal fold edges was then displayed in the histogram dialog box. Changes in the glottal area during respiratory cycle and in response to intravenous administration of capsaicin could be calculated by comparing the number of pixels encompassed by a reference area taken simultaneously with the images.…”

Section: Methodsmentioning

confidence: 99%

Capsaicin-induced activation of pulmonary vagal C fibers produces reflex laryngeal closure in the rat

Lee

Hwang

2006

Journal of Applied Physiology

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Our recent studies show that intravenous administration of capsaicin induces enhancement of the intralaryngeal thyroarytenoid (TA) branch but a reduction of the intralaryngeal abducent branch, suggesting that the glottis is likely closed by capsaicin. The aim of the present study was to examine whether the glottis is adducted by intravenous administration of capsaicin. Electromyographic (EMG) activity of the TA muscle, subglottal pressure (SGP), and glottal behavior were evaluated before and after intravenous administration of capsaicin in male Wistar rats that were anesthetized and tracheostomized. Catheters were placed in the femoral artery and vein, as well as in the right jugular vein. Low and high doses of capsaicin (0.625 and 1.25 microg/kg) produced apnea and increases in the amplitude of the TA EMG. This enhancement of the TA EMG was observed during apnea as well as during recovery from apnea. Moreover, the onset of the TA EMG was advanced such that it commenced earlier during inspiration. Concomitantly, the SGP substantially increased. Increases in both the TA EMG and SGP were abolished after bilateral sectioning of the recurrent laryngeal nerve. In some animals, movement of the vocal folds was recorded by taking a motion picture with a digital camera under a surgical microscope. With intravenous administration of capsaicin, a tight glottal closure, decreases in blood pressure, and bradycardia were observed. These results strongly suggest that glottal closure is reflexively induced by intravenous administration of capsaicin and that closure of the glottis is beneficial for the defense of the airway and lungs when an animal is exposed to environmental irritants.

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“…Studies examining the use of growth factors on denervation-related TA atrophy have shown promising results and have highlighted the importance of studying these factors in relation to agerelated atrophy. [195][196][197][198] According to the World Health Organization, those over 60 years of age are making up an everincreasing segment of the world population. 199 Although this realization clearly touts the societal and medical advances of recent years, it also offers considerable challenges to those providing care for this population.…”

Section: Future Directionsmentioning

confidence: 99%

Aging Thyroarytenoid and Limb Skeletal Muscle: Lessons in Contrast

2008

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Restoring Vocal Fold Movement after Transection and Immediate Suturing of the Recurrent Laryngeal Nerve with Local Application of Basic Fibroblast Growth Factor: An Experimental Study in the Rat

Abstract: bFGF facilitates regeneration of the transected RLN and attenuation of intrinsic laryngeal muscle atrophy, thereby restoring laryngeal function.

Cited by 24 publications

References 29 publications

Effect of neurotrophin‐3 on reinnervation of the larynx using the phrenic nerve transfer technique

Effect of neurotrophin‐3 on reinnervation of the larynx using the phrenic nerve transfer technique

Capsaicin-induced activation of pulmonary vagal C fibers produces reflex laryngeal closure in the rat

Aging Thyroarytenoid and Limb Skeletal Muscle: Lessons in Contrast

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