Results of German multicenter study of antimicrobial susceptibilities of Trichophyton rubrum and Trichophyton mentagrophytes strains causing tinea unguium. German Collaborative Dermatophyte Drug Susceptibility Study Group

Körting, Hans Christian; Ollert, Markus; Abeck, Dietrich

doi:10.1128/aac.39.5.1206

Cited by 73 publications

(71 citation statements)

References 18 publications

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“…The evaluation of in vitro activities of tested drugs revealed that terbinafine was the most potent active drug, confirming reports by Korting et al (13) and Fernández-Torres et al (6). Between the azoles, itraconazole was the most active, followed by ketoconazole and fluconazole.…”

Section: Discussionsupporting

confidence: 75%

Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

Santos

Hamdan

2005

J Clin Microbiol

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Fifty clinical isolates ofwere included as quality controls. All isolates produced clearly detectable growth only after 7 days of incubation. MICs were significantly independent of the incubation temperature (28 or 35°C) (P < 0.05). Different incubation periods resulted in MICs which were consistently different for each medium when azoles and griseofulvin were tested (P < 0.05). MICs obtained from different media at the same incubation time for the same isolate were significantly different when azoles and griseofulvin were tested (P < 0.05). MICs were consistently higher (usually 1 to 2 dilutions) with RPMI than with MVM or SDB (P < 0.05). When terbinafine was tested, no parameter had any influence on MICs (P < 0.05). RPMI standard medium appears to be a suitable testing medium for determining the MICs for T. rubrum. MICs obtained at different incubation times need to be correlated with clinical outcome to demonstrate which time has better reliability.

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Section: Discussionsupporting

confidence: 75%

Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

Santos

Hamdan

2005

J Clin Microbiol

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“…Similar results were obtained in a multicenter study performed by Espinel-Ingroff et al (8), who, testing filamentous fungi, found the lowest intra-and interlaboratory agreement for ITC (59 to 79% and 59 to 91%, respectively). Although several studies have been published on the in vitro susceptibility of the three drugs tested here (15,17,18,24,27), it is difficult to compare results due to variability in the different methods and conditions under which they were performed. We observed in general that TRB was more active than CLT and ITC.…”

Section: Discussionmentioning

confidence: 99%

Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes

et al. 2002

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A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 10 3 and 10 4 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra-and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 10 4 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.Dermatophytes are a group of morphologically and physiologically related molds that cause well-defined infections in vertebrates. The incidence of dermatophytoses has increased over recent years, particularly in immunocompromised patients (29,30,32,33). The choice of the proper treatment is determined by the site and extent of the infection and the species involved, as well as by the efficacy, safety profile, and kinetics of the available drugs. For localized nonextensive lesions, topical therapies with clotrimazole (CLT) are generally used. For tinea unguium, scalp ringworm, extensive infections, or skin lesions with folliculitis, systemic antifungal treatment is necessary (1,4,23,26). Oral drugs such as itraconazole (ITC) and terbinafine (TRB) are the antifungal agents currently most used to treat severe infections (4, 23). Some novel compounds, such as UR-9825, posaconazole, voriconazole, or ravuconazole, also appear to be promising candidates for the treatment of dermatophytosis (2,10,28).In the in vitro method proposed by the National Committee for Clinical Laboratory Standards (NCCLS) for testing molds (25), the dermatophytes were not included. Therefore, it is necessary to develop a reproducible standardized method for these important fungi that would lead to protocols for proper treatment. In recent years, some authors, possibly encouraged by the development of the above-mentioned reference method, have published various articles wherein several species of dermatophytes have been tested (11,27,28,34). In these works, different adaptations or modifications of the NCCLS methods have been assayed, although other techniques have also been used (3,12,15). The results obtained have been clearly contradictory in some aspects, which makes evident the need for standardization and the development of reference methods. Recently, we evaluated the activity of 11 antifungal drugs against an important number of strains of dermatophytes (n ϭ 508) by using a microdilution method (10). In that study the testing conditions adopted were an inoculum size of 10 4 CFU/ml, a temperature and time of incubation of 28°C and 7 days, respectively, and the MIC endpoint determination was 50% growth inhibition for azoles and 100% for the rest. It is unknown whether varying the conditions would have changed the results significantly....

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“…Similar results have been verified by other authors that showed that these drugs had low MICs against dermatophytes 10,12,15 . These low MICs found for the three drugs can help to explain the promising results obtained for the treatment of dermatophytosis with these antifungal agents 19 .…”

Section: Discussionmentioning

confidence: 99%

“…Although a reference method for dermatophytes, is not available, a good correlation between the in vitro data, using broth microdilution method, and clinical outcome has been demonstrated 19 .…”

Section: Discussionmentioning

confidence: 99%

In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method

Araujo¹,

Miranda²,

Fernandes³

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from Goiania city patients, Brazil. In this study, the microtiter plates were incubated at 28 o C allowing a reading of the minimal inhibitory concentration (MIC) after four days of incubation for Trichophyton mentagrophytes and five days for T. rubrum and Microsporum canis. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03 µg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole, ketoconazole and terbinafine, respectively.

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Results of German multicenter study of antimicrobial susceptibilities of Trichophyton rubrum and Trichophyton mentagrophytes strains causing tinea unguium. German Collaborative Dermatophyte Drug Susceptibility Study Group

Cited by 73 publications

References 18 publications

Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

Evaluation of Broth Microdilution Antifungal Susceptibility Testing Conditions for Trichophyton rubrum

Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes

In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method

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