Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines

Abstract

Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 μM of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate (32Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, ρACCβ, pAMPKα, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of ρACCβ (3.3-fold) and AMPKα/ρAMPKα (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of ρACCβ (111.8-fold) and AMPKα/ρAMPKα (1.2 fold). Our results show that Resv modified PL and saturated and unsaturated FA especially in MDA-MB-231 cells, and open new perspectives to the understanding of the reported anticancer effect of Resv on these cells.

Targeting AMPK Signaling by Dietary Polyphenols in Cancer Prevention

“…[ 145 ] Dietary polyphenols trigger the activation of AMPK in cancer cells, leading to disorders of lipid metabolism and carbohydrate metabolism, and weakening the antioxidant capacity of cancer cells. [ 146,147 ] AMPK is the important target of dietary polyphenols to inhibit cancer cell proliferation and promote apoptosis. Phosphoproteomic analysis was used to evaluate the effect of curcumin on colon cancer cells, results showed that AMPK was important molecular target for cancer intervention.…”

The Anti-Cancer Effects of Mitochondrial-Targeted Triphenylphosphonium–Resveratrol Conjugate on Breast Cancer Cells

“…It has been reported that resveratrol-mediated lipidomic alterations can significantly affect glycerophospholipid metabolism [ 34 ]. Luciana Gomes found that phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were decreased in MDA-MB-231 cells after resveratrol treatment [ 35 ]. Our study observed an increasing tendency in glycerophospholipids, including lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), as shown in Table 1 .…”

Bioactive Compounds and Metabolites from Grapes and Red Wine in Breast Cancer Chemoprevention and Therapy

“…Metabolism changes are often associated with resistance to chemotherapy and therapeutic sensitivity in clinical chemotherapy. Breast cancer cells not only show significant differences in metabolism compared with healthy breast cells, but also show differences in drug resistance [233,234]. Cancer and metabolism are deeply interconnected, studies indicate that cancer evolution is associated with abnormal glucose metabolism that is related to high proliferation, metastasis and clinical characteristics and is allied to the action of a particular drug.…”

“…Cancer and metabolism are deeply interconnected, studies indicate that cancer evolution is associated with abnormal glucose metabolism that is related to high proliferation, metastasis and clinical characteristics and is allied to the action of a particular drug. In this context, chemoresistance enables cancer cells to survive drug action and proliferate uncontrollably, which may lead to strong metastatic potential and cancer progression [230][231][232][233][234].…”