RETRACTED: Long Noncoding RNA LINC00673 Is Activated by SP1 and Exerts Oncogenic Properties by Interacting with LSD1 and EZH2 in Gastric Cancer

Abstract

Long noncoding RNAs (lncRNAs) have emerged as important regulators in a variety of human diseases, including cancers. However, the biological function of these molecules and the mechanisms responsible for their alteration in gastric cancer (GC) are not fully understood. In this study, we found that lncRNA LINC00673 is significantly upregulated in gastric cancer. Knockdown of LINC00673 inhibited cell proliferation and invasion and induced cell apoptosis, whereas LINC00673 overexpression had the opposite effect. Online transcription factor binding site prediction analysis showed that there are SP1 binding sites in the LINC00673 promoter region. Next, luciferase reporter and chromatin immunoprecipitation (ChIP) assays provided evidence that SP1 could bind directly to the LINC00673 promoter region and activate its transcription. Moreover, mechanistic investigation showed that CADM4, KLF2, and LATS2 might be the underlying targets of LINC00673 in GC cells, and RNA immunoprecipitation, RNA pull-down, and ChIP assays showed that LINC00673 can interact with EZH2 and LSD1, thereby repressing KLF2 and LATS2 expression. Taken together, these findings show that SP1-activated LINC00673 exerts an oncogenic function that promotes GC development and progression, at least in part, by functioning as a scaffold for LSD1 and EZH2 and repressing KLF2 and LATS2 expression.

A Novel LncRNA-miRNA-mRNA Triple Network Identifies LncRNA RP11-363E7.4 as An Important Regulator of miRNA and Gene Expression in Gastric Cancer

“…In this study, by retrieving relevant previous reports through the keywords "gastric cancer and RNA," we finally selected four cancer-related protein coding genes, which have been tested by different kinds of experiments. These four genes ("NOV," "KIT," "SP1," and "CLOCK") were considered as seeds in the RWR model [33][34][35][36]. The initial probability P 0 of each seed mRNA was set as 1/n (where n is the number of seed mRNAs), while other non-seed mRNAs were set as 0.…”

Long Non-Coding RNA SH3PXD2A-AS1 Promotes Cell Progression Partly Through Epigenetic Silencing P57 and KLF2 in Colorectal Cancer

“…Importantly, progress in our understanding of CRC can be accelerated by elucidating the regulatory mechanisms involved in CRC carcinogenesis and development. Current evidence has highlighted the roles of long non-coding RNAs (lncRNAs) as crucial modulators in multiple types of tumors, including CRC [4-7]. Thus, novel insights into the involvement of lncRNAs are urgently needed.…”

KDM1A regulated the osteo/dentinogenic differentiation process of the stem cells of the apical papilla via binding with PLOD2

“…We then investigated the regulatory mechanism of KDM1A in SCAPs. KDM1A has been found to form protein complexes with other proteins during cell proliferation and differentiation . In the present study, we identified several candidate binding partners of KDM1A including PLOD2.…”