Reusable, facile, and rapid aptasensor capable of online determination of trace mercury

Chen, Yangyang; Zhu, Qian; Zhou, Xin; Wang, Ruoyu; Yang, Zhiqing

doi:10.1016/j.envint.2020.106181

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…Compared with other targets, the simple structure and single binding site for heavy metal ions increase the difficulty for aptamer recognition [ 74 ]. The combination between heavy metal ions and aptamers will not occur with obvious mass change and steric hindrance effect [ 75 ].…”

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

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Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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“…Moreover, as the binding of the aptamers to their targets is reversible, aptamer-based sensors can be regenerated and reused multiple times, leading to more cost-effective sensors [ 91 ]. The regeneration has been demonstrated by washing with a running buffer [ 92 ] or guanidine hydrochloride solution [ 93 ], incubation in 10% ( w / v ) sodium dodecyl sulfate [ 94 ], and introducing azobenzene moiety into the aptamer chain [ 95 ].…”

Section: Biorecognition Requirements—nucleic Acid-based Assaysmentioning

confidence: 99%

Recent Advances, Opportunities, and Challenges in Developing Nucleic Acid Integrated Wearable Biosensors for Expanding the Capabilities of Wearable Technologies in Health Monitoring

et al. 2022

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Wearable biosensors are becoming increasingly popular due to the rise in demand for non-invasive, real-time monitoring of health and personalized medicine. Traditionally, wearable biosensors have explored protein-based enzymatic and affinity-based detection strategies. However, in the past decade, with the success of nucleic acid-based point-of-care diagnostics, a paradigm shift has been observed in integrating nucleic acid-based assays into wearable sensors, offering better stability, enhanced analytical performance, and better clinical applicability. This narrative review builds upon the current state and advances in utilizing nucleic acid-based assays, including oligonucleotides, nucleic acid, aptamers, and CRISPR-Cas, in wearable biosensing. The review also discusses the three fundamental blocks, i.e., fabrication requirements, biomolecule integration, and transduction mechanism, for creating nucleic acid integrated wearable biosensors.

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“…Real-time optical mercury(II) determination can be carried out using an aptasensor constructed on a waveguide platform, as presented by Chen et al [ 65 ]. The sensor was based on the competition of the interaction of a fluorescently labeled aptamer (Cy5.5-ATCCCCTTTGTTTGTTTAGCCCCTATTCTTTCTTGGTCTGC) with mercury and a complementary sequence on the surface (GCAGACCAAGAAA).…”

Section: Aptasensors For Metal Ions Detectionmentioning

confidence: 99%

Recent Achievements in Electrochemical and Optical Nucleic Acids Based Detection of Metal Ions

Jarczewska

Szymczyk²,

Zajda³

et al. 2022

Molecules

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Recently nucleic acids gained considerable attention as selective receptors of metal ions. This is because of the possibility of adjusting their sequences in new aptamers selection, as well as the convenience of elaborating new detection mechanisms. Such a flexibility allows for easy utilization of newly emerging nanomaterials for the development of detection devices. This, in turn, can significantly increase, e.g., analytical signal intensity, both optical and electrochemical, and the same can allow for obtaining exceptionally low detection limits and fast biosensor responses. All these properties, together with low power consumption, make nucleic acids biosensors perfect candidates as detection elements of fully automatic portable microfluidic devices. This review provides current progress in nucleic acids application in monitoring environmentally and clinically important metal ions in the electrochemical or optical manner. In addition, several examples of such biosensor applications in portable microfluidic devices are shown.

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Reusable, facile, and rapid aptasensor capable of online determination of trace mercury

Cited by 8 publications

References 35 publications

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Recent Advances, Opportunities, and Challenges in Developing Nucleic Acid Integrated Wearable Biosensors for Expanding the Capabilities of Wearable Technologies in Health Monitoring

Recent Achievements in Electrochemical and Optical Nucleic Acids Based Detection of Metal Ions

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