RETRACTED ARTICLE: Circular RNA ABCB10 promotes hepatocellular
carcinoma progression by increasing HMG20A expression by sponging
miR-670-3p

Fu, Yu; Cai, Limin; Lei, Xuexue; Wang, Dunwei

doi:10.1186/s12935-019-1055-z

Cited by 23 publications

(11 citation statements)

References 35 publications

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“…13,14 For example, circABCB10 could promote HMG20A expression to enhance the proliferation and invasion of HCC by sponging miR-670-3p. 15 Also, circ_0001955 could accelerate the tumorigenesis of HCC via regulating the miR-516a-5p/TRAF6-MAPK11 network. 16 Therefore, circRNA may be an important biomarker for cancer therapy and may provide an important basis for elucidating the pathogenesis of cancers.…”

Section: Introductionmentioning

confidence: 99%

<p>Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network</p>

Fang¹,

Liu²,

Zhou³

et al. 2020

CMAR

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Background Many studies have confirmed that circular RNAs (circRNAs) play a key role in the biological progression of cancers. However, the function of a novel circRNA, circ_0046599, in hepatocellular carcinoma (HCC) progression has not been explored. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to measure the expression of circ_0046599, microRNA (miR)-1258 and Ribophorin II (RPN2). Subcellular fractionation location assay was used to localize circ_0046599 in HCC cells. The circular characteristic of circ_0046599 was verified using Ribonuclease R (RNase R) digestion assay. Besides, cell counting kit 8 (CCK8) assay, colony formation assay, wound healing assay and transwell assay were used to detect cell proliferation, migration and invasion, respectively. The lactate production and glucose level were determined by Lactate and Glucose Assay Kits. Furthermore, the protein levels of glycolysis, metastasis and proliferation-related marker proteins, as well as RPN2 were tested by Western blot (WB) analysis. Moreover, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to confirm the interactions among circ_0046599, miR-1258 and RPN2. In addition, mice xenograft models were applied to observe the effect of circ_0046599 silencing on HCC tumor growth in vivo. Results Circ_0046599 was highly expressed in HCC tissues and cells, and its knockdown could suppress HCC cell proliferation, migration, invasion and glycolysis process. MiR-1258 could be targeted by circ_0046599, and its inhibitor could invert the suppressing effect of circ_0046599 knockdown on HCC progression. Further, RPN2 was a target of miR-1258. Overexpressed RPN2 could reverse the inhibiting effect of miR-1258 overexpression on HCC progression. Also, knockdown of circ_0046599 could restrain HCC tumor growth in vivo. Conclusion Our results provided new evidence that circ_0046599 could promote the progression of HCC by increasing RPN2 expression via sponging miR-1258.

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Section: Introductionmentioning

confidence: 99%

<p>Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network</p>

Fang¹,

Liu²,

Zhou³

et al. 2020

CMAR

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“…The Editor-in-Chief has retracted this article [1] due to lack of evidence that the study has received ethics approval. After publication it has come to the Editor's attention that in the body of the article the authors state that their study was approved by the First Hospital of Jilin University Research Ethics Committee.…”

mentioning

confidence: 99%

Retraction Note to: Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p

Cai

Lei

et al. 2020

Cancer Cell Int

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“…In another case, an insertion at chr1:228921863, located in a super-enhancer, was observed to be associated with increased expression of ABCB10 gene (corrected p-value = 3.7⋅10 − 3 ) and in the same causal set with rs61824911, a GWAS susceptibility locus for basal cell carcinoma (BCC) (Chahal et al 2016). Multiple studies have reported the gene ABCB10 as a susceptibility locus for different types of cancers, such as breast cancer, clear cell renal cell carcinoma, hepatocellular carcinoma, and epithelial ovarian cancer (Liang et al 2017;Chen et al 2019;Huang et al 2019;Fu et al 2019), and its increased expression has been reported in a retinoblastoma cell line (Hendig et al 2009). Future studies on SVs can contribute to deeper understanding on mechanism of disease.…”

Section: Discussionmentioning

confidence: 96%

Characterization of intermediate-sized insertions using whole genome sequencing data and analysis of their functional impacts on gene expression

Ashouri

Wong

Nakagawa

et al. 2021

Preprint

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Intermediate-sized insertions are one of the structural variants contributing to genome diversity. However, due to technical difficulties in identifying them, their importance in disease pathogenicity and gene expression regulation remains unclear. We used whole-genome sequencing data of 174 Japanese samples to characterize intermediate-sized insertions using a highly-accurate insertion calling method (IMSindel software and joint-call recovery) and obtained a catalogue of 4,254 insertions. We constructed an imputation panel comprising of insertions and SNVs from all samples, and conducted imputation of intermediate-sized insertions for 82 publicly-available Japanese samples. Imputation accuracy, evaluated using Nanopore long-read sequencing data, was 97%. Subsequent eQTL analysis predicted 128 (~ 3.0%) insertions as causative for gene expression level changes. Enrichment analysis of causal insertions for genome regulatory elements showed significant associations with CTCF-binding sites, super-enhancers, and promoters. Among 17 causal insertions found in the same causal set with GWAS hits, there were insertions associated with changes in expression of cancer-related genes such as BRCA1, ZNF222, and ABCB10. Analysis of insertions sequences revealed that 461 insertions were short tandem duplications frequently found in early replicating regions of genome. Furthermore, comparison of functional importance of intermediate-sized insertions with that of intermediate-sized deletions detected in the same sample set in our previous study showed that insertions were more frequent in genic regions, and proportion of functional candidates was smaller in insertions. Here, we characterize a high-confidence set of intermediate-sized insertions and indicate their importance in gene expression regulation. Our results emphasize the importance of considering intermediate-sized insertions in trait association studies.

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RETRACTED ARTICLE: Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p

Cited by 23 publications

References 35 publications

<p>Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network</p>

<p>Circ_0046599 Promotes the Development of Hepatocellular Carcinoma by Regulating the miR-1258/RPN2 Network</p>

Retraction Note to: Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p

Characterization of intermediate-sized insertions using whole genome sequencing data and analysis of their functional impacts on gene expression

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