RETRACTED ARTICLE: An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications

Satpati, Gour Gopal; Mallick, Sourav; Pal, Ruma

doi:10.1007/s12257-015-0281-z

Cited by 18 publications

(10 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although GC–MS provide precise analysis of lipid composition, it cannot provide the detailed information of lipid metabolism. Vital staining methods using Nile Red or BODIPY 505/515 can show lipid distribution in a single cell but cannot provide some desired lipid characteristics such as chain length and degree of unsaturation [ 5 , 6 ]. Studies showed that the lipid content of one cell was significantly correlated with the fluorescence intensity of Nile Red combined with the lipid inside the cell [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy

Shao

Fang

Zhou

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

In this study, confocal Raman microspectroscopy was used to detect lipids in microalgae rapidly and non-destructively. Microalgae cells were cultured under nitrogen deficiency. The accumulation of lipids in Scenedesmus obliquus was observed by Nile red staining, and the total amount of lipids accumulated in the cells was measured by gravimetric method. The signals from different microalgae cells were collected by confocal Raman microspectroscopy to establish a prediction model of intracellular lipid content, and surface scanning signals for drawing pseudo color images of lipids distribution. The images can show the location of pyrenoid and lipid accumulation in cells. Analyze Raman spectrum data and build PCA-LDA model using four different bands (full bands, pigments, lipids, and mixed features). Models of full bands or pigment characteristic bands were capable of identifying S. obliquus cells under different nitrogen stress culture time. The prediction accuracy of model of lipid characteristic bands is relatively low. The correlation between the fatty acid content measured by the gravimetric method and the integral Raman intensity of the oil characteristic peak (1445 cm−1) measured by Raman spectroscopy was analyzed. There was significant correlation (R 2 = 0.83), which means that Raman spectroscopy is applicable to semi-quantitative detection of microalgal lipid content.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy

Shao

Fang

Zhou

et al. 2017

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…In our previous study, marine macroalga Rhizoclonium africanum showed maximum growth and lipid production at limited nitrogen [13]. Most of the works on biomass and lipid accumulation had been done using unicellular microalga [6,[28][29][30][31][32], while very few reports are available on filamentous macroalga [12,14,33]. In the present study different concentrations of four nutrients like nitrogen, phosphorus, EDTA and NaCl were introduced in the culture medium to study the growth pattern in terms of biomass yield and lipid accumulation in marine macroalga C. aerea.…”

Section: Discussionmentioning

confidence: 99%

Effects of nitrogen, phosphorus, EDTA and sodium chloride on biomass and lipid accumulation of Chaetomorpha aerea

Satpati¹,

Pal²

2020

Self Cite

View full text Add to dashboard Cite

The increase of total lipid and fatty acids production was studied under different nutrient stress conditions using the macroalga, Chaetomorpha aerea. The effects of nitrogen, phosphorus, ethylene diamine tetra-acetate and sodium chloride on the growth and lipid accumulation were systematically investigated in laboratory conditions. The biomass was harvested at different stages of cultivation and assessed. The maximum changes of growth and lipid accumulation were observed in the exponential phase at different cultural conditions. The two-fold increase of total lipid was found in the order of 28.27±0.04 % (at 0.1 g/L nitrogen) > 27.30±0.37 % (at 0.5 g/L of phosphorus) > 25.86±0.77 % (at 0.05 g/L of EDTA)> 24.37±0.04 % (at 0.05 g/L NaCl) on 8th day of cultivation. The fatty acids were identified and quantified by gas chromatography mass spectrometry (GC-MS). The alga produces significantly high amount of monounsaturated fatty acid (MUFA) and saturated fatty acids (SFA) than the polyunsaturated fatty acids (PUFA) in different cultural conditions. The elevated levels of C16:1, C18:1 and C20:1 was identified under nitrate, phosphate and salt stress conditions, which are more suitable for biodiesel production.

show abstract

“…However, the conventional methods used for the quantitative analysis of microalgae lipids, e.g., gas chromatography–mass spectrometry (GC–MS) can accurately analyze lipid components, but the detection is time-consuming, and the sample preparation process is complicated, in the meantime it cannot analyze the lipid metabolism process in cells. The other technology, Nile red (NR) or BODIPY 505/515 for instance can show lipid distribution in cells, but the fluorescence intensity is affected by many factors, such as emission wavelength, dye concentration, cell density and staining time [ 7 – 9 ]. Therefore, it is necessary to find a fast and non-destructive method for detecting lipid content.…”

Section: Introductionmentioning

confidence: 99%

Lipids monitoring in Scenedesmus obliquus based on terahertz technology

Shao

Qiu

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Microalgae are considered as a source of low pollution and renewable fuel due to their ability to synthesize an abundance of lipids. Conventional methods for lipid quantification are time-consuming and chemically contaminated, while spectroscopic method combined with mathematical model is much more attractive due to its ability of qualitative and quantitative analysis of material composition, in this sense, terahertz technology provides not only timely and non-destructive testing without chemical pollution, but also provides information on the functional group vibration mode and structure of the measured components. Therefore, terahertz technology is utilized in our investigation and proposed for microalgae metabolism detection. Results The aim of this study was to use terahertz spectroscopy to observe lipid content in Scenedesmus obliquus (S. obliquus). We collected the THz spectra of S. obliquus which were cultivated under nitrogen stress and terahertz spectroscopy was used to analyze changes in substance components (lipids, proteins, carbohydrates and β-carotene). The PLS algorithm was used to model the terahertz data to distinguish the different lipid content of S. obliquus under nitrogen stress. The correlation coefficient of the prediction results of the lipid characteristic band modeling was above 0.991, and the root mean square error was less than 0.132. It indicated that terahertz technology can be used to discriminate S. obliquus cells under different nitrogen stress effectively. The correlation between the terahertz characteristic peak (9.3 THz) and the total lipid content determined by gravimetry reaches 0.960. The final results were compared with the commonly used spectroscopic methods for lipid observation (Raman spectroscopy). Conclusions In this article, we demonstrated the effectiveness of terahertz spectroscopy to monitor changes in microalgae lipid content under nitrogen stress. Terahertz spectroscopy is more suitable for industrial production or ordinary laboratories which require intermediate result with low-frequency screening. When quantifying microalgae lipids, the constraint of terahertz spectroscopy is far less than that of Raman spectroscopy, and it is easier for operator to accurately quantify microalgae lipid. In addition, it is still in early stage for the study of microalgae using terahertz spectroscopy technology, there is still much potential for us to explore.

show abstract

RETRACTED ARTICLE: An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications

Cited by 18 publications

References 58 publications

Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy

Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy

Effects of nitrogen, phosphorus, EDTA and sodium chloride on biomass and lipid accumulation of Chaetomorpha aerea

Lipids monitoring in Scenedesmus obliquus based on terahertz technology

Contact Info

Product

Resources

About