RETRACTED ARTICLE: Astragalus polysaccharide alleviates H ₂ O ₂ -triggered oxidative injury in human umbilical vein endothelial cells via promoting KLF2

Abstract

The damage of vascular endothelial cells has become an indispensable factor in the occurrence and advancement of cardiovascular diseases. In the current study, we investigated the effect of Astragalus Polysacharin (APS) on H 2 O 2-evoked oxidative injury in HUVECs. HUVECs cells were treated by H 2 O 2 to induce oxidative damage. Cells viability, apoptosis and reactive oxygen species (ROS) level were detected through CCK8 assay and flow cytometry. The cell growth-related proteins and heme oxygenase-1(HO-1) and KLF2 expression were evaluated via Western blot assay. The functions of KLF2 in APS and H 2 O 2 co-disposed HUVECs were explored after si-KLF2 transfection. MEK/ERK pathway was finally measured through Western blot. We found that H 2 O 2 stimulation-evoked HUVECs oxidative damage meanwhile impeded HO-1 expression. APS treatment effectively suppressed H 2 O 2-induced oxidative injury in HUVECs. KLF2 and Nrf2 expression were elevated by APS and KLF2 repression abolished the protective action of APS in H 2 O 2-triggered cell injury. MEK/ERK pathway was activated by APS treatment. Furthermore, the MEK/ERK pathway inhibitor weakened the promoting effect of APS on the expression of KLF2. In conclusion, our study reveals that APS alleviates H 2 O 2-triggered oxidative injury in HUVECs via elevating the expression of KLF2 via the MEK/ERK pathway.

Activation of Nrf2/HO-1 signaling: An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress

“…Astragalus polysaccharide (ASTP) reduces ROS generation and the expression of Baz, Caspase-9 and Caspase-3, increases HO-1 and Nrf2 expression, inhibits cell apoptosis, and effectively protects HUVECs from H 2 O 2 stimulation. KLF2, a transcription regulatory protein, primes Nrf2 for activation in endothelial cells, and the authors also found that ASTP can increase KLF2 expression [80] . Similarly, polysaccharides from Athyrium multidentatum (AMP) increase the survival rate of H 2 O 2 -damaged HUVECs, reduce ROS production, and enhance SOD and CAT activity.…”

Astragalus polysacharin inhibits hepatocellular carcinoma-like phenotypes in a murine HCC model through repression of M2 polarization of tumour-associated macrophages

“…For instance, APS protects human umbilical vein endothelial cells from oxidative damage triggered by H 2 O 2 through promoting the expression of KLF2, thereby reducing the advancement of cardiovascular diseases (Li et al. 2019 ). APS also has antitumor effects in various cancers.…”

“… 2013 ; Li et al. 2019 ). Therefore, this study hypothesized that APS partakes in polarization of TAMs in HCC in order to inhibit HCC-like phenotypes including cell proliferation, migration and invasion.…”

Biological and Cytoprotective Effect of Piper kadsura Ohwi against Hydrogen-Peroxide-Induced Oxidative Stress in Human SW1353 Cells

“…Through the addition of inhibitors, it is found that all of the MAPKs are the key to regulation. The three pathways of JNK [ 1 ], MEK/ERK [ 31 ], and p38 [ 32 ] are regulated by regulating GPx, CAT, and SOD-2 in cells to protect the regulation of Bcl-2 and Bax on the mitochondrial membrane. PKE was found to promote cell proliferation in the MTT test, and it was confirmed in the results of the cell morphology observation and cell apoptosis analysis.…”