Background: The present study investigated the protective effects of carvacrol (CAR) against testicular toxicity induced by sodium arsenite (SA).Methods and Results: Rats were given SA and/or CAR for 14 days. Semen analyzes showed that CAR increased sperm motility and decreased the percentage of anarmol and dead sperm. It was determined that the oxidative stress induced by SA decreased with the increase of Nrf-2 and HO-1 expressions, SOD, CAT, GPx and GSH levels, and MDA levels decreased after CAR treatment. It was observed that autophagy and in ammation triggered by SA in testicular tissue were alleviated by suppressing the expressions of LC3A, LC3B, MAPK-14, NF-kB, TNF-a, IL-1b, iNOS and COX-2 biomarkers in rats given CAR. CAR treatment protected against apoptosis by suppressing Bax and Caspase-3 expressions and triggering Bcl-2 expression in testicles.Conclusions: As a result, it was seen that CAR could provide protection against SA-induced damage in testicular tissues of rats.