RETRACTED ARTICLE: Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16

Abstract

Background: Glioma is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of glioma cells remains elusive. Methods: Sevoflurane (Sev), a class of common anesthetics, was used to expose to U87-MG and U251 cells. The expressions of microRNA-146b-5p (miR-146b-5p) and matrix metallopeptidase 16 (MMP16)were measured by quantitative real-time polymerase chain reaction or western blot. Transfection was performed in glioma cells with miR-146b-5p inhibitor, inhibitor negative control, MMP16 overexpression vector, empty vector, small interfering RNA against MMP16 or scramble. Cell migration and invasion were analyzed by the trans-well assay. The interaction between miR-146b-5p and MMP16 was explored by luciferase activity and RNA immunoprecipitation assays. Results: Sev treatment inhibited migration and invasion of glioma cells. The expression of miR-146b-5p was enhanced and MMP16 protein was decreased in glioma cells after exposure of Sev. Knockdown of miR-146b-5p or overexpression of MMP16 reversed Sev-induced inhibition of migration and invasion of glioma cells. Moreover, MMP16 was indicated as a target of miR-146b-5p and its silencing attenuated the regulatory role of miR-146b-5p abrogationin Sev-treated glioma cells. Conclusion: Sev impeded cell migration and invasion through regulating miR-146b-5p and MMP16 in glioma, indicating a novel theories foundation for the application of anesthetics like Sev in glioma.

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

“…Glioma is an aggressive tumor and the average survival time in patients with high-grade glioma is not more than one year. [27][28][29][30] Sev was reported to be involved in the regulation of glioma progression 4,31 and our data showed that Sev inhibited glioma progression in vitro and tumor growth in vivo. However, the current studies were mostly focused on the regulatory mechanisms of miRNAs and long noncoding RNAs in Sev-mediated human diseases.…”

“…For Sev treatment, cells were first treated with various concentrations of Sev (1.7%, 3.4% and 5.1%) for 6 h and then the cells were normally cultured for 24 h for further investigation according to a previous report. 4…”

“…3 Sevoflurane (Sev), a class of common anesthetics, was reported to inhibit migration and invasion of glioma cells. 4 However, the regulatory mechanism of Sev in glioma remains poorly understood.…”

<p>microRNA-26a Directly Targeting MMP14 and MMP16 Inhibits the Cancer Cell Proliferation, Migration and Invasion in Cutaneous Squamous Cell Carcinoma</p>

“…A431 and SCL-1 cells were transfected with 50nM oligonucleotides or vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. AgomiR-26a, miR-26a inhibitor, pcDNA3.1-MMP14/ MMP16 overexpression vector (MMP14/MMP16) 17,21,22 were synthesized by Genepharma (Shanghai, China).…”

Bupivacaine suppresses the progression of gastric cancer through regulating circ_0000376/miR-145-5p axis

“…For example, Propofol impeded the proliferation and motility of gastric cancer cells through targeting miR-29/MMP2 signaling [ 27 ]. Sevoflurane restrained the metastasis of glioma cells through modulating miR-146b-5p/MMP16 axis [ 28 ]. Zhang et al found that Bupivacaine induced the apoptosis and neurotoxicity of neuroblastoma cells through regulating miR-132/IGF1R axis [ 29 ].…”