Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez, E.; Rodríguez, Aida; Dd́ez, Carmen; Royo, Luis J.; Moreira, Pedro; Caamaño, J. N.; Moran, E.; Gutiérrez‐Adán, Alfonso

doi:10.1002/mrd.20154

Cited by 30 publications

(28 citation statements)

References 54 publications

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“…This fact can be explained as oocytes submitted to a meiotic arrest could need a shorter period to accomplish nuclear maturation [37]. However, we decided to use a 24 h period of IVM in order to get comparable results to previous work [19][20][21][22].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, Faerge et al [17] described modifications in the nucleus of fresh bovine oocytes after meiotic inhibition with various chemical and biological inhibitors, and Lonergan et al [18] showed that inhibition of meiosis induced wide ultrastructural changes. Interestingly, these alterations coexist with embryonic development rates comparable to those of normal in vitro matured oocytes [19][20][21][22]. To date, no studies on ultrastructure of the cryopreserved cumulus-oocyte complexes (COCs) submitted to meiotic inhibition followed by IVM have been developed.…”

Section: Introductionmentioning

confidence: 99%

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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The nuclear stage at which oocytes are cryopreserved influences further development ability and cryopreservation affects ultrastructure of both cumulus cells and the oocyte. In this work, we analyze the effects of vitrification at different nuclear and cytoplasmic maturation stages on the oocyte ultrastructure and developmental ability. Culture in TCM199 + PVA with roscovitine 25 M during 24 h led to meiotic arrest (MA) in cumulus-oocyte complexes (COCs), while permissive in vitro maturation (IVM) was performed in TCM199, 10% FCS, FSH-LH and 17b-estradiol for 24 h. Oocytes were vitrified using the open pulled straw method (OPS) with minor modifications. Fresh and vitrified/warmed COCs were fixed as immature, after IVM, after meiotic arrest (MA) and after MA + IVM.Vitrification combined with MA followed by IVM produced the highest rates of degeneration, regardless of the vitrification time. As a consequence, lower proportions of embryos cleaved in these groups, although differences were eliminated at the five-eight cell stage. Development rates up to day 8 were similar in all experimental groups, being significantly lower than those in fresh controls. Only oocytes vitrified after IVM were able to give blastociysts. The morphological alterations observed can be responsible for compromised development. More research is needed to explain the low survival rates of the bovine oocyte after vitrification and warming. #

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

et al. 2005

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“…The recombinant peptide product of midkine exerts beneficial effects on embryonic development when added during IVM (Ikeda et al 2000a,b), although it is also an intermediary candidate in the induction of developmental alterations (Griffith & Zile 2000). The stimulation by RA at IVM triggers a more physiological cytoplasmic granule migration (Duque et al 2002) and increases oocyte poly-adenylation, suggesting superior mRNA quality and increased processing (Gó mez et al 2004). This coincides with unaltered expression levels of cyclins B1 and H1, specific genes regulating the cell cycle, and less oxidative damage (Gó mez et al 2004).…”

Section: Embryos Transferred Pregnancy (%)mentioning

confidence: 99%

“…The stimulation by RA at IVM triggers a more physiological cytoplasmic granule migration (Duque et al 2002) and increases oocyte poly-adenylation, suggesting superior mRNA quality and increased processing (Gó mez et al 2004). This coincides with unaltered expression levels of cyclins B1 and H1, specific genes regulating the cell cycle, and less oxidative damage (Gó mez et al 2004). Altogether, the above findings correspond to more developmentally competent oocytes (Duque et al 2002.…”

Section: Embryos Transferred Pregnancy (%)mentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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“…Bagavandoss and Midgeley [28] have suggested that RA may stimulate FSH for the induction of LH receptors and also encourage progesterone production and decrease cAMP levels. Cortical granule migration as a maturation clue could be affected by RA since RA improves granular migration [29,30]. Expression of several antioxidant enzymes during in vitro oocyte maturation activates several internal defense mechanisms that protect oocytes against reactive oxygen species (ROS) [25].…”

Section: Discussionmentioning

confidence: 99%

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

Tahaei

Eimani

Eftekhari‐Yazdi

et al. 2011

J Assist Reprod Genet

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Purpose Evaluation of the all-trans retinoic acid (t-RA) effects on in vitro maturation (IVM) and in vitro fertilization (IVF) of immature mouse oocytes in the presence and absence of granulosa cell monolayer. Methods Denuded oocytes isolated from mice ovaries and matured in IVM medium alone (Control I), IVM medium in the presence of granulosa cells (Control II), IVM medium with t-RA (Experimental I) and IVM medium simultaneously with t-RA and granulosa cells (Experimental II). After 24 h, matured oocytes were fertilized in T6 medium and their development was followed until the blastocyst stage. Metaphase II oocytes ploidy were evaluated by chromosome counting. Results The t-RA group compared to the control groups showed no obvious abnormalities. Additionally maturation and embryo development rates significantly increased in the t-RA treated granulosa cell co-culture system. Conclusions In conclusion, association of t-RA with granulosa cell co-culture during in vitro maturation increases meiosis resumption, formation of metaphase II oocytes, as well as 2-cell and blastocyst stage embryos.

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Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Cited by 30 publications

References 54 publications

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Effects of retinoic acid on maturation of immature mouse oocytes in the presence and absence of a granulosa cell co-culture system

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