Restriction Inhibition Assay: A Qualitative and Quantitative Method to Screen Sequence Specific DNA Binder from Herbal Plants

Hassan, Syed Asif; Barthwal, Ritu; Padmadeo, Shree R; Barukab, Omar

doi:10.4314/tjpr.v13i2.16

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…Arborine was found to be the most active inhibitor, with relatively strong activity against EcoRI, PstI, and SalI, whereas moderate activity against BamHI, a Type 2A enzyme. The strong sequence-specific binding affinity of arborine in the 5'-GAATTC-3' restriction site of EcoRI demonstrates its potential therapeutic property as the sequence has also been observed in BRCA 2 breast cancer 2, an early onset oncogene (Hassan et al, 2014). The positive control, ciprofloxacin, exhibited inhibition activity against DraI both in the dark and under UVA irradiation.…”

Section: Photo-activated Enzymatic Restriction Inhibitory Activitymentioning

confidence: 90%

Bioautography, Combination Effects and Photo-Activated Enzymatic Restriction Inhibitory Activity of Antimicrobial Alkaloids From Glycosmis

Taib

Hassan²,

Kamal³

et al. 2022

MJS

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Glycosmis pentaphylla (Retz.) DC., locally known as nerapan, has long been used in Asian countries as a traditional remedy for ailments attributed to microbial infections. This study aims to isolate antimicrobial alkaloids from G. pentaphylla, to determine their combination effects with selected antimicrobial agents and to screen for their photoactivated enzymatic restriction inhibitory activity. Bioautography-guided isolation of antimicrobial alkaloids was performed by using column chromatography with Staphylococcus aureus, Escherichia coli, and Candida albicans as the indicator microbes. The antimicrobial effects of the alkaloids combined with selected antimicrobial agents, namely, ciprofloxacin, erythromycin, vancomycin, and ketoconazole, were determined by using a checkerboard assay. Photoactivated enzymatic restriction inhibitory activity was assessed by using agarose gel electrophoresis. Two antimicrobial active alkaloids were isolated and identified as arborinine and arborine. The antimicrobial activity of arborinine and arborine was determined to be in the range of 250 µg/ml and 1000 µg/ml. Partial synergy was observed for all arborine-antibiotics and arborinine-ketoconazole interactions against S. aureus and C. albicans, respectively. Arborine was relatively the strongest photoactivated enzymatic restriction inhibitor, particularly against EcoRI, PstI, and SalI. The results obtained are promising and encourage further research on alkaloids as potential antimicrobial-enhancing agents.

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Section: Photo-activated Enzymatic Restriction Inhibitory Activitymentioning

confidence: 90%

Bioautography, Combination Effects and Photo-Activated Enzymatic Restriction Inhibitory Activity of Antimicrobial Alkaloids From Glycosmis

Taib

Hassan²,

Kamal³

et al. 2022

MJS

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show abstract

Phosphorus-nitrogen compounds: Part 46. The reactions of N3P3Cl6 with bidentate and monodentate ligands: The syntheses, structural characterizations, antimicrobial and cytotoxic activities, and DNA interactions of (N/N)spirocyclotriphosphazenes with 4-chlorobenzyl pendant arm

Asmafiliz

Berberoğlu

Özgür

et al. 2019

Inorganica Chimica Acta

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Molecular structure, spectroscopic (IR, Raman, NMR, UV–vis) and molecular docking studies of an anticancer drug: isoDC81

Tiwari

Kumar

Sharma

2021

Materials Today: Proceedings

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Restriction Inhibition Assay: A Qualitative and Quantitative Method to Screen Sequence Specific DNA Binder from Herbal Plants

Abstract: Purpose: To employ restriction inhibition assay (RIA) to screen phytochemical-rich fractions (PRFs) with high affinity for EcoRI and in the ESTs of BRAC2 provides an insight into the use of screened components as leads in the search for novel anticancer compounds.

Cited by 4 publications

References 20 publications

Bioautography, Combination Effects and Photo-Activated Enzymatic Restriction Inhibitory Activity of Antimicrobial Alkaloids From Glycosmis

Bioautography, Combination Effects and Photo-Activated Enzymatic Restriction Inhibitory Activity of Antimicrobial Alkaloids From Glycosmis

Phosphorus-nitrogen compounds: Part 46. The reactions of N3P3Cl6 with bidentate and monodentate ligands: The syntheses, structural characterizations, antimicrobial and cytotoxic activities, and DNA interactions of (N/N)spirocyclotriphosphazenes with 4-chlorobenzyl pendant arm

Molecular structure, spectroscopic (IR, Raman, NMR, UV–vis) and molecular docking studies of an anticancer drug: isoDC81

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