RETRACTED: Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells

Ozeki, Nobuaki; Mogi, Makio; Hase, Naoko; Hiyama, Taiki; Yamaguchi, Hideyuki; Kawai, Rie; Matsumoto, Toru; Nakata, Kohei

doi:10.1016/j.yexcr.2016.07.002

Cited by 22 publications

(27 citation statements)

References 34 publications

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“…BMP signaling plays a significant role in controlling various cellular processes including autophagy and differentiation . Recently, it has been demonstrated that BMP‐2‐induced differentiation in human stem cell‐derived osteoblast cells involves autophagy protein Atg7 .…”

Section: Discussionmentioning

confidence: 99%

“…The treated cells were lysed and equal amounts of protein were mixed with reaction buffer, followed by mixing with pNPP solution and equilibration at 37°C. The absorbance was measured at 450 nm, immediately …”

Section: Methodsmentioning

confidence: 99%

“…Imaging was done using a high‐end fluorescence inverted microscope (Olympus IX71), using the cellSens Standard software. Colocalization was measured by applying JACoP plugin in single Z‐stack sections of deconvoluted images …”

Section: Methodsmentioning

confidence: 99%

“…Using the protein‐protein docking algorithms, the optimal orientation of the two proteins could be identified by scoring the energy of the protein‐protein complex on the basis of van der Waals (VDW) interactions and the corresponding electrostatics. Therefore, the grid‐based score was generated by calculating the nonbonded terms of the molecular mechanics force field, and the structure with the highest score was then considered for MD simulation …”

Section: Methodsmentioning

confidence: 99%

“…Further, BMP‐2 has been demonstrated to induce chondrogenic differentiation in vitro, through down‐regulation of membrane‐bound β‐catenin . Recently, it has been demonstrated that the expression of Atg7 and the Wnt signaling were elevated during BMP‐2‐mediated human osteoblast differentiation . Reports have documented that BMP pathways are inactivated in the majority of the sporadic CRCs .…”

Section: Introductionmentioning

confidence: 99%

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Abrus agglutinin stimulates BMP‐2‐dependent differentiation through autophagic degradation of β‐catenin in colon cancer stem cells

Panda

Naik

Praharaj

et al. 2018

Molecular Carcinogenesis

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Eradicating cancer stem cells (CSCs) in colorectal cancer (CRC) through differentiation therapy is a promising approach for cancer treatment. Our retrospective tumor-specimen analysis elucidated alteration in the expression of bone morphogenetic protein 2 (BMP-2) and β-catenin during the colon cancer progression, indicating that their possible intervention through "forced differentiation" in colon cancer remission. We reveal that Abrus agglutinin (AGG) induces the colon CSCs differentiation, and enhances sensitivity to the anticancer therapeutics. The low dose AGG (max. dose = 100 ng/mL) decreased the expression of stemness-associated molecules such as CD44 and β-catenin in the HT-29 cell derived colonospheres. Further, AGG augmented colonosphere differentiation, as demonstrated by the enhanced CK20/CK7 expression ratio and induced alkaline phosphatase activity. Interestingly, the AGG-induced expression of BMP-2 and the AGG-induced differentiation were demonstrated to be critically dependent on BMP-2 in the colonospheres. Similarly, autophagy-induction by AGG was associated with colonosphere differentiation and the gene silencing of BMP-2 led to the reduced accumulation of LC3-II, suggesting that AGG-induced autophagy is dependent on BMP-2. Furthermore, hVps34 binds strongly to BMP-2, indicating a possible association of BMP-2 with the process of autophagy. Moreover, the reduction in the self-renewal capacity of the colonospheres was associated with AGG-augmented autophagic degradation of β-catenin through an interaction with the autophagy adaptor protein p62. In the subcutaneous HT-29 xenograft model, AGG profoundly inhibited the growth of tumors through an increase in BMP-2 expression and LC3-II puncta, and a decrease in β-catenin expression, confirming the antitumor potential of AGG through induction of differentiation in colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Abrus agglutinin stimulates BMP‐2‐dependent differentiation through autophagic degradation of β‐catenin in colon cancer stem cells

Panda

Naik

Praharaj

et al. 2018

Molecular Carcinogenesis

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Osteoblast autophagy in glucocorticoid‐induced osteoporosis

Wang

Heckmann

Yang

et al. 2018

Journal Cellular Physiology

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Administration of glucocorticoids is an effective strategy for treating many inflammatory and autoimmune diseases. However, glucocorticoid treatment can have adverse effects on bone, leading to glucocorticoid-induced osteoporosis (GIO), the most common form of secondary osteoporosis. Although the pathogenesis of GIO has been studied for decades, over the past ten years the autophagy machinery has been implicated as a novel mechanism. Autophagy in osteoblasts, osteocytes, and osteoclasts plays a critical role in the maintenance of bone homeostasis. Herein, we specifically discuss how osteoblast autophagy responds to glucocorticoids and its role in the development of GIO. K E Y W O R D S autophagy, glucocorticoid-induced osteoporosis, osteoblasts

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Autophagic Mediators in Bone Marrow Niche Homeostasis

Agas

Sabbieti

2021

Advances in Experimental Medicine and Biology

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RETRACTED: Bone morphogenetic protein-induced cell differentiation involves Atg7 and Wnt16 sequentially in human stem cell-derived osteoblastic cells

Cited by 22 publications

References 34 publications

Abrus agglutinin stimulates BMP‐2‐dependent differentiation through autophagic degradation of β‐catenin in colon cancer stem cells

Abrus agglutinin stimulates BMP‐2‐dependent differentiation through autophagic degradation of β‐catenin in colon cancer stem cells

Osteoblast autophagy in glucocorticoid‐induced osteoporosis

Autophagic Mediators in Bone Marrow Niche Homeostasis

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