RETRACTED: Knockdown of long non-coding RNA CCAT1 suppresses proliferation and EMT of human cervical cancer cell lines by down-regulating Runx2

Li, Ruiping; Liu, Jinyu; Qi, Jinhong

doi:10.1016/j.yexmp.2020.104380

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…In addition, it promoted CC proliferation, invasion, and epithelial-mesenchymal transformation (EMT) by regulating the miR-503-5p/TRAF4 axis (4). Li et al reported that lncRNA CCAT1 was over-expressed in CC tissues, and CCAT1 knockout restrained CC development via inhibiting cell proliferation, migration, invasion, EMT, and accelerating cell apoptosis (5). On the other side, some lncRNAs, such as lncRNA PTENP1, were reported to inhibit cervical cancer development (6).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway

et al. 2021

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Aims: A growing number of studies have unveiled that long non-coding RNA (lncRNA) is conductive to cervical cancer (CC) development. However, the effect of LIPE-AS1 is remained to be studied in CC.Main Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was employed to measure LIPE-AS1 expression in CC tissues and the adjacent normal tissues. Additionally, we conducted gain- and loss-of functional experiments of LIPE-AS1 and adopted CCK8 assay, BrdU assay, and in vivo tumor formation experiment to test the proliferation of CC cells (HCC94 and HeLa). Besides, the apoptosis, invasion, and epithelial-mesenchymal transformation (EMT) of CC cells were estimated using flow cytometry, transwell assay, and western blot, respectively. Further, LIPE-AS1 downstream targets were analyzed through bioinformatics, and the binding relationships between LIPE-AS1 and miR-195-5p were verified via dual-luciferase activity experiment and RNA Protein Immunoprecipitation (RIP) assay. Moreover, rescue experiments were conducted to confirm the effects of LIPE-AS1 and miR-195-5p in regulating CC development and the expressions of MAPK signaling pathway related proteins were detected by RT-PCR, western blot, and immunofluorescence.Key Findings: LIPE-AS1 was over-expressed in CC tissues (compared to normal adjacent tissues) and was notably related to tumor volume, distant metastasis. Overexpressing LIPE-AS1 accelerated CC cell proliferation, migration and EMT, inhibited apoptosis; while LIPE-AS1 knockdown had the opposite effects. The mechanism studies confirmed that LIPE-AS1 sponges miR-195-5p as a competitive endogenous RNA (ceRNA), which targets the 3′-untranslated region (3′-UTR) of MAP3K8. LIPE-AS1 promoted the expression of MAP3K8 and enhanced ERK1/2 phosphorylation, which were reversed by miR-195-5p.Significance: LIPE-AS1 regulates CC progression through the miR-195-5p/MAPK signaling pathway, providing new hope for CC diagnosis and treatment.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway

et al. 2021

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“…CCAT1 is an oncogenic lncRNA in cervical cancer cells whose silencing has blocked proliferation, migratory potential, invasiveness and EMT process in these cells. CCAT1 silencing has led to down-regulation of Runx2 and suppression of PI3K/AKT signaling in cervical cancer cells PI3K/AKT signal (Li et al, 2020a). HAL is a down-regulated lncRNA in serous ovarian cancer tissues and cells.…”

Section: Sample Numbermentioning

confidence: 99%

The Impact of Non-coding RNAs in the Epithelial to Mesenchymal Transition

Hussen

Shoorei

Mohaqiq

et al. 2021

Front. Mol. Biosci.

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Epithelial to mesenchymal transition (EMT) is a course of action that enables a polarized epithelial cell to undertake numerous biochemical alterations that allow it to adopt features of mesenchymal cells such as high migratory ability, invasive properties, resistance to apoptosis, and importantly higher-order formation of extracellular matrix elements. EMT has important roles in implantation and gastrulation of the embryo, inflammatory reactions and fibrosis, and transformation of cancer cells, their invasiveness and metastatic ability. Regarding the importance of EMT in the invasive progression of cancer, this process has been well studies in in this context. Non-coding RNAs (ncRNAs) have been shown to exert critical function in the regulation of cellular processes that are involved in the EMT. These processes include regulation of some transcription factors namely SNAI1 and SNAI2, ZEB1 and ZEB2, Twist, and E12/E47, modulation of chromatin configuration, alternative splicing, and protein stability and subcellular location of proteins. In the present paper, we describe the influence of ncRNAs including microRNAs and long non-coding RNAs in the EMT process and their application as biomarkers for this process and cancer progression and their potential as therapeutic targets.

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“…Previous studies have also discovered the close relationship between RUNX2 and malignant phenotypes of other malignancies, such as breast cancer, colorectal cancer, and cervical cancer. Li et al [23 ] reported that knockdown of CCAT1 suppresses cell growth and epithelial to mesenchymal transition (EMT) of cervical cancer cells through inhibiting RUNX2 expression. In the study conducted by Jiang et al [24 ], RUNX2 expression was remarkably elevated in recurrent CRC tumours and was intimately related to TMN stages, metastasis, and prognosis of CRC patients.…”

Section: Discussionmentioning

confidence: 99%

Integrated expression analysis revealed RUNX2 upregulation in lung squamous cell carcinoma tissues

Yang

Chen

et al. 2020

IET Systems Biology

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This study aimed to investigate the clinicopathological significance and prospective molecular mechanism of RUNX family transcription factor 2 (RUNX2) in lung squamous cell carcinoma (LUSC). The authors used immunohistochemistry (IHC), RNA-seq, and microarray data from multi-platforms to conduct a comprehensive analysis of the clinicopathological significance and molecular mechanism of RUNX2 in the occurrence and development of LUSC. RUNX2 expression was significantly higher in 16 LUSC tissues than in paired non-cancerous tissues detected by IHC (P < 0.05). RNA-seq data from the combination of TCGA and genotype-tissue expression (GTEx) revealed significantly higher expression of RUNX2 in 502 LUSC samples than in 476 non-cancer samples. The expression of RUNX2 protein was also significantly higher in pathologic T3-T4 than in T1-T2 samples (P = 0.031). The pooled standardised mean difference (SMD) for RUNX2 was 0.87 (95% CI, 0.58-1.16), including 29 microarrays from GEO and one from ArrayExpress. The co-expression network of RUNX2 revealed complicated connections between RUNX2 and 45 co-expressed genes, which were significantly clustered in pathways including ECM-receptor interaction, focal adhesion, protein digestion and absorption, human papillomavirus infection and PI3K-Akt signalling pathway. Overexpression of RUNX2 plays an essential role in the clinical progression of LUSC.

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RETRACTED: Knockdown of long non-coding RNA CCAT1 suppresses proliferation and EMT of human cervical cancer cell lines by down-regulating Runx2

Cited by 13 publications

References 33 publications

RETRACTED: LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway

RETRACTED: LncRNA LIPE-AS1 Predicts Poor Survival of Cervical Cancer and Promotes Its Proliferation and Migration via Modulating miR-195-5p/MAPK Pathway

The Impact of Non-coding RNAs in the Epithelial to Mesenchymal Transition

Integrated expression analysis revealed RUNX2 upregulation in lung squamous cell carcinoma tissues

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