RETRACTED ARTICLE: Downregulating lncRNA NEAT1 induces proliferation and represses apoptosis of ovarian granulosa cells in polycystic ovary syndrome via microRNA-381/IGF1 axis

Abstract

Objective Researchers have revealed the combined functions of long noncoding RNAs (lncRNAs) and microRNA (miRNAs) in polycystic ovary syndrome (PCOS). This study aimed to understand the role of nuclear-enriched abundant transcript 1 (NEAT1) and miR-381 involving insulin-like growth factor 1 (IGF1) in PCOS. Methods PCOS rat model was established by dehydroepiandrosterone induction. NEAT1, miR-381 and IGF1 expression in ovarian granulosa cells of PCOS patients and ovarian tissues of PCOS rats were tested. Bioinformatics website and dual luciferase reporter gene assay were utilized to verify the relationship between NEAT1 and miR-381 and that between miR-381 and IGF1. Levels of sex hormone, pathological changes and ovarian granulosa cell apoptosis in ovarian tissues of PCOS rats were detected. Ovarian granulosa cell proliferation and apoptosis were analyzed in vitro. Results NEAT1 and IGF1 expression increased while miR-381 expression decreased in the ovarian granulosa cells of patients with PCOS and the ovarian tissues of PCOS rats. In in vivo experiments, interference with NEAT1 improved the levels of sex hormones, alleviated pathological changes and suppressed ovarian granulosa cell apoptosis in the ovarian tissues of PCOS rats. In in vitro cell experiments, interference with NEAT1 suppressed apoptosis and enhanced cell proliferation of ovarian granulosa cells. NEAT1 interference-mediated effect would be reversed by up-regulating miR-381. NEAT1 acted as a ceRNA to adsorb miR-381 to target IGF1. Overexpression of IGF1 reversed the inhibitory effect of miR-381 on ovarian granulosa cell apoptosis. Conclusion Interference with NEAT1 increases miR-381 and reduces IGF1 levels, effectively improving the levels of sex hormones and reducing the pathological damage of ovarian tissue in rats with PCOS.

lncRNA DARS-AS1 Modulates TSPAN1-Mediated ITGA2 Hypomethylation by Interaction with miR-194-5p Thus Promoting Ovarian Cancer Progression

“…OC is responsible for the leading cause of cancer-related deaths, with approximately 45% of five-year overall survival [ 27 , 28 ]. As reported in numerous studies, lncRNAs have been involved in cell growth, apoptosis, invasion, and metastasis of malignant tumor including OC [ 29 , 30 ] and produced an effect on the overall survival [ 31 ]. For instance, the lncRNA-mediated m6A regulation was reported to be associated with OC remission and deterioration.…”

High-Throughput Sequencing Profiles About lncRNAs and mRNAs of Ovarian Granulosa Cells in Polycystic Ovary Syndrome

“…And Yang et al showed that silencing the lncRNA UCA1 could inhibit the development of PCOS via regulating PI3K-AKT signaling pathway ( 44 ). Moreover, downregulating lncRNA NEAT1 could also inhibit apoptosis and improve cell proliferation of ovarian GCs via microRNA-381/IGF1 axis, which associated with improved pathological process of PCOS ( 45 ). And our results exhibited that the expression of lncRNA was also significantly correlated with abnormal hormone, plasma metabolites level and embryo quality: NONHSAT101926.2 was mainly related to lipid metabolism, NONHSAT227177.1 and NONHSAT230904.1 were mainly related to plasma steroid hormones, NONHSAT010538.2 was positively associated with the LH/FSH ratio, while ENST00000607307 and NONHSAT191377.1 were related to plasma steroid hormones and follicle development.…”

LncRNA NEAT1 facilitates glioma progression via stabilizing PGK1

“…Total RNA from U251 and T98G cells was extracted using TRIzol reagent (Invitrogen, CA) as previously described [ 15 ]. qRT-PCR was performed using SYBR Green Premix Ex Taq (TaKaRa) according to the manufacturer’s recommendations.…”

“…Protein samples preparation and western blot analysis were performed as previously described [ 15 ]. Briefly, glioma cells were lysed using RIPA lysis buffer supplemented with protease inhibitors.…”