Restriction fragment length polymorphisms associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele

Cowan, C. M.; Dentine, M.R.; Ax, R. L.; Schuler, Linda A.

doi:10.1111/j.1365-2052.1989.tb00853.x

Cited by 30 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(1987) found RFLPs in the GH gene with the enzymes EcoRI and HindIII. However, our results are in agreement with the findings of Cowan et al (1989) and Hilbert et a/. [ 1989), since these enzymes detect no polymorphisms in this study.…”

Section: Hoj E+edh O Hsupporting

confidence: 93%

See 1 more Smart Citation

Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle

et al. 1993

View full text Add to dashboard Cite

Summary Fifty‐eight calves of both sexes from lines of Red Danish dairy breed selected for high (n= 36) and low (n= 22) milk fat production, and 32 heifers from lines of Norwegian Red dairy breed selected for high (n= 16) and low (n= 16) milk fat yield were typed for two previously reported restriction fragment length polymorphisms (RFLPs) in the growth hormone gene. The RFLPs are consistent with: (1) an insertion(I)/deletion(D) of approximately 0.9kb in the 3′‐region of the growth hormone gene and (2) a polymorphic MspI(+/−) site in the third intron. A traditional RFLP procedure was used for typing the I/D polymorphism and a polymerase chain reaction (PCR) procedure was developed for typing the Mspl polymorphism. Only the I‐MspI(+) and D‐MspI(‐) haplotypes were found. In the Red Danish lines the frequency of D‐MspI(‐) haplo‐type was 0.28 in high line and 0.05 in low line calves, this difference was significant (P <0.01). The corresponding frequencies in the Norwegian Red lines were 0.09 in the high line and 0.0 in the low line. Attempts to screen for RFLPs in the growth hormone receptor gene and in the insulin‐like growth factor‐I gene were unsuccessful.

show abstract

Section: Hoj E+edh O Hsupporting

confidence: 93%

“…These RFLPs have been shown to be present in several breeds (Beckmann et ul. 1986;Hallerman et al 1987;Cowan et al 1989;Hilbert et 01.1989;Theilmann et al 1989). In the following the two RFLPs are designated I/D site and MspI site.…”

Section: Introductionmentioning

confidence: 99%

Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle

et al. 1993

View full text Add to dashboard Cite

show abstract

“…Within the bovine PRL gene, several polymorphisms have been reported (Cowan et al, 1989;Hart et al, 1993;Zhang et al, 1993b;Chung and Kim, 1997 6). The PRL A allele was accepted as the predominant allele in cattle breeds, which is believed to have originated from Bos taurus (Sodhi et al, 2011).…”

Section: Prl Polymorphismmentioning

confidence: 99%

“…Within the bovine PRL gene, several polymorphisms have been reported (Cowan et al, 1989;Hart et al, 1993;Zhang et al, 1993b;Chung and Kim, 1997). The genotype and allele frequencies of the PRL gene for the studied cattle breeds are presented in Table 6).…”

Section: Prl Polymorphismmentioning

confidence: 99%

Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Ünal¹,

Kepenek²,

Dinç³

et al. 2015

Turk J Zool

View full text Add to dashboard Cite

IntroductionGenetic research in farm animals focuses mainly on the identification of genes influencing economically important traits that could be useful in breeding programs. In dairy cattle, most studies have researched the genes that may determine variations in milk yield and milk composition (Komisarek and Dorynek, 2009). The polymorphisms of several genes including growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) have been shown to affect milk yield and milk composition traits in dairy cattle.GH belongs to a family of somatolactogenic hormones including prolactin, placental lactogen, and a number of hematopoietic growth factors (Cosman et al., 1990). GH is located on the 19th chromosome in the q26-qter band region (Hediger et al., 1990). The GH gene of cattle is approximately 1800 bp in size and contains 5 exons and 4 introns. This gene encodes an mRNA with a size of 786 bp (Woychik et al., 1982). Although a number of polymorphisms have been observed in the GH gene of cattle, the 2 polymorphisms that are located in intron 3 and exon 5 have been found to be significant in their effects on milk and meat yield parameters (Lucy et al., 1991(Lucy et al., , 1993Høj et al., 1993). The polymorphism in intron 3 of GH is observed when GH is digested by the restriction enzyme MspI (GH-MspI) (Zhang et al., 1993). Two alleles are observed as a result of digestion with this enzyme. The first allele, GH-MspI ( -) contains a T insertion at the +837 position, and the second allele has a C-G transition at the +837 position (Lee et al., 1994). Zhang et al. (1993b) reported that the polymorphism in exon 5 could be observed when digested by the AluI enzyme (GH-AluI) and the 2 alleles called L (leucine in the 127th codon) and V (valine in the 127th codon) occur.The PRL gene is located on chromosome 23 and is composed of 5 exons and 4 introns (Camper et al., 1984, as cited by Skinkyte et al., 2005. A silent A-G mutation of the 103rd codon in exon 3 of the bovine PRL gene led to a polymorphic RsaI site (Lewin, 1992, as cited by Skinkyte et al., 2005). The polymorphism leading to the RsaI enzyme restriction site of the bovine PRL gene determines milk production traits including milk yield, milk fat, and milk protein contents (Lewin et al., 1992). The significant effect of the PRL locus on milk production traits was later shown by Chung et al. (1996, as cited by Dybus, 2002a as well.

show abstract

“…Leucine or H valine amino acid substitutions at residue 127 in GH exist due to allelic polymorphism (Sartore and Di Stasio 2000). A polymorphic site for Msp 1 restriction endonuclease was detected by Cowan et al (1989) and Hilbert et al (1989) and the polymorphism being localized in intron 3 of the GH gene in position 1,547 (Zhang H et al 1993). Studies on the effect of the GH-Msp1 polymorphism on production traits in Sahiwal cattle are quite advanced, but the results obtained by various workers are not always in agreement.…”

mentioning

confidence: 99%

Growth hormone-Msp1 loci polymorphism and its association with first lactation traits in Sahiwal cattle

Sachan

Gupta

Vineeth

et al. 2020

Indian J of Anim Sci

View full text Add to dashboard Cite

GH has important role in milk production and their polymorphisms may alter quality and quantity of gene's products and affects the phenotype. This study was designed to find the polymorphisms on GH-Msp1 loci and to find its association with milk production traits in Sahiwal cattle. Msp1 polymorphism of the bGH gene was identified in a population of Sahiwal cows. PCR-RFLP genotyping of 329 bp fragment of GH in exon 3 revealed three different allelic variants. The T allele was the major allele in Sahiwal cattle. The TT genotype Sahiwal cows yielded more FL305DMY, FLTMY and also had higher FLL than that of CT and CC genotypes cows. Results of the present findings reveal that TT allele of GH locus should be the favoured genotype in Sahiwal cattle breed and can be used in marker-assisted selection programmes.

show abstract

Restriction fragment length polymorphisms associated with growth hormone and prolactin genes in Holstein bulls: evidence for a novel growth hormone allele

Cited by 30 publications

References 19 publications

Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle

Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle

Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

Growth hormone-Msp1 loci polymorphism and its association with first lactation traits in Sahiwal cattle

Contact Info

Product

Resources

About