Retinal tissue preparation for high-resolution live imaging of photoreceptors expressing multiple transgenes

Haeri, Mohammad; Zhuo, Xinming; Haeri, Morteza; Knox, Barry E.

doi:10.1016/j.mex.2018.03.001

Cited by 1 publication

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“…The most common detection methods for miRNAs are qRT-PCR and microarray; they are strongly dependent on the precision of the extraction methods [75]. Therefore, it is imperative to optimize and standardize the miRNA isolation methods/ procedure [76]. Many studies reported discrepancies in miRNA measurement and recovery because of the extraction methods [68,77].…”

Section: Selection Of Sample Source and Mirnas Extraction Methodsmentioning

confidence: 99%

MicroRNAs: The next generation of cancer biomarkers

2023

Biomed. Lett.

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MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that have been shown to be involved in a wide range of biological processes, including cancer. miRNAs are known to regulate the expression of genes, and their dysregulation has been linked to the development of cancer. In recent years a great deal of attention is received by miRNAs due to their potential as biomarkers for cancer. Biomarkers are measurable indicators of a biological state, and they can be used to diagnose, monitor, and treat diseases. miRNAs can be detected in biological fluids such as blood and saliva. This makes them ideal candidates for early cancer detection and monitoring. We herein reviewed current methods for the isolation of circulating miRNAs. Provide the most recent update about clinical trials aiming at using miRNAs as biomarkers for cancer. Additionally, we highlighted some pitfalls that should be realized to take advantage of the massive potential of miRNAs as a cancer biomarker. However, the potential of miRNAs as cancer biomarkers is very promising but advancements in factors such as miRNA isolation methods, and the type of samples are critical to incorporate miRNA-based diagnostic and prognostic markers in modern-day treatment regimens for cancer. This review concludes that miRNAs have enormous clinical significance as cancer biomarkers and recommends carefully selecting methods for the isolation of miRNAs based on the type of sample, and the downstream applications to generate clinically relevant results.

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Section: Selection Of Sample Source and Mirnas Extraction Methodsmentioning

confidence: 99%

MicroRNAs: The next generation of cancer biomarkers

2023

Biomed. Lett.

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Retinal tissue preparation for high-resolution live imaging of photoreceptors expressing multiple transgenes

Abstract: Graphical abstract

Cited by 1 publication

References 20 publications

MicroRNAs: The next generation of cancer biomarkers

MicroRNAs: The next generation of cancer biomarkers

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