Retrospective detection and phylogenetic analysis of pseudorabies virus in dogs in China

Tu, Lu; Lian, Jiamin; Pang, Yanling; Liu, Cun; Cui, Shangjin; Lin, Wencheng

doi:10.1007/s00705-020-04848-4

Cited by 9 publications

(13 citation statements)

References 31 publications

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“…While the noti cation of infection in domestic pigs decreases in some regions of the world, PrV reported in dogs from different parts of the world is increasing [9,10,13,15,16,24]. The genomic characterization of PrV from secondary host as obtained in this study will help to trace the population diversity and infection origin for the areas where the disease has not yet been detected or is still a problem.…”

Section: Discussionmentioning

confidence: 84%

First isolation and molecular characterization of pseudorabies virus detected in Turkey

et al. 2022

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Pigs are the main host species for the pseudorabies virus, while it causes fatal encephalitis in many species including human being. The objective of this article is to report the rst clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. The dog showing clinical signs including pruritus and neurological signs as stumbling and inability to stand up compatible with pseudorabies. The virus isolate was obtained from supernatant of fresh tissue samples from cerebellum, cornu ammonis, spleen, salivary gland, and conjunctival swab, as well as serum, and buffy coat samples. The glycoprotein C region is targeted for viral DNA ampli cation. Pseudorabies virus genome was detected both in fresh tissues and supernatants of 3 rd passage on Vero cells. Number of PCR positive samples were dramatically increased after having cell culture inoculations. Genome sequencing revealed that the strain (Bursa-10303) isolated in Turkey where a non-endemic area is for the virus is dropped into clade A. This study con rms the presence of psedorabies infection in the wild life reservoirs in Turkey. Future studies may clarify the importance of the infection for Turkey where there is no common pig production.

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Section: Discussionmentioning

confidence: 84%

First isolation and molecular characterization of pseudorabies virus detected in Turkey

et al. 2022

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“…The pathogenic PRV strain SD18 (Batch No. MN443976), a variant PRV strain circulating in the Chinese swine industry, was isolated in the Shandong province of China in 2018 ( 14 ). In total, nine 10-week-old domestic cats were purchased from the Guangdong Research Center of Laboratory Animals (approval ID: SCXK-2013-0007).…”

Section: Methodsmentioning

confidence: 99%

“…The presence of PRV was detected using real-time quantitative PCR assay as previously described ( 14 ). Viral DNA was extracted from clinical samples using TRIzol reagent (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

“…Viral DNA was extracted from clinical samples using TRIzol reagent (Invitrogen). The specific primer pairs 5-TGAAGCGGTTCGTGATGG-3 and 5-CCCCGCACAAGTTCAAGG-3 targeting the PRV gB gene and the specific primer pairs 5-CCGCGGGCCGTGTTCTTTGT-3 and 5-GCGCCGGCGAGGTGAAGC-3 targeting the PRV gE gene were designed according to previous studies ( 14 ). Real-time quantitative PCR was performed as previously described ( 14 ).…”

Section: Methodsmentioning

confidence: 99%

“…The specific primer pairs 5-TGAAGCGGTTCGTGATGG-3 and 5-CCCCGCACAAGTTCAAGG-3 targeting the PRV gB gene and the specific primer pairs 5-CCGCGGGCCGTGTTCTTTGT-3 and 5-GCGCCGGCGAGGTGAAGC-3 targeting the PRV gE gene were designed according to previous studies ( 14 ). Real-time quantitative PCR was performed as previously described ( 14 ). Briefly, the recipe contains 10 μl of a 2 × SYBR Premix ExTaq Green mix (TaKaRa), 1 μl of a template, and 0.5-mm concentration of specific primers.…”

Section: Methodsmentioning

confidence: 99%

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Assessing the Risk of Commercial Vaccines Against Pseudorabies Virus in Cats

Zhao

Chen

et al. 2022

Front. Vet. Sci.

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Pseudorabies virus (PRV) is a zoonotic agent that causes significant economic losses in animal husbandry worldwide, and gE-deleted vaccines play an important role in its treatment in the swine industry. However, the potential risk of attenuated PRV strains in commercial vaccines for other hosts remains unclear. Especially, cats are important companion animals for human beings. In this study, we investigated the prevalence and pathogenicity of the PRV wild strain in the cat population. We found that the occurrence of PR diseases in cats is sporadic, that the attenuated PRV strain causes slight clinical signs in cats, and that the virus is excreted 3 days post-infection. Our findings will be beneficial in furthering our understanding of the epidemiology and pathogenicity of PRV in cats and implying the great risk of RPV transmission from pigs to cats.

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Pseudorabies

Henke¹,

Vandevelde²

2021

Greene's Infectious Diseases of the Dog and Cat

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Retrospective detection and phylogenetic analysis of pseudorabies virus in dogs in China

Cited by 9 publications

References 31 publications

First isolation and molecular characterization of pseudorabies virus detected in Turkey

First isolation and molecular characterization of pseudorabies virus detected in Turkey

Assessing the Risk of Commercial Vaccines Against Pseudorabies Virus in Cats

Pseudorabies

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