Resveratrol accumulation and its involvement in stilbene synthetic pathway of Chinese wild grapes during berry development using quantitative proteome analysis

Li, Ruimin; Xie, Xiaoqing; Ma, Fengwang; Wang, Dan; Wang, Lan; Zhang, Jianxia; Xu, Yan; Zhang, Chaohong; Wang, Yuejin

doi:10.1038/s41598-017-10171-x

Cited by 13 publications

(7 citation statements)

References 67 publications

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“…Therefore, further growth of the resveratrol market is expected. Resveratrol currently available on the market is predominantly extracted from certain plants including grape, peanut, and the Japanese knotweed Polygonum cuspidatum ( Chen et al, 2013 ; Chukwumah et al, 2009 ; Li et al, 2017 ). However, due to the unstable harvest of these plants and the low resveratrol purity obtained, it is necessary to develop a stable production process of high-purity resveratrol from inexpensive feedstocks.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Kobayashi

Inokuma

Matsuda

et al. 2021

Metabolic Engineering Communications

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Resveratrol is a plant-derived aromatic compound with a wide range of beneficial properties including antioxidant and anti-aging effects. The resveratrol currently available on the market is predominantly extracted from certain plants such as grape and the Japanese knotweed Polygonum cuspidatum . Due to the unstable harvest of these plants and the low resveratrol purity obtained, it is necessary to develop a stable production process of high-purity resveratrol from inexpensive feedstocks. Here, we attempted to produce resveratrol from a wide range of sugars as carbon sources by a using the genetically-engineered yeast Scheffersomyces stipitis (formerly known as Pichia stipitis ), which possesses a broad sugar utilization capacity. First, we constructed the resveratrol producing strain by introducing genes coding the essential enzymes for resveratrol biosynthesis [tyrosine ammonia-lyase 1 derived from Herpetosiphon aurantiacus ( HaTAL1 ), 4-coumarate: CoA ligase 2 derived from Arabidopsis thaliana ( At4CL2 ), and stilbene synthase 1 derived from Vitis vinifera ( VvVST1 )]. Subsequently, a feedback-insensitive allele of chorismate mutase was overexpressed in the constructed strain to improve resveratrol production. The constructed strain successfully produced resveratrol from a broad range of biomass-derived sugars [glucose, fructose, xylose, N -acetyl glucosamine (GlcNAc), galactose, cellobiose, maltose, and sucrose] in shake flask cultivation. Significant resveratrol titers were detected in cellobiose and sucrose fermentation (529.8 and 668.6 mg/L after 120 h fermentation, respectively), twice above the amount obtained with glucose (237.6 mg/L). Metabolomic analysis revealed an altered profile of the metabolites involved in the glycolysis and shikimate pathways, and also of cofactors and metabolites of energy metabolisms, depending on the substrate used. The levels of resveratrol precursors such as L-tyrosine increased in cellobiose and sucrose-grown cells. The results indicate that S. stipitis is an attractive microbial platform for resveratrol production from broad types of biomass-derived sugars and the selection of suitable substrates is crucial for improving resveratrol productivity of this yeast.

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Section: Introductionmentioning

confidence: 99%

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Kobayashi

Inokuma

Matsuda

et al. 2021

Metabolic Engineering Communications

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“…To further study the properties of high stilbene content and high resistance to powdery mildew, multiomics analysis of Danfeng-2 and V. vinifera 'Cabernet Sauvignon' was carried out. Interestingly, analysis of Danfeng-2 transcriptome data (PRJNA306731) revealed many novel transcripts that could not be mapped on the grapevine reference genome (PN40024), including six novel STS transcripts (Li, 2019;Li et al, 2017). However, whether these newly discovered VqNSTS genes contribute to the high levels of resveratrol and the high resistance to U. necator in Danfeng-2 is still unclear.…”

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confidence: 99%

Alfin‐like transcription factor VqAL4 regulates a stilbene synthase to enhance powdery mildew resistance in grapevine

Yan

Yang

et al. 2022

Molecular Plant Pathology

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Resveratrol is a phytoalexin that is synthesized by stilbene synthase (STS). Resveratrol in the human diet is known to have beneficial effects on health. We previously identified six novel STS (VqNSTS) transcripts from the transcriptome data of Vitis quinquangularis accession Danfeng‐2. However, the functions of and defensive mechanisms triggered by these VqNSTS transcripts remain unknown. In the present study, we demonstrate that the expression of five of these six novel members, VqNSTS2–VqNSTS6, can be induced by the powdery mildew‐causing fungus Uncinula necator. Additionally, overexpression of VqNSTS4 in the V. vinifera susceptible cultivar Thompson Seedless promoted accumulation of stilbenes and enhanced resistance to U. necator by activating salicylic acid (SA) signalling. Furthermore, our results indicate that the Alfin‐like (AL) transcription factor VqAL4 can directly bind to the G‐rich element (CACCTC) in the VqNSTS4 promoter and activate gene expression. Moreover, overexpression of VqAL4 in Thompson Seedless enhanced resistance to U. necator by promoting stilbene accumulation and activating SA signalling. Conversely, RNA interference‐mediated silencing of VqNSTS4 and VqAL4 resulted in increased susceptibility to U. necator. Collectively, our results reveal that VqNSTS4, regulated by VqAL4, enhances grapevine resistance to powdery mildew by activating SA signalling. Our findings may be useful to improve disease resistance in perennial fruit trees.

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“…These compounds can contribute bitter and astringent characteristics to grapes and wines, impacting overall flavor and mouthfeel properties. Wild varieties are an incredibly valuable genetic resource for grape breeding, particularly for enhancing metabolites such as resveratrol [ 56 ], as well as for disease resistance [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

A Field Collection of Indigenous Grapevines as a Valuable Repository for Applied Research

Shecori

Kher²,

Tyagi

et al. 2022

Plants

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The grapevine is an economically important plant, with a historical connection to the development of human culture. Currently, over 6000 accessions are known as individual grapevine varieties, some of which are important to national heritage, valuable for current viticultural practices, and as genetic resources to maintain plasticity under changing climatic conditions, environmental sustainability, and market demands. Recently, the diversity of cultivated grapevines has declined significantly, due to the increased focus of global wine industries on a few major cultivars. Moreover, due to biotic and abiotic stresses, the wild V. vinifera germplasm’s genetic diversity has declined, with some varieties on the verge of extinction. Vitis germplasm conservation can be achieved via either in situ (e.g., protected areas) or ex situ (e.g., field collections, seed banks, and tissue culture collections) methods. This study aims to highlight the importance of Vitis field bank collections. We demonstrate the research done in the Israeli indigenous Vitis vinifera collection. The multi-layer analysis of the varieties enabled the identification of drought stress-resistant varieties, and suggested a mechanism for this resistance through noting the dramatic phenological differences in foliage development between resistant and sensitive varieties. In addition, we show a general characterization of the varieties via major grape characteristics, including bunch and berry shape, as well as their possible utilization based on their aromatic and phenolic profiles.

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Resveratrol accumulation and its involvement in stilbene synthetic pathway of Chinese wild grapes during berry development using quantitative proteome analysis

Cited by 13 publications

References 67 publications

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Alfin‐like transcription factor VqAL4 regulates a stilbene synthase to enhance powdery mildew resistance in grapevine

A Field Collection of Indigenous Grapevines as a Valuable Repository for Applied Research

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