RETRACTED: Long non-coding RNA LINC00467 regulates hepatocellular carcinoma progression by modulating miR-9-5p/PPARA expression

Abstract

The aim of this study was to analyse the expression pattern and elucidate the mechanistic involvement of long non-coding RNA LINC00467 in hepatocellular carcinoma (HCC). The relative expression of LINC00467 and microRNA (miR)-9-5p was determined by real-time polymerase chain reaction. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The cell proliferation was analysed by cell counting. Cell migration and invasion were monitored by Transwell assay. The luciferase reporter assay was employed to investigate the regulatory effect of miR-9-5p on LINC00467 and peroxisome proliferator-activated receptor alpha (PPARA). The endogenous PPARA protein was quantified by western blotting. It was found that LINC00467 was aberrantly decreased in HCC. The ectopic expression of LINC00467 significantly suppressed cell viability, proliferation, migration and invasion. LINC00467 functioned as a sponge for miR-9-5a and negatively regulated miR-9-5p expression. We also identified PPARA as the direct target of miR-9-5p. siRNA-mediated knockdown of PPARA in LINC00467-proficient cells promoted cell viability, migration and invasion. Our data indicate the critical involvement of LINC00467/miR-9-5p/PPARA signalling in the incidence and progression of HCC.

Hepigenetics: A Review of Epigenetic Modulators and Potential Therapies in Hepatocellular Carcinoma

“…LINC00467 , another lncRNA that was found to be downregulated in HCC, has been studied as a potential therapeutic target thanks to its role as an antagomir for miR-9-5a , which targets peroxisome proliferator-activated receptor alpha ( PPARA ) for silencing [ 140 ]. LINC00467 ectopically expressed in HCC cells conduced to antiproliferative effects and, like miR503HG , checked migration and invasion.…”

Overview of distinct 5-methylcytosine profiles of messenger RNA in human hepatocellular carcinoma and paired adjacent non-tumor tissues

“…Hepatocellular carcinoma (HCC) is one of the most widespread cancers, and it has an extremely poor prognosis, contributing to nearly 662,000 deaths per annum [1,2]. The incidence rate of HCC is ranked sixth-highest of the cancerous tumors globally, and the number of deaths caused by HCC is ranked third-highest of the tumor-related deaths [3].…”

Long Noncoding RNA LINC00467 Promotes Glioma Progression through Inhibiting P53 Expression via Binding to DNMT1

“…LINC00467 is a novel lncRNA localized at the chromosomal locus 1q32. 3, and has been reported to be markedly dysregulated in hepatocellular carcinoma [14], lung adenocarcinoma [15] and neuroblastoma [16]. Additionally, it has been confirmed that LIN C00467 could promote cell proliferation, migration and invasion of lung adenocarcinoma cells [17].…”