Restriction enzyme-mediated insertional mutagenesis: an efficient method of Rosellinia necatrix transformation

Attri, Chandrika; Swati, Swati; Kulshrestha, Saurabh

doi:10.1007/s00203-017-1466-y

Cited by 5 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the crude extracts of the fungus showed significant inhibition against the test pathogens F. Oxysporum , R. necatrix , C. xanthochromaticum , and Sclerotinia sclerotiorum . Rosellinia necatrix is a cosmopolitan fungus that is found all over the world and has a high ability to kill infected trees Attri and Kulshrestha [ 24 ]. Fusarium can infect a variety of crops, including rice, ornamentals, wheat, and all horticultural crops [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Kumari

Attri

et al. 2022

BioMed Research International

Self Cite

View full text Add to dashboard Cite

To discover new natural resources with biological effects, the chemical investigation of antioxidant and antimicrobial activities of extract’s Chaetomium globosum isolated from roots of Urginea indica. Gas chromatography-mass spectrometry (GC-MS) analysis demonstrated the presence of the major chemical constituents present in the methanol extract (1,3-oxathiolane, 1,3-cyclopentadiene, 5-(1-methylethylidene), 5,9-hexadecadienoic acid, methyl ester, decane), chloroform extract (acetic acid, diethoxy-, ethyl ester, 2,2-bis(ethylsulfonyl)propane, 3-methyl-2-(2-oxopropyl) furan), and hexane extract (3-hexanone, 4,4-dimethyl, decane,2,6-dimethyldecane, decane, 2,4,6-trimethyl, decane, 2,4,6-trimethyl, 1-butanesulfinamide, 1,1,2,2,3,3,4,4,4-nonafluoro-N-methyl, decane). The total compound identified (56.2%) in chloroform extract, (54.72%) in hexane extract, and (65%) in methanol extract. The antioxidant effects were performed using diphenylpicrylhydrazyl radical (DPPH). The results showed that the methanol extract showed significantly the highest anti-DPPH with an IC50 value of 37.61 ± 1.37 μg/mL, followed by chloroform and hexane extracts with IC50 values of 40.82 ± 3.60 and 45.20 ± 2.54 μg/mL, respectively. The antifungal activity of extracts was evaluated against pathogens fungi including Fusarium oxysporum, Rosellinia necatrix, Cladosporium xanthochromaticum, and Sclerotinia sclerotiorum. Methanolic and chloroform extracts showed maximum inhibition against all test pathogens, while hexane extract showed minimum inhibition.

show abstract

Section: Resultsmentioning

confidence: 99%

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Kumari

Attri

et al. 2022

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…Least changes were obtained with vacuum grinding, particularly in terms of preventing enzymatic browning and oxidation of antioxidants apples 43 .Rosellinia necatrix, causing root rot disease is a very destructive pathogen of woody plants and is responsible for yield losses to a large number of fruit trees. Out of 47 single spored transformants analyzed, only 33 showed hygromycin gene amplification using PCR and only 19 transformants showed single gene integration in southern hybridization, which accounted for single gene integration percentage of 42%, highest amongst all the previous reports on Rosellinia necatrix transformations 44 .…”

Section: Introductionmentioning

confidence: 82%

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

Kumar¹,

Saini²,

Meenakshi³

et al. 2018

J. Pharm. Sci. Innov.

View full text Add to dashboard Cite

In the past, the beneficial effects of fermented fruits were unknown, and so people primarily used fermentation to preserve fruits, enhance shelf life, and improve flavor. Fermentation of fruits has been adopted over many generations, primarily due to their commercial significance. Edible tropical fruits such as, cashew apple, mangoes, papaya, pineapple, litchi, guava, bael, banana, pomegranate, jamun and palm etc. with high export potential have their origin in the tropics and require rather suitable climatic conditions; and can not tolerate environmental stress. Most of the tropical fruits are important sources of antioxidants, vitamins and minerals and form a very healthy part of a diet. An effective utilisation of inferior grade and over-ripe fruits and processing them into fermented beverages has been revealed as a new and promising alternative to generate extra revenues whilst conducting a sustainable exploitation of wastes. In addition, microorganisms contributing to the fermentation process have recently been associated with many health benefits, and so these microorganisms have become another focus of attention. This paper reviews the production techniques and beneficial health effects of different fermented fruits. Scientific advances in the emerging area of functional beverages; as they provide a health benefit beyond the basic nutritional functions also reported in this paper.

show abstract

“…Fortunately, high-efficiency CaCl 2 -polyethylene glycol (PEG), A. tumefaciens, and restriction enzyme-mediated integration (REMI)-based transformation systems are available for R. necatrix (Kanematsu et al, 2004;Kano et al, 2011;Attri et al, 2018). Similarly, biolistic, PEG, lipofectin, and electroporation mediated transformation protocols have been reported for P. cinnamomi (Bailey et al, 1993;McCarren, 2006;Horta et al, 2008Horta et al, , 2010Chahed, 2016).…”

Section: Pathogen Transformation Toolsmentioning

confidence: 99%

Advances in Understanding Defense Mechanisms in Persea americana Against Phytophthora cinnamomi

et al. 2021

View full text Add to dashboard Cite

Avocado (Persea americana) is an economically important fruit crop world-wide, the production of which is challenged by notable root pathogens such as Phytophthora cinnamomi and Rosellinia necatrix. Arguably the most prevalent, P. cinnamomi, is a hemibiotrophic oomycete which causes Phytophthora root rot, leading to reduced yields and eventual tree death. Despite its’ importance, the development of molecular tools and resources have been historically limited, prohibiting significant progress toward understanding this important host-pathogen interaction. The development of a nested qPCR assay capable of quantifying P. cinnamomi during avocado infection has enabled us to distinguish avocado rootstocks as either resistant or tolerant - an important distinction when unraveling the defense response. This review will provide an overview of our current knowledge on the molecular defense pathways utilized in resistant avocado rootstock against P. cinnamomi. Notably, avocado demonstrates a biphasic phytohormone profile in response to P. cinnamomi infection which allows for the timely expression of pathogenesis-related genes via the NPR1 defense response pathway. Cell wall modification via callose deposition and lignification have also been implicated in the resistant response. Recent advances such as composite plant transformation, single nucleotide polymorphism (SNP) analyses as well as genomics and transcriptomics will complement existing molecular, histological, and biochemical assay studies and further elucidate avocado defense mechanisms.

show abstract

Restriction enzyme-mediated insertional mutagenesis: an efficient method of Rosellinia necatrix transformation

Cited by 5 publications

References 13 publications

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

GC‐MS Analysis, Antioxidant and Antifungal Studies of Different Extracts of Chaetomium globosum Isolated from Urginea indica

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

Advances in Understanding Defense Mechanisms in Persea americana Against Phytophthora cinnamomi

Contact Info

Product

Resources

About