[Retracted] Interfering with pak4 Protein Expression Affects Osteosarcoma Cell Proliferation and Migration

Abstract

Purpose. A number of studies have discovered various roles of PAK4 in human tumors, including osteosarcoma. However, the exact role of PAK4 in osteosarcoma and its mechanism have yet to be determined. Therefore, this study focused on interrogating the PAK4 effect on the proliferation and migration ability of osteosarcoma and its underlying mechanisms. Materials and Methods. Western blot and QRT-PCR were utilized to quantify the PAK4 relative protein and mRNA levels. To measure cellular viability and mobility, the MTT and wound-healing assays were preferred. Results. With the adenovirus-mediated overexpression of PAK4, the proliferation and migration of U2-OS and MG-63 osteosarcoma cells were stimulated. Furthermore, a liposome-mediated knockout of PAK4 will inhibit osteosarcoma cells from proliferating. In terms of mechanism, we observed the positive correlation of PAK4 expression with expression of P21, CyclinD1, CyclinE1, CDK2, and CDK6, which drives G0/G1 to the G2/M phase transition. PAK4 can also activate Erk expression in OS cells and induce EMT. Conclusion. Interfering with PAK4 protein expression has been shown to affect osteosarcoma proliferation and migration.

FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma

“…Motion trajectories of U2-OS and MG-63 cells were tracked and imaged using a Holomonitor ® M4 microscope [ 15 ]. Open the microscope component of the Holomonitor ® M4 system and place it in the CO 2 incubator overnight to improve the stability of each parameter.…”

Recent advances on development of p21-activated kinase 4 inhibitors as anti-tumor agents

“…Recently, more and more evidences suggested that PAK4 might be an ideal target for treat certain cancers ( Santiago-Gómez et al, 2019 ; Wang F et al, 2019 ; Dasgupta et al, 2021 ). Inhibition of PAK4 was found to attenuate cancer cell migration and invasion, and suppress growth of diverse tumors in vivo ( Guo et al, 2020 ; Fu et al, 2021 ). Furthermore, PAK4 was also found as an important regulator of tumor immunity ( Nasmall Yi et al, 2021 ), thus opening a novel landscape for PAK4-related therapy.…”

Retracted: Interfering with pak4 Protein Expression Affects Osteosarcoma Cell Proliferation and Migration

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Manipulated or compromised peer review …”

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”