Retention Indices for Frequently Reported Compounds of Plant Essential Oils

Babushok, Valeri I.; Linstrom, Peter J.; Зенкевич, И. Г.

doi:10.1063/1.3653552

Cited by 720 publications

(445 citation statements)

References 79 publications

(53 reference statements)

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“…Los índices de retención lineales (IRL) se calcularon con base en la serie homóloga de n-alcanos C9-C25 (Sigma-Aldrich, Milwaukee, Wisconsin, EE.UU.). Los componentes se identificaron comparando sus espectros de masas e IRL con espectros de bases de datos (NIST14 y Wiley) y reportados por otros (Davies, 1990;Babushok et al, 2011). Se emplearon además compuestos de referencia (Sigma-Aldrich).…”

Section: Análisis Químico De Aceites Esencialesunclassified

Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

et al. 2018

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RESUMENColombia posee gran diversidad de plantas medicinales, pero pocas han sido objeto de investigación. En este trabajo se evaluó la actividad antiproliferativa de aceites esenciales obtenidos por hidrodestilación asistida por microondas. Se analizaron 15 muestras de 11 especies en ensayos del MTT en células cancerosas MCF-7, HeLa y HepG-2 y se incluyeron células normales humanas (HEK293) y de animales (Vero y BF16F10) para evaluar selectividad. La composición química de muestras activas se determinó por cromatografía de gases acoplada a espectrometría de masas (GC-MS). Aceites esenciales de cuatro especies mostraron actividad antiproliferativa (CI 50 : 50 μg/mL) en células HeLa y/o MCF-7, en el siguiente rango (índice de selectividad en paréntesis): Piper cumanense H.B.K. (4,7) > Piper subflavum var. espejuelanum C.DC (3,9) > Salvia officinalis L. (3,6) > Piper eriopodom (Miq.) C. DC. (3,1). Ninguna muestra fue activa en células HepG-2. El análisis por CG-MS identificó β-cariofileno, α-copaeno, β-pineno, α-pineno y linalol como componentes mayoritarios. Los aceites esenciales activos pueden ser puntos de partida para desarrollo de medicamentos herbales para cuidado paliativo del cáncer.Palabras clave: aceite esencial, apoptosis, cáncer, fitoterapia, MTT. ABSTRACTColombia has a great diversity of medicinal plants, but few have been investigated. In this work, the antiproliferative activity of essential oils obtained by microwave-assisted hydrodistillation was evaluated. Fifteen samples from 11 species were analyzed in MTT assays in cancer cells MCF-7, HeLa and HepG-2, and normal human cells (HEK293) and animal cells (Vero and B16F10) were included to evaluate selectivity. The chemical composition of the active oils was determined by gas chromatography coupled to mass spectrometry (GC-MS). Essential oils from four species showed antiproliferative activity (IC 50 : <50 μg / mL) in HeLa and / or MCF-7 cells, in the following range (selectivity index in parenthesis): Piper cumanense H.B.K. (4.7) > Piper subflavum var. espejuelanum C. DC (3.9) > Salvia officinalis Linneo. (3.6) > Piper eriopodom (Miq.) C.DC. (31). The CG-MS analysis identified β-caryophyllene, α-copaene, β-pinene, α-pinene and linalool as major components. Active essential oils could serve as starting points to develop herbal medicines for cancer palliative care.

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Section: Análisis Químico De Aceites Esencialesunclassified

Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

et al. 2018

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“…Further identification was carried out by injecting a homologue series of n-alkanes (C 7-C 30 ) under identical chromatographic conditions, and calculating non-isothermal retention indices (RI). These RI were compared to those reported in the published literature (Adams 2007;Babushok et al 2011;Pherobase 2016). Relative proportions of the components were calculated based on GC-FID peak areas using response factors of 1 for each component.…”

Section: Analysis Of Essential Oils and Characterisation Of Plant Volmentioning

confidence: 99%

“…Mass spectra and RI reflected the data from the published literature (Runyoro et al 2010;Babushok et al 2011;Kpadonou Kpoviessi et al 2012). Six of these compounds were common to both Ocimum species (α-pinene, Camphene β-pinene, Myrcene, Limonene and Borneol).…”

Section: Chemical Components Of Essential Oils and Plant Volatilesmentioning

confidence: 99%

Oviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae)

Yarou

Bawin

Boullis

et al. 2017

Environ Sci Pollut Res

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The leafminer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most important pests of tomato, reducing crop yields by up to 100% in greenhouses and fields, in several countries globally. Because synthetic insecticides lead to resistance and have adverse effects on natural enemies and the health of producers, alternative control methods are needed. In this study, we assessed the oviposition-deterring effect of basil plants, Ocimum gratissimum L. and O. basilicum L. (Lamiaceae), using dual-choice behavioural assays performed in flight tunnels. We found that both plants significantly reduced T. absoluta oviposition behaviour on a tomato plant located nearby. To evaluate the potential effect of basil volatile organic compounds, we formulated essential oils of both plant species in paraffin oil, and observed a similar oviposition-deterring effect. Gas chromatography analyses detected 18 constituents in these essential oils which the major constituents included thymol (33.3%), p-cymene (20.4%), γ-terpinene (16.9%), myrcene (3.9%) in O. gratissimum and estragol (73.8%), linalool (8.6%), β-elemene (2.9%) and E-β-ocimene (2.6%) in O. basilicum. Twenty and 33 compounds were identified of the volatiles collected on O. gratissimum and O. basilicum plants, respectively. The main components include the following: p-cymene (33.5%), γ-terpinene (23.6%), α-terpinene (7.2%), α-thujene (6.7%) and E-α-bergamotene (38.9%) in O. gratissimum, and methyl eugenol (26.1%), E-β-ocimene (17.7%), and linalool (9.4%) in O. basilicum. Four compounds (α-pinene, β-pinene, Myrcene, Limonene) were common in essential oils and plants. Our results suggest the valuable potential of basil and associated essential oils as a component of integrated management strategies against the tomato leafminer.

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“…Compounds were identified by their retention indices determined using C8-C40 alkane standards and compared with literature data [11]. The identities were confirmed by the mass spectral data observed (Table 1) …”

Section: Identification Of the Compoundsmentioning

confidence: 88%

Essential Oil Chemotypes of Three Ocimum Species Found in Sierra Leone and Nigeria

Ta¹

2017

Med Aromat Plants

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Chemical composition of the essential oil of Ocimum basilicum L., O. gratissimum L. and O. americanum L. varieties found in Sierra Leone and Nigeria were determined by GC-FID and GC-MS. The antimicrobial test was evaluated by agar diffusion. The Sierra Leone and Nigeria O. americanum L. varieties were identified as the linalool chemotype and similarly, varieties of O. gratissimum L. from both countries are thymol chemotype. The high thymol content is consistent with the relative high antimicrobial activity of the O. gratissimum L oils. The Sierra Leone O. basilicum L. sample was established as the methyl eugenol chemotype while the Nigerian collection is predominantly methyl chavicol in composition. The high methyl eugenol content of the Sierra Leone collection is consistent with the observation of attraction of fruit flies to the distillate. To the best of our knowledge, there has been no report on essential oils of Ocimum species in Sierra Leone, hitherto.

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Retention Indices for Frequently Reported Compounds of Plant Essential Oils

Cited by 720 publications

References 79 publications

Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

Actividad antiproliferativa de aceites esenciales de plantas cultivadas en Colombia

Oviposition deterrent activity of basil plants and their essentials oils against Tuta absoluta (Lepidoptera: Gelechiidae)

Essential Oil Chemotypes of Three Ocimum Species Found in Sierra Leone and Nigeria

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