Reusable electrochemical biosensing platform based on egg yolk antibody-labeled magnetic covalent organic framework for on-site detection of Escherichia coli in foods

Xiao, Shu; Yang, Xin; Wu, Jiayi; Liu, Qili; Li, Dengfeng; Huang, Shenfeng; Xie, Hongzhen; Yu, Zhenzhong; Gan, Ning

doi:10.1016/j.snb.2022.132320

Cited by 30 publications

(10 citation statements)

References 47 publications

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“…However, the cost of using cTnl as the target is expensive at present. Gan et al 135 selected to use egg yolk antibody (IgY) to purify chicken antibodies with specific analytes. A magnetic COF microsphere with a large specific surface area and high porosity was prepared, which was further loaded onto the surface of Fe 3 O 4 microspheres.…”

Section: Biosensing and Bioimagingmentioning

confidence: 99%

Recent bio-applications of covalent organic framework-based nanomaterials

Guo,

Kong,

Lian

et al. 2024

Chem. Commun.

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Section: Biosensing and Bioimagingmentioning

confidence: 99%

Recent bio-applications of covalent organic framework-based nanomaterials

Guo,

Kong,

Lian

et al. 2024

Chem. Commun.

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“…Although these methods have high accuracy and speed, the high cost and need for specialized knowledge and complex equipment are the disadvantages of these methods [13]. Several types of biosensors such as aptasensor [14], immunosensor [15], carbohydrate biosensor [16], and enzymatic biosensor [17] have been developed to identify pathogens including E. coli bacteria using different techniques such as electrochemical [18], colorimetric [19], optical [20], and quartz crystal microbalance (QCM) methods [21].…”

Section: Introductionmentioning

confidence: 99%

First Label-Free Carbohydrate-Based Electrochemical Sensor To Detect Escherichia coli Pathogenic Bacteria Using Affinity Between Adhesion FimH and Mannose on the Glassy Carbon Electrode

Zadeha¹,

Kashanian²,

Nazari³

2023

Preprint

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Controlling water and food contamination by pathogenic organisms requires quick, simple, and low-cost methods. using affinity between mannose and type I fimbriae in the cell wall of Escherichia coli bacteria as evaluation elements compared to the conventional plate counting technique enables a reliable sensing platform for the detection of bacteria. In this study, a simple new sensor based on electrochemical impedance spectroscopy (EIS) for rapid and sensitive detection of Escherichia coli was developed. Biorecognition layer of the sensor was formed by covalent attachment of p-carboxyphenylamino mannose (PCAM) to gold nanoparticles (AuNPs) electrodeposited on the surface of glassy carbon electrode (GCE). The resultant structure of PCAM was characterized and confirmed using FTIR. The developed biosensor demonstrated a linear response with a logarithm of bacterial concentration (R2 = 0.998) in the range of 1.3 × 10 1~ 1.3 × 106 CFU.mL−1with the limit of detection of 2 CFU.mL−1 within 60 min. The sensor did not generate any significant signals with two non-target strains, demonstrating high selectivity of the developed biorecognition chemistry. Selectivity of the sensor and its applicability to analysis of the real samples was investigated in samples of tap water. Overall, the developed sensor has shown to be promising for the detection of E. coli pathogen in water due to its high sensitivity, short detection time, low cost, high specificity, and user-friendliness.

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“…Electrochemical immunosensors are characterized by short detection time [10], simple operation, high sensitivity [11], miniaturization, and low cost [12]. Recently, electrochemical immunosensors have been developed for E. coli O157:H7 detection [13][14][15][16]. Mo et al [17] constructed a sandwich electrochemical immunosensor for E. coli O157:H7 with good speci city.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

Qian,

Lu,

Zheng

et al. 2023

Preprint

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In this study, a sandwich immunosensor based on the conjugate of gold nanoparticles and quantum dot microspheres on polypyrrole/polyaniline (PPY-PANI) composite membrane was developed for the detection of Escherichia coli O157:H7 (E. coli O157:H7). Quantum dot microsphere conjugates (QBC), as immunoprobes throughout electrochemical detection, exhibited signal amplifications. Each stage of the immunosensor preparation was characterized by cyclic voltammetry, and electrochemical impedance spectroscopy. The results showed that the optimal reaction conditions of antigen and secondary antibody were 37 ℃ and 60 min respectively, and the concentration of secondary antibody was 0.01 mg·mL− 1. When E. coli O157:H7 was performed in the immunosensor of PPY-PANI-Ab2, E. coli O157:H7 in the concentration range of 4.73×102-4.73×109 CFU·mL− 1, exhibited a linear relationship between the change of current (ΔIpa) and the logarithm of bacterial concentration. Compared to 100 CFU·mL− 1 of the limit of detection (LOD) in normal saline solution, the lowest detection limit in 0.1% peptone water was 113 CFU·mL− 1, and the signal-to-noise ratio was 3. Moreover, the recovery rate of spiked milk and shrimp were 80.80-98.36% and 75.20-94.31%, and the relative standard deviation (RSD) was 3.03–6.18% and 1.61–6.94% respectively. Therefore, the immunosensor of with low cost and good stability is expected to be used for rapid screening of E. coli O157:H7 in food.

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Reusable electrochemical biosensing platform based on egg yolk antibody-labeled magnetic covalent organic framework for on-site detection of Escherichia coli in foods

Cited by 30 publications

References 47 publications

Recent bio-applications of covalent organic framework-based nanomaterials

Recent bio-applications of covalent organic framework-based nanomaterials

First Label-Free Carbohydrate-Based Electrochemical Sensor To Detect Escherichia coli Pathogenic Bacteria Using Affinity Between Adhesion FimH and Mannose on the Glassy Carbon Electrode

Electrochemical immunosensor of E. coli O157:H7 in food based on PPY-PANI and quantum dot microsphere conjugates

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