Reusable fiber optic immunosensor for rapid detection of imazethapyr herbicide

Anis, Nabil A.; Eldefrawi, Mohyee E.; Wong, Rosie B.

doi:10.1021/jf00029a031

Cited by 43 publications

(21 citation statements)

References 10 publications

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“…Finally, we anticipate that the optimized displacement method (coupled with QCM) and model for TNT detection can be modified for the detection of other molecules of interest (e.g. imazethapyr [49] or cocaine [50]), provided that an antibody specific for the molecule of interest, and displaying cross reactivity for analogous molecules that typically limit its detection, has been isolated or engineered.…”

Section: Discussionmentioning

confidence: 99%

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Wang

AlMakhaita²,

Biswal³

et al. 2012

J Biosens Bioelectron

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Currently available TNT sensors are characterized by high sensitivity, but low specificity, which limits the detection of TNT in dirty environments. We report here a TNT sensor designed to measure the displacement of a TNT-specific antibody by quartz crystal microbalance (QCM). This sensor combines high sensitivity of detection (0.1 ng/mL) with the ability to distinguish TNT from molecules with similar chemical properties. Particularly, the reliability of this method for the detection of TNT in dirty environments was investigated by using fertilizer solution and artificial seawater. Instead of measuring actual binding of TNT, the method described is based on the displacement of an anti-TNT antibody, which allows quantifying the concentration of TNT in solution with higher sensitivity. In addition, by utilizing the rate of antibody displacement, the detection time is significantly decreased from hours, which would be necessary to measure the frequency change at equilibrium, to minutes. A Langmuir kinetic model was used to describe the molecular interactions on the surface of the sensor and to establish a standard curve to estimate on-site TNT detection. In summary, QCM detection of anti-TNT antibody displacement provides a method for rapid detection of TNT with high sensitivity and specificity.

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Section: Discussionmentioning

confidence: 99%

Sensitive Detection of TNT using Competition Assay on Quartz Crystal Microbalance

Wang

AlMakhaita²,

Biswal³

et al. 2012

J Biosens Bioelectron

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show abstract

“…The observed bulk concentration limit of detection for the biotin/streptavidin bioassay is 15 nM, which is on the order of the LOD reported for several de-clad fiber fluorescence sensors. (Anis et al, 1993;Devine et al, 1995;Eenink et al, 1990;Graham et al, 1992;McCormack et al, 1997;Oroszlan et al, 1993;Pandey & Weetall, 1995;Shriver-Lake et al, 1995;Sutherland et al, 1984) However, the measured pathlength of the FOC is only ~ 24 mm for this device compared to the ~ 60 mm pathlength of most cylindrical core de-clad fiber sensor platforms. The de-clad fiber sensor platforms require large fiber cores (~ 600 µm) with low modal surface interaction to increase mechanical strength, and therefore, a long interaction length is necessary.…”

Section: Bsa-biotin/streptavidin-cy Bioassay On the Planar Fiber Optimentioning

confidence: 95%

“…The pioneering research utilizing a de-clad quartz fiber to collect back-coupled fluorescence from immobilized biomolecules was presented by Sutherland et.al, Andrade et.al, and Glass et.al. Biosensors based on receptor proteins (Garden et al, 2004;Rogers et al, 1991;Rogers et al, 1989), antibody-antigen interactions (Anis et al, 1993;Bier et al, 1992;Devine et al, 1995;Eenink et al, 1990;McCormack et al, 1997;Oroszlan et al, 1993;Shriver-Lake et al, 1995;Toppozada et al, 1997;Walczak et al, 1992), sandwich immunoassay (Geng et al, 2006;Kapoor et al, 2004), and oligonucleotides (Abel et al, 1996;Graham et al, 1992;Pandey & Weetall, 1995) have been presented employing the de-clad fiber geometry. However, the declad fiber architecture is limited by the fragile nature of the fiber platform and inefficient fluorescence back-coupling due to the sharp V-number mismatch.…”

Section: Fiber Optic Based Fluorescence Sensorsmentioning

confidence: 99%

Applications of the Planar Fiber Optic Chip

Beam¹,

Burnett²,

Webster³

et al. 2012

Recent Progress in Optical Fiber Research

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“…Enzymatic labels used in immunosensor devices are usually ox i d o reductases such as peroxidase (HRP) or hy d ro ly t i c enzymes such as alkaline phosphatase. Immunosensors can be divided into four classes, depending on the transducer e m p l oye d : p i e zo e l e c t ric [12][13][14], optical [15][16][17], e l e c t rochemical [18,19], or thermometric. The immobilized sensing element can be either an antibody (AB) or an antigen (AG) wich can be chemically modified (hapten).…”

Section: Immunosensorsmentioning

confidence: 99%