Resveratrol modulates the inflammatory response in hPDLSCs via the NRF2/HO‐1 and NF‐κB pathways and promotes osteogenic differentiation

Abstract

ObjectiveThe purpose of this study was to investigate resveratrol's specific role as an anti‐inflammatory and osteogenic differentiation of hPDLSCs in periodontitis and to reveal the mechanisms involved.BackgroundNumerous studies have shown that inhibiting the inflammatory response of periodontal tissues and promoting the regeneration of alveolar bone are crucial treatments for periodontitis. Resveratrol has been found to have certain anti‐inflammatory property. However, the anti‐inflammatory mechanism and osteogenic effect of resveratrol in periodontitis are poorly understood.Materials and MethodsWe constructed an in vitro periodontitis model by LPS stimulation of hPDLSCs and performed WB, RT‐qPCR, and immunofluorescence to analyze inflammatory factors and related pathways. In addition, we explored the osteogenic ability of resveratrol in in vitro models.ResultsIn vitro, resveratrol ameliorated the inflammatory response associated with activation of the NF‐κB pathway through activation of the NRF2/HO‐1 pathway, characterized by inhibition of p65/p50 nuclear translocation and the proinflammatory cytokines interleukin‐1β levels. Resveratrol also has a positive effect on osteogenic differentiation.ConclusionsObservations suggest that resveratrol modulates the inflammatory response in hPDLSCs via the NRF2/HO‐1 and NF‐κB pathways and promotes osteogenic differentiation.

The Genetic Aspects of Periodontitis Pathogenesis and the Regenerative Properties of Stem Cells

“…Additionally, PDLSCs have been investigated after the treatment with resveratrol, an anti-inflammatory phytoestrogen, which promoted the viability and osteogenic markers of PDLSCs [159]. Similar results were demonstrated in a recent study by Ma et al [160]. Importantly, natural agents affect not only PDLSCs gene expression but also their paracrine properties.…”

Traditional Chinese medicine treats ulcerative colitis by regulating gut microbiota, signaling pathway and cytokine: Future novel method option for pharmacotherapy

“…RSV is extracted from the rhizome of Reynoutria japonica Houtt., and has anti-inflammatory, antioxidant, and cardiovascular protective activities [ 115 ]. The main mechanisms of RSV for the treatment of UC are the inhibition of PI3K/AKT, MAPK, Notch1, and NF-κB signaling pathways and downregulation of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 levels [ 116 ].…”