Reverse hydrolytic process for O-alkylation of glucose catalysed by immobilized α- and β-glucosidases

Kosary, J.; Stefanovits-Bányai, Éva; Boross, L.

doi:10.1016/s0168-1656(98)00160-6

Cited by 25 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This peculiar nature of our new isolate could be explored as a natural immobilized enzyme system in various applications wherein immobilization of these enzymes is needed. Through conventional method of immobilization, α-and β-glucosidase have been used by various researchers in Oalkylation of glucose (Kosary et al 1998), hydrolysis of cellulose to glucose (Venardos et al 1980), flavor enhancement in wine making processes (Palmeri and Spagna 2007), and flavor and sensory properties enhancement of fruit juices (Gallifuoco et al 1998;Riou et al 1998). However, these conventional processes of immobilization are poor in cost effectiveness apart from technical draw backs such as leakage of enzymes from the matrix and time consuming methodologies.…”

Section: Solubilization Of Cell-bound Enzymementioning

confidence: 99%

Production of Cell Membrane-Bound α- and β-Glucosidase by Lactobacillus acidophilus

Mahajan

Desai

Lele

2010

Food Bioprocess Technol

View full text Add to dashboard Cite

Hydrolytic enzymes, viz. α-and β-glucosidase, were produced from indigenous isolate, Lactobacillus acidophilus, isolated from fermented Eleusine coracana. Production of these enzymes was enhanced by optimizing media using one factor at a time followed by response surface methodology. The optimized media resulted in a 2.5-and 2.1-fold increase in α-and β-glucosidase production compared with their production in basal MRS medium. Localization studies indicated 80% of the total activity to be present in the cell membrane-bound fraction. Lack of sufficient release of these enzymes using various physical, chemical, and enzymatic methods confirmed their unique characteristic of being tightly cell membrane bound. Enzyme characterization revealed that both α-and β-glucosidase exhibited optimum catalytic activity at 50°C and pH 6.0 and 5.0, respectively. K m and V max of α-glucosidase were 4.31 mM and 149 μmolmin −1 mL −1 for pnitrophenyl-α-D-glucopyranoside as substrate and 3.8 mM and 120 μmolmin −1 mL −1 for β-glucosidase using p-nitrophenyl-β-D-glucopyranoside as the substrate.

show abstract

Section: Solubilization Of Cell-bound Enzymementioning

confidence: 99%

Production of Cell Membrane-Bound α- and β-Glucosidase by Lactobacillus acidophilus

Mahajan

Desai

Lele

2010

Food Bioprocess Technol

View full text Add to dashboard Cite

show abstract

“…The diff erences in solubility of the two substrates are the reason why for a very long time they were synthesised exclusively in two-phase systems with both free and immobilised β-glycosidases, and via both reverse hydrolysis and transglycosylation reactions. In the water-saturated reaction systems for alkyl-glycoside production, hydrolysis of the already formed alkyl-β-glucoside, the so-called secondary hydrolysis, was responsible for low product yields (12)(13)(14)(15)(16)(17)(18). While the reverse hydrolysis, or the so--called condensation reaction (19,20) is thermodynamically controlled and is a direct reversal of hydrolysis, the glycosyl transfer reaction, called also transglycosylation, is kinetically controlled.…”

Section: Synthesis Of Octyl-β-glucoside Catalyzed By Almond β-Glucosimentioning

confidence: 99%

Synthesis of Octyl-β-Glucoside Catalyzed by Almond β-Glucosidase in Unconventional Reaction Media

Mladenoska

2016

Food Technol. Biotechnol.

View full text Add to dashboard Cite

SummaryThe reaction system for octyl-β-glucoside synthesis catalysed by the almond-β-glu cosidase has been characterised. The monophasic octanol saturated with diff erent amounts of water served as a reaction medium. Both the glucose and the activated substrate p-nitrophenyl-β-glucoside were examined as glycon donors. The reverse hydrolysis and the transglycosylation were both used as reaction models for this enzymatically catalysed alkyl-β-glucoside synthesis. The rate of synthesis of octyl-β-glucoside (v S ), the rate of hydrolysis, i.e. the glucose formation (v H ) and the predicted yield (Y) were determined. The eff ect of water activity on the synthetic and hydrolytic activity of the enzyme was investigated. Both the rate of synthesis and the rate of hydrolysis increased with the increase of the water activity in the reaction system, showing their maximum values at the water activity close to the saturation level. Thus, the maximum ratio of v S /v H =0.165 was achieved at the water activity of 0.94. The predicted yields were 0.5, 0.75 and 14.19 % and were lower than the actually achieved yields of 19.45, 38 and 36.40 % at the water activities of 0.75, 0.84 and 0.94, respectively. The yield of octyl-β-glucoside in the reverse hydrolysis was only 15.2 %, i.e. 3.25 times lower than the yield obtained in the transglycosylation reaction with the water activity of 0.94. The solubility of glucose in pure octanol was only 1.5 mmol/L at the saturation level of 12 mmol/L in the presence of 10 mmol/L of p-nitrophenyl-β-glu coside, and it increased to 15.5 mmol/L in the presence of octyl-β-glucoside.

show abstract

“…33 The low yields obtained with the native glucosidases were overcome by immobilization of the enzymes on a modified polyacrylamide-type bead support (Acrylex C-100). This increased enzyme stability and resulted in higher yields.…”

Section: Yeast and Fungal Glucosidasesmentioning

confidence: 99%

Production of Surface Active Compounds by Biocatalyst Technology

Zinjarde

Ghosh

2010

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

In the current scenario, there is immense concern regarding the environmental issues. Eco-friendly surfactants are becoming a preferred choice for specific applications in spite of their possibly inferior performance or more expensive nature than conventional ones. This chapter deals with the use of enzymes in non-aqueous media for the synthesis of surfactants such as monoglycerides, sugar fatty acid esters, fatty acid amides, alkyl glycosides and lysophospholipids. The conventional methods of synthesizing these classes of surfactants, the variety of enzymes involved, process parameters, yields, advantages and disadvantages have been discussed in detail.

show abstract

Reverse hydrolytic process for O-alkylation of glucose catalysed by immobilized α- and β-glucosidases

Cited by 25 publications

References 11 publications

Production of Cell Membrane-Bound α- and β-Glucosidase by Lactobacillus acidophilus

Production of Cell Membrane-Bound α- and β-Glucosidase by Lactobacillus acidophilus

Synthesis of Octyl-β-Glucoside Catalyzed by Almond β-Glucosidase in Unconventional Reaction Media

Production of Surface Active Compounds by Biocatalyst Technology

Contact Info

Product

Resources

About