Reverse Micellar Extraction of β-Galactosidase from Barley (Hordeum vulgare)

Hemavathi, A. B.; Hebbar, H. Umesh; Raghavarao, K.S.M.S.

doi:10.1007/s12010-008-8228-x

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…Several reports on the application of RME for separation and purification of biomolecules are available for model systems (4)(5)(6)(7)(8). In recent years, a few studies on RME of biomolecules from natural=real systems have been reported (9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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Scale-up studies for phase transfer mode of reverse micellar extraction are attempted for the separation and primary purification of bromelain (EC 3.4.22.33) from pineapple (Ananas comosus L. Merryl) waste. Characterization of reverse micelles and mass transfer studies for the real system has been attempted for the first time. Scale-up of the extraction process employing commercial grade surfactant cetyltrimethylammonium bromide (CTAB) and solvent isooctane resulted in purification of 2.43 fold with an activity recovery 81.3%. The reverse micellar size estimated using empirical and geometrical models indicated that the reverse micelles are large enough (R m ¼ 7.2-9.6 nm) to host bromelain molecules that are relatively smaller in size ($1.67 nm). The studies on the kinetics of mass transfer indicated a relatively slower rate (by $34%) of mass transfer in case of back extraction compared to forward extraction. Process scale-up did not significantly affect the extraction efficiency whereas purity of phase components played a major role. The mass transfer across the phases was high in the initial period of mixing for both forward and back extractions.

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Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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“…But beyond this concentration, there is a fall in protein transfer to the organic phase. This infers that very less concentration of AOT fails to form sufficient number of reverse micelles in organic phase and higher concentration leads to disruption of reverse micelles which lowers the transfer of protein to organic phase [9] .…”

Section: Effect Of Aot Concentrationmentioning

confidence: 96%

Reverse micellar partitioning of Bovine Serum Albumin with novel system

Pawar¹,

Belur²,

Iyyaswami³

2017

REFFIT

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To overcome the difficulties associated with the conventional extraction process like poor selective extrac- tion of biomolecule and scale up of the process, the reverse micellar system consist of AOT/n-heptanol was considered to extract Bovine Serum Albumin (BSA) as a model biomolecule. The maximum forward extraction of BSA from aqueous phase to micelle phase was observed at AOT concentration 160 mM, aque- ous phase pH value of 4, NaCl concentration 0.8 M and 95% back extraction of BSA from micelle phase to stripping phase was obtained at 1 M NaCl concentration with the pH of 7.5. HPLC analysis confirmed the stability of BSA during extraction. The size and water content of the reverse micelle was also reported. The obtained results emphasize the application of the AOT/n-heptanol reverse micellar system for the extraction of BSA and may be utilized for the selective extraction of similar hydrophilic proteins from the complex sources.

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“…The absorbance of onitrophenol formed by the enzyme reaction was measured in alkaline medium at 410 nm using a spectrophotometer (Shimadzu UV-160A, Japan). One unit of β-galactosidase activity was defined as the amount of enzyme that released 1 nmol of o-nitrophenol per minute under standard conditions of 37 o C and pH 4.0 for 15 min [28].…”

Section: Enzyme Assaymentioning

confidence: 99%

Membrane processing for purification and concentration of β-glycosidases from barley (Hordeum vulgare)

Hemavathi

Raghavarao

2011

Biotechnol Bioproc E

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Tangential flow ultrafiltration with a polyethersulfone (100 kDa) membrane was used for the purification and concentration of β-glycosidases (β-galactosidase and β-glucosidase) from aqueous extract of barley. The performances of mode 1 (concentration followed by diafiltration) and mode 2 (diafiltration followed by concentration) were compared. In mode 1 activity recoveries of 91.44 and 88% as well as purifications of 1.84 and 1.77-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 9 h. In mode 2 activity recoveries of 95.68 and 91.76% with purifications of 4.56 and 4.38-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 6 h. The removal of total carbohydrates and protein was 56.74 and 50.73%, respectively, in mode 1, whereas it was 81.46 and 79.04%, respectively, in mode 2. The diafiltration volume and volume concentration of 3 were maintained in both mode 1 and 2. Flux decline was severe in mode 1 and led to a long processing time of about 9 h. These results indicate that mode 2 was better than mode 1 for purification of β-glycosidases.

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Reverse Micellar Extraction of β-Galactosidase from Barley (Hordeum vulgare)

Cited by 13 publications

References 25 publications

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Reverse micellar partitioning of Bovine Serum Albumin with novel system

Membrane processing for purification and concentration of β-glycosidases from barley (Hordeum vulgare)

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