Restriction fragment length polymorphisms in diploid and allotetraploid Gossypium: Assigning the late embryogenesis-abundant (Lea) alloalleles in G. hirsutum

Ga, Galau; Bass, Hank W.; Dw, Hughes

doi:10.1007/bf00330608

Cited by 30 publications

(9 citation statements)

References 26 publications

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“…DNA was isolated from G. hirsuture (cv. S iokra Blue Tag) by a modification of the method of Galau [9]. DNA was cut with the enzymes indicated (50 #g each lane) transferred to nitrocellulose and probed with (A) the complete ( 1508 bp) Adh2a cDNA (pGhS31) and (B) the 3~-untranslated region of Adh2a (The 324 bp Ncol/EcoRI fragment).…”

Section: Four Classes Of Adh Genes Are Expressed Under Anaerobic Condmentioning

confidence: 99%

The alcohol dehydrogenase genes of cotton

Millar

Dennis

1996

Plant Mol Biol

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We report here the initial characterization of the alcohol dehydrogenase (Adh) gene family of the allotetraploid Gossypium hirsutum, a crop plant that is highly sensitive to waterlogging. Twelve Adh cDNAs were isolated from a library constructed from RNA prepared from anaerobically stressed root tips. Nine of the twelve cDNAs fell into one class, while each of the other three cDNAs fell into a separate class. The 3'-untranslated regions had little or no homology between classes implying that each of these four classes is encoded by a different gene. The most abundant class of Adh cDNA was expressed under the control of the 35S promoter in transgenic cotton and encodes the ADH2 isozyme, the isozyme induced most strongly by low oxygen conditions. Cotton Adh genomic segments were sequenced. The promoter regions of these Adh genes contain the cis-acting motifs which have been shown in other plant species to be necessary for anaerobic induction of transcription. A gene conversion event is likely to have occurred between the 3' ends of two of the genes. Sequence comparison of the Adh genomic and cDNA clones, and Southern analysis of genomic DNA suggest the existence of multiple copies of Adh genes in cotton. Five different members of this Adh gene family of cotton have been identified, of which four are very similar.

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Section: Four Classes Of Adh Genes Are Expressed Under Anaerobic Condmentioning

confidence: 99%

The alcohol dehydrogenase genes of cotton

Millar

Dennis

1996

Plant Mol Biol

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“…These are a random selection of mRNAs that comprise at least 0.2% of cotyledon mRNA between 28 days postanthesis (DPA) and 2 days postimbibition (DPI). Each of the 47 clones recognize different mRNAs at the standard hybridization criterion of Tm-15~ They do not crosshybridize with each other, and in most instances there are significant differences in either the size or expression patterns of the mRNAs to which they hybridize ( (Galau et al 1988). This also is true of all 26 new mRNA probes (data not shown).…”

Section: Isolation Of 26 Additional Mrna Sequencesmentioning

confidence: 99%

“…This also is true of all 26 new mRNA probes (data not shown). The exception is the VicA8 probe which hybridizes to about three genes in diploids and twice that number in the allotetraploids (Chlan et al 1987;Galau et al 1988). …”

Section: Isolation Of 26 Additional Mrna Sequencesmentioning

confidence: 99%

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Temporally modular gene expression during cotyledon development.

Hughes

Galau²

1989

Genes Dev.

176

149

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The regulation of cotton embryogenesis has been addressed by measuring the abundance of 47 mRNAs in cotyledons from the late cotyledon stage through early germination. There are at least 11 distinct classes of coordinately expressed mRNAs. Their expression patterns appear to result from unique combinations of five temporal abundance components. These are associated with the cotyledon stage, the endogenous concentration of free abscisic acid, maturation (reserve accumulation}, ovule abscission, and germination. This modularity suggests that only a few global regulatory factors orchestrate gene expression with many genes responding to several of them. Significant expression associated only with postabscission or free abscisic acid is restricted to that of the Lea mRNAs earlier suggested to be a component of the embryo's preparation for desiccation.

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“…Although phylogenetic trees have previously been established for many species on the basis of isozyme markers and chromosome homology (Rick, 1979;Riley, 1965;Beasley, 1942;Kimber, 1961;Philips, 1962), DNA markers have recently added to the breadth of phylogenetic information available (Song et al, 1988;Galau et al, 1988;Debener et al, 1990;Miller and Tanksley, 1991). Such studies are important in classifying newly discovered germplasm, and in establishing possible sources from which valuable traits might readily be transferred to crop species.…”

Section: Evolutionary Relationships and Comparative Mappingmentioning

confidence: 99%

Cassava Genetic Improvement

Ceballos¹,

Fregene²,

Carlos³

et al. 2007

Breeding Major Food Staples

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PREFACEThis Handbook is the result of a combined effort by several current and previous cassava researchers at CIAT to review and summarize the most important results of cassava research during the past 40 years. Most of the chapters are based on the various presentations during the Regional Cassava Training Course, held in Thailand from October 6 to 17, 2008. This course was organized upon the realization that many people that were actively involved with cassava research in the 1970s and 80s, both at CIAT and in national programs, have now retired or will soon do so, and that a whole new generation of cassava researchers are currently being hired to take their place.As cassava is now becoming a very important, and mostly industrial, crop in Asia, there are many new opportunities, but also a host of new problems and challenges. These include the appearance in Asia of new cassava diseases and pests; the decreasing availability and increasing cost of rural labor, resulting in the need for partial or complete mechanization of cassava production; the rapidly increasing demand for cassava roots for production of food, feed and fuel, and the unavailability in many countries of new land for any expansion of cassava area, thus requiring a rapid increase in cassava yields to increase supplies. This requires a renewed focus on cassava research for the development of new higher-yielding varieties and more sustainable production practices.While many cassava researchers in national programs in Asia received individual or group training at CIAT-Colombia during the 1970s and 80s, this training was greatly reduced during the following two decades due to funding limitations. Thus, the objective of the Regional Cassava Training Course in 2008 was to provide a new training opportunity for young scientists in Asian countries, and to hand over the knowledge and experience of the older cassava researchers, mostly from CIAT, to a new generation that will have to face the new challenges. Thus, the course was taught mostly by current or already retired CIAT cassava researchers, while the 60-plus participants of the course included cassava researchers from Cambodia, China, East Timor, India, Indonesia, Lao PDR, Malaysia, Myanmar, the Philippines, Thailand and Vietnam. The training course not only provided knowledge -from physiology and biotechnology to animal feeding and production of fuelethanol -but also an opportunity for people from different institutions and countries to get to know each other, which will greatly facilitate future collaboration.Course participants returned home with a CD containing the PowerPoint presentations of the course. However, it was felt that a more comprehensive review of all the topics covered was warranted, as this would provide more in-depth knowledge for those working in the various specialized fields. Most of this information is available in many refereed journals, in workshop proceedings and old CIAT annual reports, but many of these are now out of print or otherwise difficult to obtain. Thus, the Cas...

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Restriction fragment length polymorphisms in diploid and allotetraploid Gossypium: Assigning the late embryogenesis-abundant (Lea) alloalleles in G. hirsutum

Cited by 30 publications

References 26 publications

The alcohol dehydrogenase genes of cotton

The alcohol dehydrogenase genes of cotton

Temporally modular gene expression during cotyledon development.

Cassava Genetic Improvement

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