Revealing holistic metabolic responses associated with lipid and docosahexaenoic acid (DHA) production in Aurantiochytrium sp. SW1

Prabhakaran, Pranesha; Raethong, Nachon; Thananusak, Roypim; Nazir, Mohamed Yusuf Mohamed; Sapkaew, Chakkapan; Soommat, Panyawarin; Kingkaw, Amornthep; Hamid, Aidil Abdul; Vongsangnak, Wanwipa; Song, Yuanda

doi:10.1016/j.bbalip.2023.159306

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…Previous studies have demonstrated that cell growth and lipid accumulation in Aurantiochytrium are not synchronized. The synthesis of DHA-rich oils often begins in response to external stress conditions, such as the depletion of nitrogen sources [30,39]. Therefore, promoting the growth of highly vital cells in the early stage is beneficial for adapting to adverse environments and facilitating lipid accumulation in the later stage [40].…”

Section: Aurantiochytrium Cellular Residues As a Nitrogen Source For ...mentioning

confidence: 99%

Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids

Yin,

Huang,

Zhan

et al. 2024

Fermentation

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Cellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the utilization of enzymatically hydrolyzed Aurantiochytrium cellular residues (ACRs) and Mortierella alpina cellular residues (MCRs) as nitrogen sources. Results demonstrated that ACRs and MCRs could partially substitute yeast extract (YE) without adverse effects on DHA and ARA fermentation. Moreover, the implementation of a new fermentation medium incorporating ACRs, MCRs, and YE as mixed nitrogen sources resulted in DHA and ARA yields of 17.78 and 5.77 g/L, respectively. These values represented increases of 10.37% and 9.28% compared to traditional cultural methods, while simultaneously reducing the usage of YE by 80% and 60%, respectively. Therefore, the strategy of recycling waste cellular residues presents a novel approach for reducing the costs and environmental impact associated with microbial fermentations.

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Section: Aurantiochytrium Cellular Residues As a Nitrogen Source For ...mentioning

confidence: 99%

Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids

Yin,

Huang,

Zhan

et al. 2024

Fermentation

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Overview of Docosahexaenoic Acid (DHA) Biosynthesis by Aurantiochytrium : Biosynthetic Pathway, High-Yield Mechanism, and Metabolic Engineering Strategies

Zhang,

Zhao,

Zhang

et al. 2024

Food Reviews International

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Comparative analysis of process intensification technologies (PIT) for improved cell disruption and lipid recovery in Aurantiochytrium sp. SW1 microalgae

Mokhtar,

Abdul Rahman,

Sofian‐Seng

et al. 2024

Int J of Food Sci Tech

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SummaryThis study explores enhancing lipid recovery from the lipid‐rich marine microalgae Aurantiochytrium sp. SW1, known for its robust cell wall posing challenges, which necessitates energy‐intensive cell‐disruption for efficient intracellular lipid extraction. Despite advancements in other microalgae, there is lack of reports on efficient cell disruption for Aurantiochytrium sp. SW1 which is vital to reduce the energy costs and minimising the downstream process while maximising lipid yields. Three process intensification technologies (PIT) – microwave treatment, autoclave treatment and ultrasonication were employed on wet Aurantiochytrium sp. SW1 cells to improve the lipid extraction efficiency. Assessing the disruption effectiveness via lipid content, suspension turbidity and particle size distribution, alongside scanning electron microscopy (SEM) for cellular morphology changes post‐PIT treatments, this study indicates all three methods reduced particle size with prolonged treatment time, implying successful cell wall disruption and intracellular component release. Ultrasonication, especially at 50 W power for 10 min, exhibited the highest efficacy, yielding lipid content of 67.76 ± 0.03%. Microscopic analysis unveiled ultrasonication‐induced cell shrinkage and increased membrane permeability, supporting its effectiveness in enhancing solvent penetration for improved lipid recovery. This research underscores PIT techniques' potential as sustainable, energy‐efficient strategies for lipid extraction from Aurantiochytrium sp. SW1 microalgae.

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Revealing holistic metabolic responses associated with lipid and docosahexaenoic acid (DHA) production in Aurantiochytrium sp. SW1

Cited by 3 publications

References 69 publications

Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids

Recycling Fermentation Strategy for Waste Cellular Residues in the Production of Polyunsaturated Fatty Acids

Overview of Docosahexaenoic Acid (DHA) Biosynthesis by Aurantiochytrium : Biosynthetic Pathway, High-Yield Mechanism, and Metabolic Engineering Strategies

Comparative analysis of process intensification technologies (PIT) for improved cell disruption and lipid recovery in Aurantiochytrium sp. SW1 microalgae

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