RETRACTED ARTICLE: Effect of the co-culture of human bone marrow mesenchymal stromal cells with human umbilical vein endothelial cells in vitro

Abstract

Mesenchymal stem cells (MSCs) give origin to the marrow tromal environment that supports hematopoiesis. These cells present a wide range of differentiation potentials and a complex relationship with hematopoietic stem cells (HSCs) and endothelial cells. In addition to bone marrow (BM), MSCs can be obtained from other sites in the adult or the fetus. Recent studies have shown that cocultured endothelial cells and osteoblasts are mutually promotive in bone tissues repair. In this study, we observed the effects of coculture of endothelial cells and osteoblasts at different ratios on vasculogenesis and bone formation, and we found that angiogenic effect is more effective when endothelial cells are cocultured with osteoblasts at the ratio of 4:1, and osteogenic effect is more effective at the ratio of 1:4. It is concluded that the co-culture of human bone marrow mesenchymal stromal cells with human umbilical vein endothelial cells could be a promising culture system for bone tissue engineering applications.

Gene expression in human adipose-derived stem cells: comparison of 2D films, 3D electrospun meshes or co-cultured scaffolds with two-way paracrine effects

“…When using stem cells for fabrication of an implant, a central question is how to trigger their differentiation towards a specific cell type. Adding supplements to the culture media (Zuk et al, 2001) or co-culturing with a different cell type (Lin et al, 2016) are well known options to support differentiation towards various cell fates. Establishment of spheroids (Emmert et al, 2013;Kapur et al, 2012) or cell seeding on posts using two-dimensional (2D) micro-engineering (Li and Kilian, 2015) may also act as appropriate strategies.…”

Co‐transplantation of Wharton's jelly mesenchymal stem cell‐derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

“…The ratio between the co‐cultured cells (1:1 in our study) might also influence angiogenesis or osteogenesis in a significant manner. Lin et al () demonstrated that osteogenic formation is more effective when ECs are co‐cultured with OBs at a ratio of 1:4 and that angiogenesis is more effective at a ratio of 4:1. Another in vitro study demonstrated that a ratio of 5:1 improved vasculogenesis, and a ratio of 1:5 increased mineralization (Shah, Wenke, & Agrawal, ).…”

Directing Stem Cell Commitment by Amorphous Calcium Phosphate Nanoparticles Incorporated in PLGA: Relevance of the Free Calcium Ion Concentration

“…Supplements, such as calcium ions [1], bone morphogenetic proteins (BMPs) [2], dexamethasone [3] and bioactive peptides [4,5] in the culture media of stem cells trigger the intended differentiation towards the osteoblast phenotype [6]. Apart from adding supplements, co-cultures [7], microtissues [8] or mechanical stimulation [9][10][11] are promising approaches in this regard. Moreover, the composition of a material acting as a scaffold [12], its surface properties [13], pore size and pore connectivity [14] are essential for the specific commitment of stem cells.…”