RETRACTED ARTICLE: Circ_0017639 facilitates proliferative, migratory, and invasive potential of non-small cell lung cancer (NSCLC) cells via PI3K/AKT signaling pathway

Abstract

Non-small cell lung cancer (NSCLC) has increased morbidity and mortality rate worldwide. The current NSCLS therapies are associated with poor outcomes and need further improvement. CircRNAs were shown to regulate NSCLC progression. However, little is known re garding the functions and mechanisms of circ_0017639 in NSCLC, which requires further extensive studies. The circ_0017639 expression in NSCLC tissues and cell lines was evaluated via qRT-RCR. Moreover, using ectopic plasmid incorporation and shRNA assays, we analyzed the circ_0017639-mediated cellular proliferative, migratory and invasive processes in NSCLC cell lines, using CCK-8, EdU, and transwell assays. Furthermore, the core proteins (p-PI3K, PI3K, p-AKT, and AKT) levels of the PI3K/AKT signaling cascade were investigated via immunoblotting. Finally, we tested the functional role of circ_0017639 by examining its regulation of xenograft tumor growths in nude mice in vivo . Circ_0017639 expression was remarkably high in the NSCLC tissues and cell lines. The transfection experiments showed that circ_0017639 overexpression was able to promote proliferative, migratory, and invasive properties of NSCLC cells, while sh-circ_0017639 showed opposing effects. We further showed that circ_0017639 knockdown suppressed the cellular development via PI3K/AKT cascade inactivation. Additionally, in-vivo experiment in nude mice demonstrated that sh-circ_0017639 could reduce the tumor growth of NSCLC. Circ_0017639 may promote the development of NSCLC by accelerating NSCLC metastasis through stimulating the PI3K/AKT cascade.

Exploration of Ginkgo biloba leaves on non-small cell lung cancer based on network pharmacology and molecular docking

“…[43] Kaempferol, a flavonoid from natural plant sources, has proved to be effective in anti-NSCLC, specifically in inducing apoptosis in NSCLC cells, [44] promoting autophagy in NSCLC cells, [45] increasing the sensitivity to radiotherapy, [46] and inhibiting migration of A549 cells. [47] The PI3K-AKT signaling pathway is a key signaling pathway that regulates the progression of metastasis, [48] cell proliferation, differentiation, and metabolism. [49] It has been shown that some natural substances like Fucoxanthin could inhibit proliferation, and arrest cell cycle at the G0/G1 phase in NSCLC cells through the PI3K-AKT signaling pathway.…”

“…The PI3K-AKT signaling pathway is a key signaling pathway that regulates the progression of metastasis, [48] cell proliferation, differentiation, and metabolism. [49] It has been shown that some natural substances like Fucoxanthin could inhibit proliferation, and arrest cell cycle at the G0/G1 phase in NSCLC cells through the PI3K-AKT signaling pathway.…”

Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer

“…Nevertheless, circ_0017639 exerted an inhibiting impact on glioma cell metastasis and proliferation, which might be related to the tissue-specific expression [ 51 ]. A recent report showed that circ_0017639 was highly expressed in NSCLC samples and cell lines, and circ_0017639 overexpression promoted NSCLC cell proliferation, invasion, and migration via the PI3K/AKT signaling [ 52 ]. The difference between our study and the previous report [ 52 ] was to explore the function of circ_0017639 in DDP resistance in NSCLC.…”

“…A recent report showed that circ_0017639 was highly expressed in NSCLC samples and cell lines, and circ_0017639 overexpression promoted NSCLC cell proliferation, invasion, and migration via the PI3K/AKT signaling [ 52 ]. The difference between our study and the previous report [ 52 ] was to explore the function of circ_0017639 in DDP resistance in NSCLC. Our data showed higher levels of circ_0017639 in DDP-resistant samples and cell lines.…”

The emerging role of circular RNAs in drug resistance of non-small cell lung cancer

“…For example, circ_CDR1-AS contributes to resistance to pemetrexed and CDDP through activating the EGFR/PI3K pathway in LUAD ( 88 ). Additionally, circ_0017639 ( 89 ) and circ_0008594 ( 90 ) both can facilitate the progression of NSCLC by PI3K signaling pathway. Therefore, circRNAs are potential targets for intervening in drug resistance problems, with circRNAs playing a significant role in the modulation of the PI3K/AKT pathway.…”