Restriction Fragment Length Polymorphism for the Identification of Campylobacter jejuni-Isolates

Molecular subtyping by genome and plasmid analysis ofCampylobacter jejuniserogroups O1 and O2 (Penner) from sporadic and outbreak cases of human diarrhoea

“…Ribopatterns provide a well validated and reproducible means of discriminating between strains in many bacterial species [39], and have proved to be particularly effective in identifying strains of C. jejuni [14,17,[21][22][23][24]. We have shown in previous studies that overall ribopattern similarities broadly reflect the taxonomic affinities of strains within the thermophilic campylobacters.…”

“…Phage typing has been developed but utilized in only a limited number of studies on C. jejuni [13][14][15]. Similarly, DNA (molecular) typing, based on total genomic DNA digest patterns from conventional [14,[16][17][18][19] and pulse field gel electrophoresis [20], ribosomal RNA gene restriction digest patterns [14,17,[21][22][23][24], and plasmid profiling [25], has been applied to a limited extent. Recently, the polymerase chain reaction (PCR) was used to obtain randomly amplified polymorphic DNA (RAPD) profiles.…”

Serotyping of Campylobacter jejuni based on heat stable antigens: relevance, molecular basis and implications in pathogenesis

“…1991 ; Suzuki et al . 1994 ), ribosomal RNA gene restriction digest patterns ( Geilhausen et al . 1990 ; Owen et al .…”

Biotypes and DNA ribopatterns of thermophilic campylobacters from faeces and seawater in Eastern Spain

“…Epidemiological and diagonistic studies of C. jejuni are usually based on biotyping and serotyping, although the general utility of serology is limited by availability of suitable antisera (Penner 1988). Phage typing, plasmid analysis and protein electrophoresis (Gritliths & Park 1990) and more recently DNA-based techniques, in particul.ar restriction enzyme digest analysis and ribosomal RNA gene restriction (rDNA) patterns, have also been used to identify strains of Campylobacter (Owen et al 1990;Geilhausen et al 1990;Patron et al 1991). In this study, we provide new data on the biotypes and rRNA gene restriction patterns (ribopatterns) of campylobacters isolated from faeces of young children and from aquatic t Corresponding author (marine) sources in eastern Spain.…”