Retromer subunit, CfVps35 is required for growth development and pathogenicity of Colletotrichum fructicola

Li, Xi-Ya; Zhang, Sheng-Pei; He, Li

doi:10.1186/s12863-022-01084-4

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…During pathogen-host interactions, pathogens exploit the endolysosomal network to fine-tune intracellular homeostasis, thereby facilitating host colonization 9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30 . However, the complex interplay within the endolysosomal network and its potential in fungal disease control remain largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Highlighting its importance, the endolysosomal network is associated with the pathogenesis of plant pathogenic fungi. Dysfunction of the endolysosomal network impairs the vegetative growth and infection-related development of various fungal pathogens 9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30 . Additionally, the endolysosomal network is required for effective modulation of autophagy process, which helps in adaptation to metabolic changes and environmental cues during pathogen-host interactions 21,26,27,28,29 .…”

Section: Introductionmentioning

confidence: 99%

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Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

Chen,

Long

et al. 2024

Preprint

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During host-pathogen interactions, pathogens employ endolysosomal trafficking to fine-tune host and environmental cues, facilitating disease progression. Inhibiting the functions of the endolysosomal network could be promising for the control of plant fungal diseases. Herein, we showed that inhibition of the endocytic dynamin FgVps1 by the phenothiazines chlorpromazine (CPZ) and prochlorperazine (PCZ) leads to endolysosomal dysfunction. Further analyses revealed that the phenothiazines directly bind the GTPase domain of FgVps1 to inhibit its activity. Moreover, our results showed that FgVps1 is recruited to the endosome by the Rab GTPases FgRab51 and FgRab7 to facilitate the release of retromer- and sorting nexin-coated vesicles, thereby ensuring the proper recycling of some cargos and promoting fungal development and pathogenicity. Direct treatment of the phytopathogensF. graminearum,Magnaporthe oryzaeandFusarium oxysporum f. sp. cubensewith CPZ and PCZ also impairs their growth, development and virulence. These results unveil the underlying mechanisms of endocytic dynamin actions and provide a potential strategy for broad-spectrum control of phytopathogenic fungi.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

Chen,

Long

et al. 2024

Preprint

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The endosomal–vacuolar transport system acts as a docking platform for the Pmk1 MAP kinase signaling pathway in Magnaporthe oryzae

Wang,

Huang

et al. 2024

New Phytologist

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Summary In Magnaporthe oryzae, the Pmk1 MAP kinase signaling pathway regulates appressorium formation, plant penetration, effector secretion, and invasive growth. While the Mst11‐Mst7‐Pmk1 cascade was characterized two decades ago, knowledge of its signaling in the intracellular network remains limited. In this study, we demonstrate that the endosomal surface scaffolds Pmk1 MAPK signaling and Msb2 activates Ras2 on endosomes in M. oryzae. Protein colocalization demonstrated that Msb2, Ras2, Cap1, Mst50, Mst11, Mst7, and Pmk1 attach to late endosomal membranes. Damage to the endosome–vacuole transport system influences Pmk1 phosphorylation. When Msb2 senses a plant signal, it internalizes and activates Ras2 on endosome membrane surfaces, transmitting the signal to Pmk1 via Mst11 and Mst7. Signal‐sensing and delivery proteins are ubiquitinated and sorted for degradation in late endosomes and vacuoles, terminating signaling. Plant penetration and lowered intracellular turgor are required for the transition from late endosomes to vacuoles in appressoria. Our findings uncover an effective mechanism that scaffolds and controls Pmk1 MAPK signaling through endosomal–vacuolar transport, offering new knowledge for the cytological and molecular mechanisms by which the Pmk1 MAPK pathway modulates development and pathogenicity in M. oryzae.

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Protein Disulfide Isomerase CfPdi1 Is Required for Response to ER Stress, Autophagy, and Pathogenicity in Colletotrichum fructicola

Chen²,

2023

Forests

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Camellia oleifera, a woody plant indigenous to China, is primarily utilized for the production of cooking oil. However, it is frequently afflicted by anthracnose, a highly detrimental disease that leads to significant annual losses. Colletotrichum fructicola is the predominant etiological agent responsible for anthracnose in Ca. oleifera. Additionally, our investigation has revealed that a bZIP transcription factor CfHac1 in C. fructicola governs the pathogenicity and response to endoplasmic reticulum stress. In this study, we conducted an investigation of the role of the CfPDI1 gene in C. fructicola, which was significantly downregulated in ΔCfhac1 under endoplasmic reticulum stress. The CfPDI1 gene was deleted, resulting in reduced vegetative growth, conidiation, appressoria formation, and appressorium turgor generation. Furthermore, it was observed that the ΔCfpdi1 mutant exhibited impaired responsiveness to endoplasmic reticulum stresses, and the expression of UPR-related genes in C. fructicola was influenced by CfPdi1. Cytological investigations indicated that CfPdi1 is localized in the endoplasmic reticulum. Further analysis revealed that the ΔCfpdi1 mutant displays significantly reduced pathogenicity in Ca. oleifera. Taken together, this study illustrated crucial functions of CfPdi1 in development, response to ER stress, autophagy, and pathogenicity in C. fructicola.

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Retromer subunit, CfVps35 is required for growth development and pathogenicity of Colletotrichum fructicola

Cited by 4 publications

References 35 publications

Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

The endosomal–vacuolar transport system acts as a docking platform for the Pmk1 MAP kinase signaling pathway in Magnaporthe oryzae

Protein Disulfide Isomerase CfPdi1 Is Required for Response to ER Stress, Autophagy, and Pathogenicity in Colletotrichum fructicola

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